Surface Plasmon Resonance imaging (SPRi)
Reagents for label-free interaction analysis
Our SPRi reagents have been especially designed for our SPRi systems. They allow you to work with confidence and save time for faster results.
SPRi Student kits
The SPRi starting kits are used to get acquainted with multiplex SPR imaging. Choose from two biological models and learn how SPRi can save you time and money.
Product name | Description |
|---|---|
Protein biochip kit | Ready-to-use SPRi-Biochips and necessary reagents to perform a antibody-protein interaction |
DNA biochip kit | Ready-to-use SPRi-Biochips and necessary reagents to perform a DNA-DNA interaction |
SPRi Running buffers
Depending on the ligand/analyte interaction study, the composition of the running buffer is expected to improve specific binding. Salted buffers like PBS or HEPES are recommended. Buffer composition, concentration or pH must be adjusted according to the biomolecules studied.
| Product name | Description |
|---|---|
| PBS | 0.01 M PBS pH 7.4 137 mM NaCl 2.7 mM KCl |
| PBS-T | 0.01 M PBS pH 7.4 137 mM NaCl 2.7 mM KCl 0.005% (v/v) Tween 20 |
| PBS 10X | 0.1 M PBS pH 7.4 1.37 M NaCl 0.027 M KCl |
| PBS-T 10X | 0.1 M PBS pH 7.4 1.37 M NaCl 0.027 M KCl 0.05% (v/v) Tween 20 |
| TBS 5X | 0.05 M TBS pH 7.4 150 mM NaCl |
| TBS-T 5X | 0.05 M TBS pH 7.4 150 mM NaCl 0.005% (v/v) Tween 20 |
| HBS | 0.01 M HEPES pH 7.4 150 mM NaCl |
| HBS-T | 0.01 M HEPES pH 7.4 150 mM NaCl 0.005% (v/v) Tween 20 |
| HBS-E | 0.01 M HEPES pH 7.4 150 mM NaCl 3 mM EDTA |
| HBS-ET | 0.01 M HEPES pH 7.4 150 mM NaCl 3 mM EDTA 0.005% (v/v) Tween 20 |
| SPRi buffer kit 1 | Contains 4 buffers without Tween |
| SPRi buffer kit 2 | Contains 4 buffers with Tween |
SPRi Calibration solution
The calibration solution helps correct for sensitivity dispersions on the sensor chip surface.
| Product name | Description |
|---|---|
| N-SUC | 3 mg sucrose to reconstitute in 1 mL of running buffer |
SPRi activation kits
The SPRi activation kits should be used for primary amine coupling chesmitries with COOH groups ready to be activated (SAM CH-HD and CH-LD and 3D chemistries)
| Product name | Description |
|---|---|
| Amine coupling kit 1 | Necessary reagents for NHS activation of COOH groups for amine coupling. To be reconstituted in 1 mL of filtered and deionised water. Chemistry activation using NHS is more stable but less reactive. |
| Amine coupling kit 2 | Necessary reagents for Sulfo-NHS activation of COOH groups for amine coupling. To be reconstituted in 1 mL of filtered and deionised water. Chemistry activation using Sulfo-NHS is more reactive but less stable |
SPRi Blocking solutions
The SPRi blocking solutions are used for deactivating the remaining reactive groups of the surface chemistry for covalent coupling. They can also help reduce non-specific binding.
| Product name | Description |
|---|---|
| B-Glycine | 10 mM Glycine in PBS |
| B-Ethanolamine | 1M Ethanolamine-HCl pH 9 |
| B-BSA | 1% BSA in PBS-T |
SPRi Regeneration solutions
The SPRi regeneration solutions are used to remove the bound analyte and any other non-covalently bound material while keeping the ligand activated. Regeneration conditions (concentration, contact time, pH…) must be optimized for each new ligand-analyte interaction study.
| Product name | Description |
|---|---|
| Glycine-HCl | 100 mM Glycine - HCl pH 2 |
| SDS | 10% SDS |
| NaOH | 50 mM NaOH |
| HCl | 100 mM HCl |
| NaCl | 1 M NaCl |
| SPRi Regeneration scouting kit | Contains 5 regeneration solutions, for exploring regeneration conditions. |
SPRi Cleaning solutions
The SPRi cleaning solutions are used for the daily cleaning and monthly maintenance of the SPRi systems.
| Product name | Description |
|---|---|
| C-NaOH | 100 mM NaOH (for the monthly maintenance) |
| C-HCl | 100 mM HCl (for the monthly maintenance) |
| SDS | 10% SDS (for the daily cleaning) |
| SPRi Cleaning kit | Contains 4 cleaning solutions |
