Fluorescence Spectroscopy


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Aqualog® - Our compact, Benchtop Fluorometer for CDOM



New A-TEEM Technology

New Datastream Dashboard Software

Aqualog is the only instrument to simultaneously measure both absorbance spectra and fluorescence Excitation-Emission Matrices. EEMs are acquired up to a 100 times faster than with other instruments. Dedicated software automates traceable Quinine Sulfate Unit calibration and correction of inner-filter effects and Rayleigh and Raman scattering lines, enabling rapid export to modeling algorithms like PARAFAC.

multivariate analysis - AqualogAqualog now offers new deep UV absorbance and fluorescence excitation from 200 nm, in addition to a fully automated sample queue tool to facilitate continuous data acquisition, analysis and export for immediate multivariate analysis with the Solotm package from our partner, Eigenvector Research, Inc.

The NEW Aqualog® Datastream Dashboard facilitates completely automated analysis and reporting of a wide range of organic matter parameters that are critical for managing and optimizing the drinking water treatment process. The Datastream parameters have been selected to specifically target disinfection by-product issues, algal issues and other contamination components 




  • The only true simultaneous absorbance-fluorescence system available

  • TE-cooled CCD fluorescence emission detector for rapid data acquisition up to 100 times faster than any other benchtop fluorometer

  • Corrected UV-VIS absorbance detection path for stability and accuracy

  • Double grating excitation monochromator for superior stray light rejection

  • Matching bandpass for absorbance and fluorescence spectra

  • Automatic sample changer option (2 or 4 position)

  • Compatible with flow cells and titrator


  • Optimized experiment set-up menus minimize user confi guration time

  • Complete NIST-traceable corrected fluorescence spectra automatcally generated

  • Spectral and kinetic analysis tools for both absorbance and fluorescence data

  • Methods and batch protocols for automating multiple sample measurement

'How Fast is Fast?'

Click here for the Aqualog 3.6.11 Patch.
Please Note: Please select to save the file. Release Notes.



New A-TEEM technology

A-TEEM technology uses absorbance, transmittance and EEM data to fingerprint molecules with high specificity and ultrahigh-sensitivity at a 6 million nm/min emission scan rate!

All colored molecules exhibit unique molecular absorbance and transmittance spectra; many colored molecules also exhibit unique fluorescence excitation and emission spectra that can be measured as an excitation-emission matrix (EEM). Simultaneously combining Absorbance–Transmission and EEM is a new technique (A-TEEM) that provides a distinct molecular fingerprint with numerous potential applications.

Aqualog combines an ultrafast CCD that’s up to 4,000 times faster than traditional PMT-based fluorometers, with our new A-TEEM technology so you can easily and effectively identify, quantify and understand individual organic compounds in complex mixtures in minutes. A-TEEM has already proven in many cases to be more effective in protein, vaccine, wine and water research, quality and process applications than HPLC and vibrational spectroscopy.


NEW Aqualog® Datastream Dashboard


  • Seamless integration with Aqualog
  • Convenient HTML-based interface
  • Push-button method operation
  • Simple administrator level controls for calibration and method development


  •  Easy access through internet or intranet
  •  Dashboard shows the latest readings, time series and tables for trends and analysis
  •  WTP can upload their own independent data

Eigenvector Research, Incorporated Aqualog Datastream Dashboard is powered by Solo Predictor software from
Eigenvector Research, Incorporated

Manufactured by HORIBA


Aqualog Specification

Datastream Dashboard Specification

Aqualog Specification

Fluorescence Hardware

Choice of Light source

Standard: 150W ozone-free vertically mounted xenon arc lamp

Extended-UV: 150W vertically mounted xenon arc lamp

Excitation range

230 nm to upper limit of emission detector

200 nm to upper limit of emission detector

Excitation bandpass

5 nm

Excitation monochromator

Subtractive double monochromator

Excitation gratings

1200 gr/mm, 250 nm blaze

Excitation wavelength accuracy

±1 nm

Choice of Detector



Emission range

250-620 nm

250-800 nm

Emission grating

405 gr/mm; 250 nm blaze

285 gr/mm; 350 nm blaze

Hardware pixel binning

0.41, 0.82, 1.64, 3.28 nm/pixel

0.58, 1.16, 2.32, 4.64 nm/pixel

Emission bandpass

5 nm

Emission spectrograph

Fixed, aberration-corrected 140 mm focal length

Emission detector

TE-cooled back-illuminated CCD

Emission integration time

5 ms minimum


CCD gain options

2.25 e-/cts in high gain, 4.5 e-/cts in medium gain, 9 e-/cts in low gain


Water-Raman SNR > 20,000:1 (RMS method) (350 nm excitation, 30s integration)


32.72 kg (72 lbs)


LWH (618 x 435 x 336 mm); (24" x 17" x 13")

Absorbance Hardware

Scanning range

200 - 800 nm (UV lamp)
230-800 nm (Standard lamp)


5 nm

Slew speed

Max 500 nm/s

Optical system

Corrected single-beam


Si photodiode

Wavelength accuracy

±1 nm

Wavelength repeatability

+/- 0.5 nm

Photometric accuracy

±0.01 AU from 0 to 2A

Photometric stability

<0.002 AU per h

Photometric repeatability

 +/- 0.002 AU (0 to 1 AU)

Stray light


<1% measured with KI standard

Datastream Dashboard Specification


Aqualog Datastream Parameters



Dissolved Organic Carbon Concentration

30 µg/l-20 mg/l

Requires Filtration (0.45 µm)

A254 nm

1 cm path length


L  DOC mg-1
 A254 m-1

Simulated Distribution, System Trihalomethane,
Formation Potential

SDS THMFP (10 µg/l – 500 µg/l)

SDS THMFP (10 µg/l – 500 µg/l)

Parallel Factor Analysis Component Scores

Up to 7

Can include Algal, Oil/PAH, Tracer Dyes and other Components

Residuals (Q)

Detect Contaminants and Measurement Issues

% Variance Accounted For

Detect Contaminants and Measurement Issues

Absorbance Spectrum

200-800 nm; 1 cm path length

Any wavelength coordinate or ratio can be analyzed with extinction coefficients

Excitation Emission Matrix (EEM) Regions

I-V plus Algal (Bluegreen/Brown/Green)

Custom EEM regions also available; Based on Interpolated EEM

Total Fluorescence

Sum of EEM Regions I-V

Humic Index

Fluorescence Index

Independent Treatment Plant Data





Chlorine Residual




A254 nm

Adjustable Path Length Specifications


Additional Parameters Available

System Requirements

Datastream Specifications

Windows OS

64 bit Windows 7 or higher

Web Browser

Google Chrome ≥V53.x or Firefox  ≥49.x

Memory (RAM)

≥ 4 GB

Hard Disk

≥ 4 GB



USB Ports

≥ 2

Additional ports may be required for accessories

Video Resolution

≥ 1024 x 768

Eigenvector Solo Industrial

V8.2 or higher

HORIBA Onsite Installation/Calibration


HORIBA 6 month service


License Period

1 year renewable

Aqualog Hardware

UV-800 (200-800 nm)

Eigenvector Research, Incorporated Aqualog Datastream Dashboard is powered by Solo Predictor software from
Eigenvector Research, Incorporated

Fluorescence webinar
OpenFluor Webinar