Fluorescence Spectroscopy

News

  • HORIBA Scientific Presents Young Fluorescence Investigator Award at Biophysical Show. Read more
  • HORIBA Scientific Announces New Ultima TCSPC lifetime system, the next generation of turnkey TCSPC Fluorescence Lifetime systems...Read more
  • HORIBA Scientific announces the acquisition of the Photon Technology International (PTI) Business... Read more
  • HORIBA Scientific Announces New Generation of Turnkey TCSPC Fluorescence Lifetime Systems...Read more
  • Aqualog CDOM Fluorometer Wins Award as one of Best New Instruments in 2011...Read more
  • HORIBA Scientific and NKT Photonics Introduce the World’s First Commercial Integrated Supercontinuum Powered Spectrofluorometers... Read more
  • Aqualog®, the only simultaneous absorbance and fluorescence system, measures CDOM (colored dissolved organic matter) in water 100 times faster than previous fluorescence methods...Read more
  • World's First 100MHz Picosecond Diode Source With USB Interface and Plug-and-Play Interchangeable Heads ...Read more

FluoroMax Series

Overview

  • Features

  • Hardware

  • FluorEssence Steady State and Frequency Domain Software

  • DAS6 Decay Analysis Software

  • DataStation TCSPC Acquisition Software

  

Features

  • Water Raman signal-to-noise ratio: 16000:1 (6000:1 FSD)
  • Photon Counting for Ultimate Sensitivity
  • Fast Scanning capability - up to 80 nm/second
  • Powerful FluorEssence™ software for Windows®
  • Method Files to recall complete experiment parameters
  • Time-correlated single-photon counting (TCSPC) accessory for lifetime determinations
  • Phosphorimeter accessory for long-lived luminescence determinations

 

Hardware

The Source

The xenon source that supplies prime UV performance is focused onto the entrance slit of the excitation monochromator with an elliptical mirror. Besides ensuring efficient collection, the reflective surface keeps all wavelengths focused on the slit.

As an option an additional, software-selectable, xenon flash lamp can be fitted to enable phosphorescence studies.

The Slits

The slits themselves are bilaterally, continuously adjustable from the computer in units of bandpass or millimeters. This preserves maximum resolution and instant reproducibility.

The Excitation Monochromator

The excitation monochromator is an aspheric design which ensures that the image of the light diffracted by the grating fits through the slit. The gratings themselves are plane, blazed gratings that avoid the two major disadvantages of the more common concave holographic gratings: poor polarization performance and inadequate imaging during scans that throws away light. The unique wavelength drive scans the grating at speeds as high as 80 nm/s. The grating grooves are blazed to provide maximum light in the UV and visible region.

The Reference Detector

Before the excitation light reaches the sample, a photodiode reference detector monitors the intensity as a function of time and wavelength. The photodiode detector has a wider wavelength response than the older, traditional rhodamine-B quantum counter, and requires no maintenance.

The Sample Chamber

A spacious sample chamber is provided to allow the use of a long list of accessories for special samples, and encourages the user to utilize a variety of sample schemes.

The Emission Monochromator

All the outstanding features of the excitation monochromator are also incorporated into the emission monochromator. Gratings are blazed to provide maximum throughput in the visible region.

The Emission Detector

Emission detector electronics employ photon-counting for the ultimate in low-light-level detection. Photon-counting concentrates on signals that originate from fluorescence emission, ignoring smaller signals originating in the detector tube (PMT). The more common method of analog detection (used by lower performance fluorometers) simply adds noise and signal together, masking weak emissions.

The emission detector housing also contains an integral high-voltage supply which is factory set to provide the signal-to-noise ratio.

Computer Control

The entire control of the FluoroMax originates in your PC with our FluorEssence™ software and is transmitted through a USB link. On start-up, the system automatically calibrates and presents itself for new experiments, or stored routines instantly called from memory.

