Fluorescence Spectroscopy

News

  • HORIBA Scientific Presents Young Fluorescence Investigator Award at Biophysical Show. Read more
  • HORIBA Scientific Announces New Ultima TCSPC lifetime system, the next generation of turnkey TCSPC Fluorescence Lifetime systems...Read more
  • HORIBA Scientific announces the acquisition of the Photon Technology International (PTI) Business... Read more
  • HORIBA Scientific Announces New Generation of Turnkey TCSPC Fluorescence Lifetime Systems...Read more
  • Aqualog CDOM Fluorometer Wins Award as one of Best New Instruments in 2011...Read more
  • HORIBA Scientific and NKT Photonics Introduce the World’s First Commercial Integrated Supercontinuum Powered Spectrofluorometers... Read more
  • Aqualog®, the only simultaneous absorbance and fluorescence system, measures CDOM (colored dissolved organic matter) in water 100 times faster than previous fluorescence methods...Read more
  • World's First 100MHz Picosecond Diode Source With USB Interface and Plug-and-Play Interchangeable Heads ...Read more

FluoroMax Series

Overview

The Source

The xenon source that supplies prime UV performance is focused onto the entrance slit of the excitation monochromator with an elliptical mirror. Besides ensuring efficient collection, the reflective surface keeps all wavelengths focused on the slit.

As an option an additional, software-selectable, xenon flash lamp can be fitted to enable phosphorescence studies.

The Slits

The slits themselves are bilaterally, continuously adjustable from the computer in units of bandpass or millimeters. This preserves maximum resolution and instant reproducibility.

The Excitation Monochromator

The excitation monochromator is an aspheric design which ensures that the image of the light diffracted by the grating fits through the slit. The gratings themselves are plane, blazed gratings that avoid the two major disadvantages of the more common concave holographic gratings: poor polarization performance and inadequate imaging during scans that throws away light. The unique wavelength drive scans the grating at speeds as high as 80 nm/s. The grating grooves are blazed to provide maximum light in the UV and visible region.

The Reference Detector

Before the excitation light reaches the sample, a photodiode reference detector monitors the intensity as a function of time and wavelength. The photodiode detector has a wider wavelength response than the older, traditional rhodamine-B quantum counter, and requires no maintenance.

The Sample Chamber

A spacious sample chamber is provided to allow the use of a long list of accessories for special samples, and encourages the user to utilize a variety of sample schemes.

The Emission Monochromator

All the outstanding features of the excitation monochromator are also incorporated into the emission monochromator. Gratings are blazed to provide maximum throughput in the visible region.

The Emission Detector

Emission detector electronics employ photon-counting for the ultimate in low-light-level detection. Photon-counting concentrates on signals that originate from fluorescence emission, ignoring smaller signals originating in the detector tube (PMT). The more common method of analog detection (used by lower performance fluorometers) simply adds noise and signal together, masking weak emissions.

The emission detector housing also contains an integral high-voltage supply which is factory set to provide the signal-to-noise ratio.

Computer Control

The entire control of the FluoroMax originates in your PC with our FluorEssence™ software and is transmitted through a USB link. On start-up, the system automatically calibrates and presents itself for new experiments, or stored routines instantly called from memory.

Accessories

A wide variety of accessories are applications-oriented and most are interchangeable with the FluoroMax system.

Features

  • Water Raman signal-to-noise ratio: 16000:1 (6000:1 FSD)
  • Photon Counting for Ultimate Sensitivity
  • Fast Scanning capability - up to 80 nm/second
  • Powerful FluorEssence™ software for Windows®
  • Method Files to recall complete experiment parameters
  • Front Surface Detection Accessory
  • Fluorescence Microscope Accessory
  • Polarization and Anisotropy Accessory
  • Temperature Controller Accessory (Circulating bath or Peltier)
  • MicroMax™ Plate Reader Accessory
  • Trigger Accessory
  • Stopped Flow Accessory
  • Autotitrator Accessory
  • Remote Measurement Capability using optical fiber Accessory
  • Time-correlated single-photon counting (TCSPC) accessory for lifetime determinations
  • Phosphorimeter accessory for long-lived luminescence determinations

Manufactured by HORIBA Scientific

Specifications

Lamp

Vertically mounted, CW, 150 W Ozone-free xenon arc lamp

Gratings

1200 groove/mm blazed at 330 nm (excitation) and 500 nm (emission), plane ruled

Automatic self-calibration of all wavelength drives and slits

Monochromators

All reflective optics, Czerny-Turner spectrometers

Detectors

Emission: R928P photon counting PMT (185-850 nm) and reference photodiode for monitoring lamp output

Water Raman S/N

6,000:1 (FSD method) 16,000:1 (RMS method) See Signal-to-noise ratio

Slits

Continuously variable from 0 to 30 nm

Accuracy

0.5 nm

Repeatability

0.1 nm

Minimum step

0.0525 nm

Integration time

0.001 to 160 sec

Software

FluorEssence

Spectral Correction Factors

Included

OpenFluor Webinar

Support Documents

Brochure