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Overview

PTI QuantaMaster™ 400 – Open Architecture Steady-State Fluorescence System


The world’s famous, versatile modular design from PTI, very high sensitivity (S/N=20,000:1 by FSD method), allows for additional light sources and detectors (from UV to NIR), supports various options for phosphorescence and fluorescence lifetimes. Features asymmetrical, aberration-corrected 300 mm monochromators with high throughput and low stray light.

Light Source

General Information

The PTI QuantaMaster 400 is unique in offering various light sources to best meet your needs. The standard light source is the 75 W xenon lamp, which acts as an almost perfect point source, making it ideal for delivering a lot of optical power to a small area, such as the slit of a monochromator. In addition it is an excellent broadband light source in the UV-VIS portion of the spectrum, reasonably continuous throughout the region from 240 to 1200 nanometers and useable up to about 2000 nm. An optional 75W extended UV version xenon lamp in the will go down to 180 nm.

In addition to the 75W xenon lamp, the standard housing can accommodate 100W Mercury, 100W Tungsten-Halogen, 150W Xenon and 200W Mercury-Xenon lamps.

The Lamp Housings

The QM-400 sealed lamp housing requires no ozone venting, and the 75-watt arc lamp is air-cooled. The integrated arc lamp power supply and igniter is virtually RF emission free.

Spectroscopic performance of an instrument does not depend on the wattage of the lamp alone; rather it is the power that gets to your sample that matters. Thanks to its proprietary thermally matched and high efficiency collection optics with ellipsoidal reflector, the QuantaMaster source rivals higher power sources in terms of power delivered to the sample, when used in conjunction with a monochromator.

ECO-Friendly

Less power in for an equivalent useful power onto your sample means more efficient use of energy. Along with the sealed housing with no ozone pollution, these make the PTI QuantaMaster sources the most ECO-friendly.

 

Monochromators

General Information

The QM-400 uses 300mm, asymmetrical Czerny-Turner type, coma-aberration corrected, low stray light monochromators. All monochromators come with a triple grating holder driven by anti-backlash direct-drive. The monochromators are auto calibrated and under computer control.

An optional internal shutter is available for the excitation monochromator. It is useful for reducing photo bleaching.

Optical Path Configuration Options

The monochromators can accommodate up to two inputs and two outputs. Folding and flipping mirrors create a variety of configurations for multiple illumination/detection applications. The figure at right shows all of the configurations that are possible. Slashes (/) represent folding mirrors, and X's represent optional flipping mirrors.  The flipping mirrors are under computer control. Monochromators are shipped with both entrance and exit folding mirrors (remove one or both to change configuration).

All monochromators are shipped with two continuously adjustable manual digital slit assemblies – computer controlled slits are available as options. Each optional flipping mirror includes an additional slit assembly.

Note that excitation and emission monochromators are not the same. Each type of monochromator has been optimized for emission specific function, providing the ultimate in throughput with the lowest stray light.

 

Triple Grating Backlash-free Grating Turret

The new 300 mm monochromators come standard with computer controlled triple grating backlash-free grating turret. The excitation monochromator includes a 1200 l/mm 300 nm blazed 68 x 68 mm grating, the emission monochromator, a 1200 l/mm 400 nm blazed grating.

These gratings can be exchanged at time of order to any of our standard ruled gratings at no extra charge, or for holographic or special order gratings at a modest additional charge.

You can also add up to two additional gratings to fully populate the triple grating holder at an additional charge. These can either be optimized for a different spectral range or give additional resolution options.

 

Adjustable Slits

The standard systems come with micrometer adjustable slits. The PTI QuantaMaster is the only commercial fluorometer to offer this choice. Optional computerized slits are available. While computer controlled slits are more convenient, many users still prefer the micrometer adjustable slits. Slit width is continuously adjustable from 0 to 6 mm. The band pass depends on the grating used.

 

Sample Compartment

The QuadraCentric™ standard sample compartment offers: a 10 x 10 mm thermostatable cuvette holder equipped with a variable speed stirrer, high efficiency quartz optics, filter holders, real time excitation correction, lid activated emission shutter and one quartz cuvette. In addition, the large sample compartment allows plenty of room for numerous options, such as polarizers, solid or powdered sample holder, a LN dewar and other accessories.