Accessories

A wide variety of accessories are applications-oriented and most are interchangeable with the FluoroMax system.

 

FluorEssence Steady State and Frequency Domain Software

FluorEssence - Steady State and Frequency Domain Software

FluorEssence™ for Windows® sets a new standard for fluorescence software. FluorEssence™ merges spectrofluorometer data-acquisition directly to the famous processing power of Origin® software.

Simple clicks let you select a scan type, accessory or bring back a complete experimental set-up.

Revolutionize the Way You Operate Your Spectrofluorometer:

  • Simplified windows make data-acquisition intuitive even to the casual user.
  • With detector algebra, assemble input signals from different detectors into unique equations (including arithmetic or transcendental functions) that produce data in a format tailored to your application.
  • Select the special Real Time Control (RTC) window to optimize your run parameters before actually scanning.
  • Free video tutorials make FluorEssence™ even easier to learn.
  • Run a variety of accessories including polarizers, microwell-plate readers, temperature baths, autotitrators, phosphorimeters, and more.
  • Automatic correction for blanks and lamp output.
  • Change units for temperature, wavelength, and slit-width with the click of a mouse.

FluorEssence™ also includes the powerful processing and data-management tools of Origin® to streamline your laboratory’s operation.

Data-viewing, plotting, processing and management are all available, including extensive import and export facilities, view manipulation and creation, arithmetic applications, peak-picking and integration, data- management and much more!

Software Features

  • Data views in Workbook formats, keeping graphs, tables and notes together for each experiment
  • Zooming and scaling
  • Contour maps and profiles from 3D plots
  • Integration/DerivativeFit fluorescence data to Gaussian, Lorentzian, and custom curves
  • Standard arithmetic
  • 3D perspective
  • Smoothing
  • Deconvolute
  • Excitation/emission correction
  • Interpolation and extrapolation
  • Blank subtraction
  • Normalization
  • PLQY calculator wizard (for use with Quanta-Phi accessory)

 

DAS6 Decay Analysis Software

HORIBA Scientific decay analysis software recovers kinetic information (such as fluorescence lifetime and rotational correlation times) from luminescence decay data by fitting the raw decay data to one of a selection of kinetic models. Features such as shift iteration and reconvolution of instrument response function (IRF, or "lamp" profile) allow accurate recovery of multiple decay components even when the decay data is grossly distorted by the IRF.

DAS6 is designed to streamline the analysis of time-domain luminescence data, while still allowing for fine-tuning of parameters where necessary.  Analysis modules include reconvolution analysis of multi- exponential models plus analysis of more specialised fluorescence decay processes as detailed below.

Multi-Exponential

Analysis for up to 5 exponentials.

Batch Exponential

Batch mode supports datasets containing up to 10,000 decays and is ideal for applications where large amounts of lifetime data are generated e.g. lifetime imaging and reaction monitoring.  It features full reconvolution with up to five exponentials and shift iteration.

Global Exponential

Perform global analyses of up to 5 exponential components and 100 decay curves.

Distribution

Two methods are available for performing lifetime distribution analysis.  The first method employs a “Top Hat” distribution function which allows a single lifetime distribution to be fitted in conjunction with an additional discrete lifetime.  The second method is an optional module which will perform a Non-Extensive Decay distribution which allows up to five lifetime distributions to be modelled.

Exponential Series

Exponential series analysis of up to 30 terms.  This allows parameterisation of otherwise difficult data.

Foerster Energy Transfer

2D and 3D Foerster type energy transfer with an optional exponential component.

Yokoto-Tanimoto Energy Transfer

Yokoto-Tanimoto energy transfer taking into account diffusion with an optional exponential component.

Micellar quenching

Micellar quenching kinetics can help determine the surfactant aggregation number.

Exciplex

Exciplex analysis follows formation of an excited state species.

