Fluorescence Spectroscopy

News

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Steady State Solutions

Overview

HORIBA Scientific’s con­focal, universal fiber coupling solution allows any of our steady state and hybrid fluorometers to be easily connect­ed to virtually any upright or inverted microscope. Spectrofluorometric capabilities include the unparalleled precision and sensitivity of Single-Photon Counting and the speed and sensitivity of CCD detection. Similarly, fluorescence spectral mapping capabilities utilize an automated fast scanning X-Y stage to generate rapid spectral maps with micron level spatial resolution. Of course our microscope solutions preserve the microscope's native optical performance and capabilities. For example, they add no optics to the entire emission optical path. Use the high performance cooled fluorescence camera to image your field of interest. Once you identify a region to spectrally analyze, simply switch your filter cube to a beam splitter or an appropriate dichroic, divert the light to the fluorometer, and you are collecting high resolution spectra. Custom solutions are also available to measure in the UV and NIR regions, beyond the normal range of your microscope.

Couple a microscope and:

  • Measure complete spectra of samples as small as 1 μm.
  •  Get spectra from as little as a few molecules of sample.
  •  Perform localized FRET measurements.

Add an automated stage and camera, and:

  • Create complete spatial/spectral maps.
  • Perform repetitive QC characterization of structured samples like photovoltaics. 

Manufactured by HORIBA

Specifications

Specification

Complete Microscope Solution

Interface to Existing Microscope

Objective

Plan achromat x10 and x60, other magnifications available. 6 position turret

User supplied

Confocal pinholes

Kinematic, 6 diameters, 0.1, 0.2, 0.4, 0.6, 0.8, 1 mm

Kinematic, 6 diameters, 0.1, 0.2, 0.4, 0.6, 0.8, 1 mm

Dimensions, Weight

68 cm x 46 cm x 60 cm, 40 kg (BX53)              
90 cm x 50 cm x 75 cm, 65 kg (IX73)

Excitation source: Scopelite 200 (Optional)

Wavelength range

From 350 to 700 nm

From 350 to 700 nm

Illumination Adjustment

5 preset % apertures: 0, 25, 50, 75, 100

5 preset % Apertures: 0, 25, 50, 75, 100

Output Power

7 Watts

7 Watts

Lamp Life

2000 hrs

2000 hrs

Warm Up

2 min

2 min

Long Term Drift

<0.1% RMS

<0.1% RMS

Cooling

Air cooled by fan

Air cooled by fan

Dimensions

20 x 14.5 x 25.4 cm (WxDxH)

20 x 14.5 x 25.4 cm (WxDxH)

Weight

3.4 kg

3.4 kg

Filters

ND filter sliders

6 positions: 0, 0.3, 0.6, 1, 2 and 3 OD

User supplied

Fibers

Multimode slit to round

UV-VIS : 1.5 m length, 250-850 nm wavelength range
NIR : 1.5 m length, 350-2400 nm wavelength range

UV-VIS : 1.5 m length, 250-850 nm wavelength range
NIR : 1.5 m length, 350-2400 nm wavelength range

Fiber Coupling

Excitation

Collimating lens adapters for Olympus

Collimating Lens Adapters for Olympus, Nikon, Leica and Zeiss

Emission

Universal C-mount adapter with pinhole holder

Universal C-mount adapter with pinhole holder

Camera   

Required for mapping option

Required for mapping option

Fluorescence camera

12 bits, 1.4 Mpix, cooled, low noise

12 bits, 1.4 Mpix, cooled, low noise

Motorized stage

Required for mapping option

Required for mapping option

Repeatibility
Travel range
Manual control
Automatic control

<1.0 µm
75 x 50 mm
With joystick
Through FluorEssence software

Suitable stage


Through FluorEssence software

Software/Computer

FluorEssence
PC requirements

Windows® 7, 32- or 64-bit
At least three available USB ports
2 GB RAM
2 GB hard-disk space
DVD-ROM drive
Video resolution of at least 1280x800

Windows® 7, 32- or 64-bit
At least three available USB ports
2 GB RAM
2 GB hard-disk space
DVD-ROM drive
Video resolution of at least 1280x800

Applications

Microscopy Brochure