Standard Features

  • Model MP-1 QuandraCentric™ (L-format, dual L-format, or T-format) sample compartment
  • Excitation and emission filter holders (filters not included)
  • Quartz excitation/emission lenses (f/1.3)
  • Lid-activated emission port shutter
  • thermostatable 10 x 10 mm cuvette holder (external bath not included)
  • Motorized sample stirrer

 

AccuCheck™ Excitation Correction

A calibrated photodiode is used to correct for the spectral distribution of the excitation channel as well as for intensity fluctuations of the lamp. The correction is done in real time, which guarantees excellent accuracy and stability for spectral and chemical kinetics measurements. The correction is valid from 250 nm to 700 nm, with the option to extend the corrected wavelength region up to 1700 nm.


Multi-mode Detector

The PTI QuantaMaster 400 uses our new generation High Sensitivity Multi-Mode PMT detector Model 914. In the PTI QuantaMaster 400, the 914 is primarily used in digital mode, specially designed for single photon counting - the most sensitive light detection technique. The 914 has additional modes of detection – three analog and one direct – making the detector future ready for other types of operations - like time-resolved phosphorescence with a pulsed lamp or laser using the transient digitizer capability of the ASOC-10 interface. Switching between the digital and analog detection is conveniently done through the PTI FelixGX operating software.

There are many types of PMT tubes available to work with the PTI QuantaMaster 400. The two most commonly used are:

K 170 B

K 170 B
Spectral Response 185 to 680 nm
Photocathode Material Low noise bialkali
Effective Area of PMT 8 x 24 mm
Supply Voltage 1250 V dc
Cathode Sensitivity
Quantum Efficiency at 300 nm (Peak)
Luminous
Radiant 400 nm (Peak)
 
19 % typ.
60 µA/lm typ.
60 mA/W typ.
Anode Sensitivity
Luminous
Radiant 400 nm
 
400 A/lm typ.
4 x 105 A/W typ.
Gain 6.7 x 106 typ.
Anode Dark Current
(After 30minute Storage in the darkness)
0.1 nA

QuantaMaster UV VIS PMT

PMT Spectral Response

K 171 B is optional - cooled housing is recommended due to higher dark count.

K 171 B
Spectral Response 185 to 900 nm
Photocathode Material Multialkali
Effective Area of PMT 8 x 24 mm
Supply Voltage 1250 V dc
Cathode Sensitivity
Quantum Efficiency at 260 nm (Peak)
Luminous
Radiant 400 nm
 
25.4 % typ.
250 µA/lm typ.
74 mA/W typ.
Anode Sensitivity
Luminous
Radiant 400 nm
 
2500 A/lm typ.
7.4 x 105 A/W typ.
Gain 1 x 107 typ.
Anode Dark Current
(After 30minute Storage in the darkness)
3 nA

QuantaMaster UV VIS PMT

PMT Spectral Response

 

For NIR the following PMT tubes are available:

Steady State NIR PMTs

Model QM-600-5509-42 QM-600-H45 QM-600-5509-72 QM-600-H75
Detector type NIR PMT NIR PMT NIR PMT NIR PMT
Application Steady State Steady State Steady State Steady State
Spectral range 300–1400 nm 950–1400 nm 300–1700 nm 950–1700 nm
Cooling method Liquid N2 TE Liquid N2 TE
Operation temperature -80 °C -60 °C -80 °C -60 °C
Minimal measurable lifetime N/A N/A N/A N/A

 

PTI FelixGX Software

PTI QuantaMaster 400 comes with comprehensive PTI FelixGX software for the control of the instrument and accessories, including analytical functions for trace manipulation and spectral and kinetic analysis.

Through the powerful ASOC-10 USB interface, PTI FelixGX provides a full set of data acquisition protocols and controls the hardware for all system configurations and operating modes.

FelixGX controls:

  • Monochromators
  • Triple grating turret
  • Flipping mirrors switching between different light sources and detectors
  • Motorized slits
  • Motorized polarizers
  • Motorized sample holders
  • Temperature control Peltier devices
  • Cryostat
  • Gain control of PMT detector
  • Switching from digital to analog mode
  • External devices such as stopped flow

Additionally, PTI Felix GX also provides some ‘specialty’ calculators:

  • Quantum yield calculator for integrating sphere
  • FRET calculator
  • CIE 1931 and CIE 1976 color coordinates calculator
  • Absorption calculator
  • Single-wall carbon nanotube size calculator

PTI QuantaMaster 400 System Enhancements

QM-400 Phos—Phosphorescence Enhancement

QM-400 Phos Phosphorescence Enhancement adds phosphorescence lifetime measurement capability to an existing fluorometer. It includes a pulsed xenon light source and excitation and emission flipping mirrors. The standard PTI QuantaMaster 400 multimode detector supports this mode of operation.

