What are the different steps of a SPRi experiment?

A SPRi experiment consists of three steps:

  • The ligands are immobilized on the SPRi-Biochip surface in a matrix format
  • The sample solution is passed over the SPRi-Biochip surface and variations of reflectivity are recorded in real time
  • The kinetic data is analyzed to extract quantitative information and/or kinetic rates of the interactions