What are the different phases of a kinetic curve?

When the sample solution is injected, the .analyte must reach the flow cell to interact with the ligand. This period of time is called the latency time (A).

When the sample is injected, it is the association phase (B). As the analytes go through the flow cell, they interact with the ligands on the surface.

When all ligands are involved in an interaction with the analytes, it is the saturation phase (C).

When no more analytes go through the flow cell, the analyte solution is replaced by the running buffer, and analytes dissociate from the immobilized ligands, it is the dissociation phase (D).

Some interactions have a high affinity and some analytes may still bind to the ligands after the dissociation step, in this case, a regeneration solution is injected to remove all the remaining analytes. This is the regeneration step (E).  A new sample solution can then be injected.

Example of a kinetic interaction curve: (A) latency time, (B) association, (C) saturation, (D) dissociation, (E) regeneration.
Example of a kinetic interaction curve: (A) latency time, (B) association, (C) saturation, (D) dissociation, (E) regeneration.