Protein Quantification at Low Concentration using Fluorescence and Absorbance

Fluorescence Emission spectra of transferrin at increasing concentrations

Fluorescence Emission spectra of transferrin at increasing concentrations (from 0.75 μg/ml to 50 μg/ml)

In this application note, we highlight the high sensitivity of the Duetta for measuring transferrin at very low concentrations. The Duetta spectrofluorometer combines both fluorescence and absorbance measurements simultaneously, thus providing key advantages for protein analysis.

In parallel, we compare the fluorescence results obtained using the Duetta with absorbance results obtained with a dedicated low-volume, benchtop spectrophotometer.

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Fluorescence and Absorbance Spectrometer

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