SFA-20 Stopped Flow Device

The SFA-20/SPEX series of stopped-flow rapid-kinetics accessories offers versatility for spectroscopic monitoring of fast reactions in solution.

Fabricant: HORIBA Scientific

One of the most frequently used rapid kinetics techniques.
Used to study fast kinetics reactions (in the millisecond timescale) between two mixed reactants.
Many applications in the chemistry and biochemistry fields.
- Quenching studies
- Energy transfers
- Enzymes kinetics

Under pneumatic drive activation, the two small volumes of solutions are driven from high performance syringes through a high efficiency mixer.
The resultant mixture passes through a measurement flow cell and into a stopping syringe.
Just prior to stopping, a steady state flow is achieved.
As the solution fills the stopping syringe, the plunger hits a block, causing the flow to be stopped instantaneously.
Using appropriate techniques, the kinetics of the reaction can be measured in the cell.

Features

Dead time < 8 ms
Observation cell optimized for best performance with SPEX^® instruments
Integrates into any current SPEX^® fluorescence system
Multi-mixing ability
Flexible thermostat format
Sample’s path constructed of chemically inert Kel-F™, silica, Teflon^®, and glass
Crush-resistant flexible umbilical

Teflon^® is a registered trademark of E.I. DuPont de Nemours, Inc.

Dead Time:	<8 ms
Dead Volume:	700 µL (SFA-20 and SFA-20mx); 350 µL (SFA-20M and SFA-20mxM)
Window Sizes:	3 mm wide
Flow cell\'s beam height:	15 mm from base of cell to center of window
Min. vol. per determination:	100 µL per reactant
Observation path:	3 mm for excitation and 3 mm for emission
Observation cell:	Silica
Fill-syringe volume:	5mL
Windows:	3 mm wide x 10 mm high for excitation and emission, specifically designed for FluoroMax® and FluoroLog® instruments
Adjustable stop volume:	Available
Mixing ratio:	Up to 20:1
Temperature range:	5 - 80°C
Piston Drive:	Manual drive is standard; Pneumatic control optional. We recommend, however, the SFA-20mx/SPEX and SFA-20mxM/SPEX Umbilical length = 600 mm (SFA-20/SPEX and SFA-20mx/SPEX); 300 mm (SFA-20M/SPEX and SFA-20mxM/SPEX); other lengths on request
Drive syringes:	2.5 mL for SFA-20/SPEX; 1 mL for other models High-efficiency T-format mixer: Available for SFA-20/SPEX and SFA-20M/SPEX; 10 µL delay available for SFA-20mx/SPEX and SFA-20mxM/SPEX; longer delays upon request