Anisotropy analysis

Impulse reconvolution of up to 2 correlation times and 5 fluorescence decay components.  Each model parameter, including shift, can be fixed at a pre-determined value or optimised as part of the parameter fitting.  The use of reconvolution allows accurate determination of decay times and rotational correlation times even when the observed decay is grossly distorted by the duration of the excitation pulse.

 

DataStation TCSPC Acquisition Software

DataStation is the latest measurement environment for time-domain fluorescence and is supplied with all HORIBA Scientific lifetime systems.

Lifetime systems are made up of various modules and components, including pulsed light sources, PMTs, monochromators, motorized polarizers, focusing optics, MCAs etc. DataStation integrates the control of all these components into one intuitive, mouse-driven Windows environment.

Lifetime systems can perform a variety of measurement functions, such as fluorescence decay measurement, phosphorescence decay measurement, time-resolved anisotropy measurements, steady-state spectra, time-resolved emission scan (TRES), temperature scans and more. These measurements are now routine using DataStation. Measurement templates are provided to quickly set up the instrument for the required measurement, and automated data acquisition is performed using computer controlled components.

Acquired data can be saved in a variety of formats including the .das format, HORIBA Jobin Yvon IBH v4.2, and ASCII, printed to your Windows printer, exported or copied to the clipboard for manipulation using a third-party application.

DataStation can be purchased separately for use on non-HORIBA Scientific systems, and we are happy to add custom functionality and interfacing (e.g. to additional monochromator types, motorized polarizer holders etc).

The DataStation software comes into its own when combined with a FluoroHub.

Functionality

DataStation evolves continually as new functionality is added in response to customer suggestion. The following is a partial list:

Graphical

  • Log or linear y-axis.
  • Auto scaling y-axis.
  • Multiple mouse zoom modes - x only, y only, x and y, pan, reset.
  • Reversed time-axis mode (automatically reverses data during reversed-TAC mode measurements).
  • Live display of cursor position, peak count, peak channel and total count.
  • Detachable and customizable toolbars with helpful hints.
  • Context-sensitive pop-up toolbars.
  • Intuitive tree navigation for files and hardware settings.
  • Tree navigation control window for FluoroHub control.
  • Real-time estimated average lifetime during acquisition.

 

TCSPC mode

  • Measurement presets - peak count or elapsed time.
  • Multiple traces on screen simultaneously.

TRES mode

  • Configure instrument for scan operation in just a few mouse clicks.
  • Scan excitation or emission monochromator.
  • Records 3D data (counts vs time vs wavelength) with live 3D progress display and interactive rotation.
  • Manual and automatic data slicing.

Anisotropy mode

  • Target difference count.
  • G-factor measurement.

MCA support

  • Canberra/Tennelec PCA3.
  • EG&G/Perkin Elmer Ortec Trump card.

Monochromator support

Polarizer support

  • HORIBA Scientific 5000U-02 polarizers.
  • PRA polarizers.

Temperature control support

  • HORIBA Jobin Yvon IBH dipstick temperature controller (as accessory to HORIBA Scientific 5000 series system only).
  • Cryostat (as accessory to HORIBA Scientific 5000 series system only).

System Requirements

The following PC specification are required to run DataStation. Measurement functionality depends on spectrometer hardware attached. Basic operation requires a supported MCA card or FluoroHub. Contact HORIBA Scientific for more information.

1 Gb RAM, 1 Gb HD space, DVD drive, 1024x768 graphics with minimum 256 color support, mouse or other pointing device. Windows™ XP or later (XP/Vista/7). Windows™ XP recommended.


