Is there any preparation needed before injecting the samples to the flow cell?

It is essential to dissolve or dilute the analyte molecule solution in the running buffer to avoid refractive index changes. For a first interaction study, it is recommended to inject different analyte concentrations from the lowest to the highest.

Complex mixtures like serum, blood or others can also be injected through the SPRi system without pre-treatment. The injection of a control sample like running buffer or a known analyte without any interaction with the ligand is advised.