QM 2nd Em Mono—Tunable Second Emission Detection Channel

The QM 2nd Em Mono adds a second emission channel to a PTI QuantaMaster 400. It includes a second computer controlled emission optimized monochromator with a triple grating turret, an additional multimode PMT detector, additional sample compartment optics for the second emission channel, and a lid-activated shutter.

QM-2nd Em Filter—Second Emission Channel

The QM 2nd Em Filter adds a second emission channel to a PTI QuantaMaster 400. It includes an additional multimode PMT detector coupled directly to the sample compartment. (Wavelength selection is by means of a filter). It can operate in both analog and digital (photon counting) modes. Additional optics for the sample compartment and a lid activated shutter are also included.

Q-80A-40—Fluorescence Microscopy Enhancement

The Q-80A-40 Fluorescence Microscopy add-on adapts an existing spectrofluorometer to a fluorescence microscope using a photometer for detection. Includes a liquid light guide to couple the excitation source of an existing system to a microscope, an excitation mono flipping mirror and a complete photometer using a high sensitivity PMT. Note that a C-mount port and adaptor for the microscope are required but not included.

Q-90A—Fluorescence Microscopy Enhancement

The Q-90A Fluorescence Microscopy add-on adapts an existing spectrofluorometer to a fluorescence microscope using the existing emission monochromator for detection. It includes a liquid light guide to couple the excitation source of an existing system to a microscope and an excitation mono flipping mirror.  For detection, another liquid light guide is mounted to the C-mount adapter of the microscope and transmits the emission signal to the second entrance port of the existing emission monochromator.  Note that a C-mount port and adaptor for the microscope are required but not included.

Q-90B—Fluorescence Microscopy Enhancement

The Q-90B Fluorescence Microscopy add-on adapts an existing spectrofluorometer to a fluorescence microscope using the existing emission monochromator and liquid light guide for detection. It includes a liquid light guide to couple the excitation source of an existing system to a microscope.  and an excitation mono flipping mirror.  A second liquid light guide is mounted to the exit port of a photometer mounted on the C-mount port of the microscope. The emission signal is transmitted back to the second entrance port of the emission monochromator. Flipping mirrors are used to select whether the light is directed from the sample compartment or the microscope. Note that a C-mount port and adaptor for the microscope are required but not included.

QM-LS—LaserStrobe Lifetime Enhancement

The QM-LS Laser Lifetime add-on adds enhanced fluorescence lifetime measurement capability to an existing spectrofluorometer. It includes a nitrogen laser pumped dye laser source for excitation illumination, coupling optics, a stroboscopic detector and monochromator flipping mirror.

QM-LS-NL—LaserStrobe Lifetime Enhancement Using Q Switched Laser for Excitation

The QM-LS-NL Laser Lifetime add-on adds enhanced fluorescence lifetime measurement capability to an existing spectrofluorometer using a Q-switched laser (not included) to provide the excitation illumination. Includes coupling optics, a stroboscopic detector and monochromator flipping mirror.

QM-Strobe—LED Strobe Lifetime Enhancement

The QM-Strobe Lifetime add-on uses a LED light source for excitation. Includes required optics and adaptors, strobe detector and a flipping mirror for the emission monochromator. Many optional LED sources are available.

DeltaTime-DD-PTI—TCSPC lifetime enhancement

DeltaTime-DD-PTI TCSPC lifetime add-on adds TCSPC lifetime capability to an existing spectrofluorometer with DeltaHub timing electronics and cost-effective pulsed DeltaDiodes. Operation is very simple and user friendly since the electronics can be used in the “direct mode” over the entire lifetime range, eliminating tedious recabling. Measure fluorescence lifetimes as short as 25 ps.

DeltaTime-NL-PTI—TCSPC lifetime enhancement

DeltaTime-NL-PTI TCSPC lifetime add-on adds TCSPC lifetime capability to an existing spectrofluorometer with DeltaHub timing electronics and cost-effective pulsed NanoLED LED sources. Operation is very simple and user friendly since the electronics can be used in the “direct mode” over the entire lifetime range, eliminating tedious recabling. Measure fluorescence lifetimes as short as 50 ps.