Manufactured by HORIBA Scientific

Specifications

Lamp

Vertically mounted, CW, 150 W Ozone-free xenon arc lamp

Gratings

1200 groove/mm blazed at 330 nm (excitation) and 500 nm (emission), plane ruled

Automatic self-calibration of all wavelength drives and slits

Monochromators

All reflective optics, Czerny-Turner spectrometers

Detectors

Emission: R928P photon counting PMT (185-850 nm) and reference photodiode for monitoring lamp output

Water Raman S/N

6,000:1 (FSD method) 16,000:1 (RMS method) See Signal-to-noise ratio

Slits

Continuously variable from 0 to 30 nm

Accuracy

0.5 nm

Repeatability

0.1 nm

Minimum step

0.0525 nm

Integration time

0.001 to 160 sec

Software

FluorEssence

Spectral Correction Factors

Included

Brochures

FluoroMax Series

 

Software Downloads

Click here for the FluorEssence™ 3.x Patch. (only for version 3.x and above)Please Note: Please select to save the file. Release Notes.
Last Updated : 9th September 2015

Click here for the FluorEssence™ 3.x Patch. (Japanese Version)
Please Note: Please select to save the file.
Last Updated : 9th September 2015

Click here for the FluorEssence™DS 3.x Patch for systems with TCSPC. (only for version 3.x and above)
Please Note: Please select to save the file.
Release Notes.
Last Updated : 9th September 2015

Click here for the FluorEssence™ 2.5.2 Patch.
Please Note: Please select to save the file.

Click here for the FluorEssence™ 2.5.2 Patch (Japanese Version).
Please Note: Please select to save the file.

Click here for the FluorEssence™DS 2.5.2 Patch for systems with TCSPC.
Please Note: Please select to save the file.

Accessories

Microsense microliter fluorescence measurement solution

Microsense lets you make accurate, repeatable fluorescence measurements of µL volumes, less than 1/300th of a typical sample!

Simply pipette your sample. No dilution means it can easily be recovered.

Microsense is easy to...

Quanta-phi

When combined with a HORIBA Fluorometer the Quanta-φ forms the most accurate, versatile and complete luminescence yield system available!

MicroMax 384 Microwell Plate Reader

The MicroMax 384 is a microwell-plate reader able to accept plates with up to 384 wells, and connect to FluoroMax® or FluoroLog® spectrofluorometers.

Auto Titration Injector F-3005 and F-3006

The AutoTitration Injector is used for running automated and batch titrations with precisely controllable and programmable aliquot sizes and mixing speeds.

SFA-20 Stopped Flow Device

The SFA-20/SPEX series of stopped-flow rapid-kinetics accessories offers versatility for spectroscopic monitoring of fast reactions in solution.

FL-1013 Liquid Nitrogen Dewar Assembly

For phosphorescence or delayed fluorescence measurements, it is sometimes desirable to cool samples to liquid-nitrogen temperature. The FL-1013 LN dewar accessory enables these measurements with Fluorolog and FluoroMax systems.

Adapter for 50µl microcell

Adapter for 250µl microcell

HPLC Flow Cell 20µl

Cylindrical Quartz Microcell 50µl or 250µl

Cylindrical Quartz Microcell 50µl

Reduced Volume Cell 1ml, 5 x 5mm. Includes adapter and magnetic stirrer

Quartz Cuvette, 4ml, capped or stoppered

Liquid Nitrogen Dewar Assembly

Fiber Optic Platform

Stopped-Flow accessory, for rapid kinetics studies

  • Total Internal Reflectance
  • Emission Shutter (lid activated)
  • Fiber Optic Bundle, 1m, 2m or 5m, bifurcated, randomized
  • Cut-on Filter Set
  • Filter Kit Set with holder
  • Filter Holder
  • Four Position Holder, Automated, Thermostatted with Magnetic stirrer
  • Single Cell Holder, Thermostatted, Front Face with Magnetic Stirrer
  • Solid Sample Holder
  • Thermostatted Single Cell Holder with Magnetic Stirrer
  • Injection Port
  • Red Sensitive Photomultiplier Tube (R928P)
  • Polarization Accessory
  • Manual L Formatted Polarization Accessory
  • R2658 near-IR detector

Fluorescence Webinar
OpenFluor Webinar

Support Documents

Brochure