Bioprocessing

In bioprocessing the online and offline monitoring of the bioreactor condition is of prime importance to increase the efficiency of protein production. One of the biggest challenges is to minimize contaminants that can affect the efficacy of the protein drug downstream. HORIBA Scientific’s Raman, Fluorescence (A-TEEM technology) and SPRi are ideal analytical instruments to monitor cells in real time and optimize yields.

Fluorescence spectroscopy is widely used in a variety of industries including biological sciences. Two dimensional (2D) fluorescence excitation-emission matrix (EEM) spectroscopy has been described in the literature for over 30 years to characterize or “fingerprint” biological samples.

Determination of pH as Quality Control Test in Culture Media-01

We have measured solutions of lysozyme under conditions known to effect its physical state in order to investigate the potential of Raman spectroscopy as a non-invasive and label-free tool to assess protein formulation stability. Results from this study identified specific Raman signature bands in this protein that can be used to identify individual amino acid residues that are reflect structural changes in proteins.

The use of low molecular weight molecules in the industries of drug discovery and agri-food has become increasingly important over the course of a century of drug research and science development in agriculture and in pharmaceutical field.

Monitoring Cell Culture Media Variability using A-TEEM Spectroscopy

With the rise of protein production using mammalian cell culture, it has become increasingly important to control the quality of the cell culture media for use in production processes. Cell culture media are usually prepared as aqueous solutions and should provide everything a cell line needs for optimal cell growth as well as product yield and quality.

Holistic Analysis of Mammalian Cell Proliferation using Fluorescence Spectroscopy

Monitoring culture media conditions is highly important for industries in order to improve cell proliferation for applications such as regenerative medicines and protein synthesis.