Zeta Potential of Proteins
This application note presents data from the SZ-100 using cells with unique, patented carbon coated electrodes that facilitate zeta potential measurements of proteins.
nanoPartica SZ-100V2 Series
Nanoparticle Analyzer
A highly advanced analyzer solves the mysteries of the nano-world. A single device analyzes the three parameters that characterize nanoparticles: particle size, zeta potential, and molecular weight.
Nanotechnology research and development is a continuously evolving effort to control substances at the atomic and molecular level in order to achieve new and better materials and products. The miniaturization of components – that is, control at the nano-level – is necessary to achieve faster, higher-performance devices and functions and to reduce energy consumption. Nanotechnology has come to play a key role in wide-ranging fields that affect our daily lives, including food, cosmetics, and the life sciences.
Clear and simple multi-parameter analysis of nanoparticles!
Three analyzers in a single compact body deliver high-sensitivity, high-accuracy analysis of each measurement parameter.
Particle Size Measurement Range 0.3 nm to 10 µm
The SZ-100V2 Series measures particle size and particle distribution width by dynamic light scattering (DLS).
Analysis across a wide range of sample concentrations: Measurement of samples ranging from low ppm-order concentrations to high-concentration samples in double-digit percentages is possible. Accepts commercially available sampling cells. Analysis of small-volume samples is also possible.
Zeta Potential Measurement – 500 to + 500 mV
Analysis of sample volumes as small as 100 μL using HORIBA-developed microelectrophoresis cells. Use the value of zeta potential to predict and control dispersion stability. High zeta potential magnitudes indicate a stable dispersion, useful for formulation work.
Molecular weight 1 × 103 to 2 × 107 Da
Absolute molecular weight (Mw) and the second virial coefficient (A2) are obtained by performing static light scattering measurement as a function of sample concentration and preparing Debye plots.
The SZ-100V2 Series applies sophisticated intelligence and learning capability to rapidly determine nanoparticle properties!
- Since the SZ-100V2 Series analyzer covers a wide sample concentration measurement range, sample dilution and other preprocessing is nearly eliminated.
- The use of a dual optical system enables measurement of high-concentration samples such as slurry and ink pigments as well as low-concentration proteins and polymers.
- A single device analyzes the three parameters that characterize nanoparticles: particle size, zeta potential, and molecular weight.
- HORIBA-developed cells for zeta potential measurement prevent sample contamination. Simple analysis by means of ultra micro-volume dedicated cells (volume as low as 100 μL). Suitable for analysis of dilute samples.
- HORIBA-developed electrode for zeta potential cell made from carbon material, the material is not corroded by high salt samples such as saline.
SZ-100-S2 Measurement Specifications
Model | SZ-100-S2 (particle size and molecular weight measurement only) |
|---|---|
Measurement principles | Particle size measurement: Dynamic Light Scattering |
Measurement range | Particle size: 0.3 nm to 10 μm |
Maximum sample concentration | 40 wt%*2 |
Particle size measurement accuracy | Measurement accuracy of ±2% for NIST traceable polystyrene latex |
Measurement angles | 90° and 173° (automatic or manual selection) |
Cells | Cuvettes |
Measurement time | Approx. 2 min. under ordinary conditions |
Required sample volume | Minimum volume of 12 μL*3 to 1000 μL (differs depending on cell material) |
Usable liquids | Water, ethanol, organic solvents |
*1: Mark-Howink-Sakurada Equation, depending on sample.
*2: Depending on sample.
*3: F Micro-cell.
SZ-100-Z2 Measurement Specifications
(Particle size and molecular weight measurement specifications are the same as for the SZ-100-S2.)
Model | SZ-100-Z2 (with zeta potential measurement unit) |
|---|---|
Measurement principle | Zeta potential measurement: Laser Doppler electrophoresis |
Measurement range | -500 to +500 mV |
Size range suitable for measurement | Minimum 2.0 nm, Maximum 100 μm*4 |
Measurement conductivity range | 0 to 20 S/m*5 |
Maximum sample concentration | 40 wt%*6 |
Cells | Dedicated cell with electrodes |
Measurement time | Approx. 2 min. under ordinary conditions |
Required sample volume | 100 μL |
Carrier fluids | Water |
*4: Depending on sample.
*5: Recommended sample conductivity range: 0 to 2 S/m.
*6: Depending on sample.
Analyzer Specifications (SZ-100-S2 and SZ-100-Z2)
Measuring unit optical system | Light source: Diode pumped frequency doubled laser (532 nm, S2 / Z2 10 mW, HS2 / HZ2 100 mW) |
|---|---|
Laser classification | Class I |
Operating temperature and humidity | 15 - 35 °C, RH 85% or less (no condensing) |
Holder temperature control temperature settings | 0 - 90 °C (up to 70 °C for cells with electrodes and plastic cells) |
Purging | Dry gas purge port tube connection is possible. |
Power supply | AC 100 - 240 V, 50/60 Hz, 150 VA |
Dimensions | 528 (W) x 385 (D) x 273 (H) mm (excluding protrusions) |
Weight | 25 kg |
Personal computer | Windows computer with one available USB port |
Interface | USB 2.0 (between measuring unit and PC) |
OS | Windows® 10 32/64 bit |
Dimensions (mm)
Accessories
Particle size cells
| Cell name | Min volume | Solvent | |
|---|---|---|---|
| A | Disposable cell | 1.2 mL | Aqueous |
| B | Semi-micro cell | 500 µL | Aqueous, non-aqueous |
| C | Glass cell | 1.2 mL | Aqueous, non-aqueous |
| D | Semi-micro disposable cell | 600 µL | Aqueous |
| E | Cell with lid | 1.2 mL | Aqueous, non-aqueous |
| F | Micro cell (90° only) | 12 µL | Aqueous, non-aqueous |
| G | Sub-micro cell | 200 µL | Aqueous, non-aqueous |
| H | Flow cell | 100 µL | Aqueous, non-aqueous |
Zeta potential cells
Disposable zeta potential cells for aqueous measurements. Box of 20 Volume = 100 µL
Non-aquoeus zeta potential cell. Volume = 100 µL
Zeta Potential of Polyelectrolytes
The zeta potential of polyethylenimine, PEI, is measured as a function of polymer concentration with the SZ-100 Nanoparticle Analyzer. Charged polymers, that is, polyelectrolytes can be analyzed with the SZ-100.
Zeta Potential of Clay for Wastewater Treatment
In this note, the effect of coagulant choice and concentration on the treatment of suspended clays is analyzed.
DLS vs. Diffraction of Flavor Emulsions
The particle size distribution of many flavor emulsions has a substantial effect on their performance. Particle size distribution can affect mouth feel, appearance, and emulsion stability.
Wastewater Treatment Optimization with Zeta Potential
Suspended solids are a common impurity in wastewater from industrial and mining operations. In order for water clarity, the suspended solids are often allowed to settle. The duration of these processes is a strong function of the particle size. Thus, if particles can be made to aggregate, the settling process is shorter and the treatment operation is faster and less expensive.
Sizing Silica Particles by DLS
In this application note, the particle sizes of two different silica dispersions are analyzed to demonstrate the utility of the SZ-100 for both suppliers and users of such materials.
Nanoparticle Analysis
Nanotechnology is extremely diverse, ranging from novel extensions of conventional device physics, to completely new approaches based upon molecular self-assembly and to developing new materials with dimensions on the nanoscale. Materials reduced to the nanoscale can show different properties compared to what they exhibit on a macroscale, enabling unique applications.
