How to Quantify a Protein in Different Crude Samples in One Run using the XelPleX

By: Fatima-Ezzahra HIBTI*, Chiraz FRYDMAN

2 February 2019

Figure 2 Assay set-up

Author(*) Infomation: HORIBA FRANCE SAS

Abstract: Crude sample means the sample existing in a natural state and unaltered by any process. Characterizing the molecular affinities in the native medium makes it possible to understand the biological mechanisms at the molecular scales. Our systems (XelPleX) allow the analysis of the molecular binding in a complex media and generate valuable data such as determination of real-time interactions and kinetics. Indeed, the injection of complex samples without dilution, such as serum or plasma, is no longer an issue for diagnostic research or drug screening. The aim of this article is to demonstrate the capability of the Surface Plasmon Resonance imaging technology (SPRi) to detect and quantify a biomarker as an Acquired Immune Deficiency Syndrome (AIDS) diagnosis tool.

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