ULTIMA-PTI—TCSPC lifetime enhancement

ULTIMA-PTI adds TCSPC short lifetime capability with the highest temporal resolution HUB A+ timing electronics. With 400 fs per channel, this is the world's fastest TCSPC system, enabling the user to measure fluorescence lifetimes of < 5 ps when equipped with an MCP detector and Ti:Sapphire laser.

QM-InGaAs—NIR Enhancement

The QM-InGaAs  is a time of order substitution enhancement to a NIR system with InGaAs detection of 500 to 1700 nm. It uses the existing high intensity 75 watt xenon light source, excitation scanning monochromator and sample compartment, but substitutes a high sensitivity TE-cooled InGaAs detector, electronics, lock-in amplifier and chopper for noise suppression, and includes an additional emission 600 groove grating blazed at 1.2 microns. Detection range is from 500 to 1700 nm.

QM-InGaAs NC—NIR Enhancement

The QM-InGaAs NC is a time of order substitution enhancement to a NIR system with InGaAs detection of 500 to 1700 nm. Uses the existing high intensity 75 watt xenon light source, excitation scanning monochromator and sample compartment but substitutes a high sensitivity TE-cooled InGaAs detector, electronics, and includes an additional emission 600 groove grating blazed at 1.2 microns. Because it does not use a chopper and a lock-in it is somewhat less sensitive than the QM-InGaAs, but at half the cost, it is an extremely economical option. Lock-in and chopper can be added at a later date.

QM-InGaAs ER—Extended NIR Enhancement

The QM-InGaAs ER is a time of order substitution enhancement to a NIR system with InGaAs detection of 900 to 2,200 nm. Extended wavelength detector (900 - 2,200 nm) can only be ordered at time of original order as a replacement for the standard NIR detector.

QM-InGaAs MR—Extended NIR Enhancement

The QM-InGaAs MR is a time of order substitution enhancement to a NIR system with InGaAs detection of 700 to 1,900 nm. Extended wavelength detector (700 – 1,900 nm) can only be ordered at time of original order as a replacement for the standard NIR detector.

QM-PbS—Extended NIR Enhancement

The QM-PbS is a time of order substitution enhancement to a NIR system with an extended wavelength PbS detector (1,000 – 3,200 nm) with preamp. Includes grating (300 l/mm blazed at 2000nm) and special NIR lenses. Can only be ordered at time of original order as a replacement for the standard NIR detector.

QM-InSb—Extended NIR Enhancement

The QM-InSb is a time of order substitution enhancement to a NIR system with an extended wavelength liquid N2 cooled InSb detector (1,500 – 5,500 nm) with preamp. Includes grating (150 l/mm blazed at 4000nm) and special NIR lenses. Can only be ordered at time of original order as a replacement of the standard NIR detector.

PTI QuantaMaster 400 System Specifications

Signal to Noise Ratio

20,000:1 or better (350 nm excitation, 5 nm spectral bandpass, 1 s integration time) 

Data Acquisition Rate

1,000,000 points/sec. to 1 point/1000 sec

 

Inputs

 

4 analog (+/- 10 volts) 
4 photon counting (TTL)
1 analog reference channel (+/- 10 volts)
2 TTL

 

Outputs

 

2 analog (+/- 10 volts) 
2 TTL

Emission Range

185 nm to 680 nm (optional to 1,700 nm)

Light Source

High efficiency “ECO” friendly continuous Xenon arc lamp

Excitation Monochromator

Coma-aberration corrected, asymmetrical, excitation optimized, computer-controlled triple grating turret, 300 mm focal length, Czerny-Turner design

Emission Monochromator

Coma-aberration corrected, asymmetrical, emission optimized, computer-controlled triple grating turret, 300 mm focal length, Czerny-Turner design

Excitation Grating

1200 line/mm 300 nm blaze

Emission Grating

1200 line/mm 400 nm blaze

Optional Grating

75 to 2400 line/mm and holographic models available

Minimum Step

0.022 nm

Detection Multimode

Photon Counting, 3 analog (fast, medium, slow response), direct and single shot transient digitizer (SSTD) mode 

System Control

ASOC-10 computer interface with Felix GX spectroscopy software