Fluorescence Spectroscopy
News
- HORIBA Scientific Presents Young Fluorescence Investigator Award at Biophysical Show. Read more
- HORIBA Scientific Announces New Ultima TCSPC lifetime system, the next generation of turnkey TCSPC Fluorescence Lifetime systems...Read more
- HORIBA Scientific announces the acquisition of the Photon Technology International (PTI) Business... Read more
- HORIBA Scientific Announces New Generation of Turnkey TCSPC Fluorescence Lifetime Systems...Read more
- Aqualog CDOM Fluorometer Wins Award as one of Best New Instruments in 2011...Read more
- HORIBA Scientific and NKT Photonics Introduce the World’s First Commercial Integrated Supercontinuum Powered Spectrofluorometers... Read more
- Aqualog®, the only simultaneous absorbance and fluorescence system, measures CDOM (colored dissolved organic matter) in water 100 times faster than previous fluorescence methods...Read more
- World's First 100MHz Picosecond Diode Source With USB Interface and Plug-and-Play Interchangeable Heads ...Read more
Steady State Solutions
Overview
HORIBA Scientific’s confocal, universal fiber coupling solution allows any of our steady state and hybrid fluorometers to be easily connected to virtually any upright or inverted microscope. Spectrofluorometric capabilities include the unparalleled precision and sensitivity of Single-Photon Counting and the speed and sensitivity of CCD detection. Similarly, fluorescence spectral mapping capabilities utilize an automated fast scanning X-Y stage to generate rapid spectral maps with micron level spatial resolution. Of course our microscope solutions preserve the microscope's native optical performance and capabilities. For example, they add no optics to the entire emission optical path. Use the high performance cooled fluorescence camera to image your field of interest. Once you identify a region to spectrally analyze, simply switch your filter cube to a beam splitter or an appropriate dichroic, divert the light to the fluorometer, and you are collecting high resolution spectra. Custom solutions are also available to measure in the UV and NIR regions, beyond the normal range of your microscope.
Couple a microscope and:
- Measure complete spectra of samples as small as 1 μm.
- Get spectra from as little as a few molecules of sample.
- Perform localized FRET measurements.
Add an automated stage and camera, and:
- Create complete spatial/spectral maps.
- Perform repetitive QC characterization of structured samples like photovoltaics.
Manufactured by HORIBA
Specifications
Specification | Complete Microscope Solution | Interface to Existing Microscope |
|---|---|---|
Objective | Plan achromat x10 and x60, other magnifications available. 6 position turret | User supplied |
Confocal pinholes | Kinematic, 6 diameters, 0.1, 0.2, 0.4, 0.6, 0.8, 1 mm | Kinematic, 6 diameters, 0.1, 0.2, 0.4, 0.6, 0.8, 1 mm |
Dimensions, Weight | 68 cm x 46 cm x 60 cm, 40 kg (BX53) | |
Excitation source: Scopelite 200 (Optional) | ||
Wavelength range | From 350 to 700 nm | From 350 to 700 nm |
Illumination Adjustment | 5 preset % apertures: 0, 25, 50, 75, 100 | 5 preset % Apertures: 0, 25, 50, 75, 100 |
Output Power | 7 Watts | 7 Watts |
Lamp Life | 2000 hrs | 2000 hrs |
Warm Up | 2 min | 2 min |
Long Term Drift | <0.1% RMS | <0.1% RMS |
Cooling | Air cooled by fan | Air cooled by fan |
Dimensions | 20 x 14.5 x 25.4 cm (WxDxH) | 20 x 14.5 x 25.4 cm (WxDxH) |
Weight | 3.4 kg | 3.4 kg |
Filters | ||
ND filter sliders | 6 positions: 0, 0.3, 0.6, 1, 2 and 3 OD | User supplied |
Fibers | ||
Multimode slit to round | UV-VIS : 1.5 m length, 250-850 nm wavelength range | UV-VIS : 1.5 m length, 250-850 nm wavelength range |
Fiber Coupling | ||
Excitation | Collimating lens adapters for Olympus | Collimating Lens Adapters for Olympus, Nikon, Leica and Zeiss |
Emission | Universal C-mount adapter with pinhole holder | Universal C-mount adapter with pinhole holder |
Camera | Required for mapping option | Required for mapping option |
Fluorescence camera | 12 bits, 1.4 Mpix, cooled, low noise | 12 bits, 1.4 Mpix, cooled, low noise |
Motorized stage | Required for mapping option | Required for mapping option |
Repeatibility | <1.0 µm | Suitable stage |
Software/Computer | ||
FluorEssence | Windows® 7, 32- or 64-bit | Windows® 7, 32- or 64-bit |
