Fluorescence Spectroscopy
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SFA-20 Stopped Flow Device
Overview
The SFA-20/SPEX series of stopped-flow rapid-kinetics accessories offers versatility for spectroscopic monitoring of fast reactions in solution.
What is it?
- One of the most frequently used rapid kinetics techniques.
- Used to study fast kinetics reactions (in the millisecond timescale) between two mixed reactants.
- Many applications in the chemistry and biochemistry fields.
- Quenching studies
- Energy transfers
- Enzymes kinetics
How does it work?
- Under pneumatic drive activation, the two small volumes of solutions are driven from high performance syringes through a high efficiency mixer.
- The resultant mixture passes through a measurement flow cell and into a stopping syringe.
- Just prior to stopping, a steady state flow is achieved.
- As the solution fills the stopping syringe, the plunger hits a block, causing the flow to be stopped instantaneously.
- Using appropriate techniques, the kinetics of the reaction can be measured in the cell.
Features
- Dead time < 8 ms
- Observation cell optimized for best performance with SPEX® instruments
- Integrates into any current SPEX® fluorescence system
- Multi-mixing ability
- Flexible thermostat format
- Sample’s path constructed of chemically inert Kel-F™, silica, Teflon®, and glass
- Crush-resistant flexible umbilical
Teflon® is a registered trademark of E.I. DuPont de Nemours, Inc.
Manufactured by HORIBA
Specifications
Dead Time: | <8 ms |
Dead Volume: | 700 µL (SFA-20 and SFA-20mx); 350 µL (SFA-20M and SFA-20mxM) |
Window Sizes: | 3 mm wide |
Flow cell's beam height: | 15 mm from base of cell to center of window |
Min. vol. per determination: | 100 µL per reactant |
Observation path: | 3 mm for excitation and 3 mm for emission |
Observation cell: | Silica |
Fill-syringe volume: | 5mL |
Windows: | 3 mm wide x 10 mm high for excitation and emission, specifically designed for FluoroMax® and FluoroLog® instruments |
Adjustable stop volume: | Available |
Mixing ratio: | Up to 20:1 |
Temperature range: | 5 - 80°C |
Piston Drive: | Manual drive is standard; Pneumatic control optional. We recommend, however, the SFA-20mx/SPEX and SFA-20mxM/SPEX Umbilical length = 600 mm (SFA-20/SPEX and SFA-20mx/SPEX); 300 mm (SFA-20M/SPEX and SFA-20mxM/SPEX); other lengths on request |
Drive syringes: | 2.5 mL for SFA-20/SPEX; 1 mL for other models High-efficiency T-format mixer: Available for SFA-20/SPEX and SFA-20M/SPEX; 10 µL delay available for SFA-20mx/SPEX and SFA-20mxM/SPEX; longer delays upon request |
