Application
OCHRAKING
Overview
OCHRAKING, is an IAC which effectively cleans up Ochratoxin.
Features
High organic solvent-tolerance– efficient test and pretreatment
- Cleans up Ochratoxin with only a small quantity of extract.
- Saves significant pretreatment time and increases the analysis efficiency.
- Purifies Ochratoxin A and B,
- Accepts ammonium acetate solution and purified water for washing columns.
OCHRAKING improves the efficiency of sample clean up.
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Its high organic solvent tolerance enables rapid clean up with only a small quantity of sample solution.
Recovery test/wheat
Ochratoxin in wheat is analyzed by extraction with 60% acetonitrile and diluting the extract with PBS to 30% for cleaning up Ochratoxin A and B. When only analyzing Ochratoxin A, acetonitrile concentration of up to 48% is used.
Recovery test/red wine
■Dilution of sample
HPLC analysis conditions
- Column: ODS (particle diameter 5 μm), inner diameter 4.6 mm, length 150 mm
- Temperature: 45 °C
- Mobile phase: acetonitril, water, acetic acid (55:43:2)
- Mobile phase: 1.0 mL/min.
- Detection wavelength: excitation wavelength 333 nm, fluorescence wavelength 460 nm
- Injection volume: 100 μL
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Manufactured by HORIBA
Specifications
Product name | OCHRAKING |
---|---|
Type | AC02-25 |
Use | Pretreatment column for analysing Ochratoxin in foods |
Recovery ratio | more than 80% in each Ochratoxin A and B when 10mL of 50%methanol solution containing each 5ng of Ochratoxin A and B is added to the column. |
Shelf-time | 12 months after production at 2 ‐ 8℃. |