Veloci BioPharma Analyzer

A-TEEM Spectroscopy

The Veloci A-TEEM BioPharma Analyzer contains innovative, column-free molecular fingerprinting technology designed for the biopharma and pharma industries. By integrating the selectivity of chromatography with the benefits of optical spectroscopy, Veloci offers a fast, simple, and cost-effective method for comprehensive component analysis in various industrial QC/QA life science applications. Unlike traditional methods such as chromatography and mass spectrometry, Veloci’s A-TEEM™ Molecular Fingerprinting technology provides component analysis without time consuming and slow separations. This allows simplified workflows and drastically reduces ownership costs. This versatile tool requires no sample preparation and is suitable for a wide range of applications, including monoclonal antibody discrimination, cell media monitoring, vaccine characterization, protein stability analysis, and AAV quantification. The Veloci A-TEEM BioPharma Analyzer enables label-free intrinsic fluorescence analysis, offering high accuracy and efficiency in research, production and QC/QA environments.

Segment: Scientific

Abteilung: Fluorescence Spectroscopy

Produktionsfirma: HORIBA Scientific

Product Videos

Veloci A-TEEM BioPharma Analyzer

A-TEEM Direktor Software

Key Benefits

Selectivity of chromatography, but using spectroscopy.
Superior sensitivity compared to HPLC/MS and other spectroscopic techniques like absorbance, FTIR, and Raman.
Hassle-free operation with no columns, mobile phase, or sample preparation needed. The lack of a mobile phase drastically reduces solvent usage and eliminates the cost of proper disposal.
Cost-effective and user-friendly: Suitable for both research labs and production processes

Applications

Monoclonal Antibodies (mAb)
AAVs (Adeno-Associated Viruses)
Vaccines
Protein Stability and Aggregation
Antibody-Drug Conjugates
Cell Media Monitoring and Bioreactor Monitoring
Exosomes

EzSpec Software

EzSpec allows users to see recent methods and data, as well as personal favorites. Click here for more on EzSpec.

EzPAT Server

EzPAT server enables integration of Veloci A-TEEM methods with PAT Enterprise software, providing secure instrument control through OPC UA protocols.

EzSpec-P11

EzSpec-P11 enhances HORIBA Veloci and Aqualog UV-800 systems with advanced data integrity, audit trail, electronic records, and electronic signature capabilities, helping regulated laboratories support GMP workflows and compliance with FDA 21 CFR Part 11 requirements.

A-TEEM Compliance Package

HORIBA’s A-TEEM Compliance Package provides solutions for record-keeping, method validation, and instrument verification to meet regulatory requirements.

Download the Veloci BioPharma Analyzer brochure to learn more

Veloci Biopharma Analyzer
Detection Limit	Parts per billion (sample and wavelength dependent)
Excitation/Absorbance Wavelength Range	200 to 800 nm
Fluorescence Emission Wavelength Range	250 to 800 nm
Wavelength Accuracy	+/- 1 nm
EEM/A-TEEM Acquisition Rate	As fast as 60 seconds (sample and wavelength dependent)
Optional Accessories	Remote fiber optic probe, Autosampler with flow cell
Minimum Sample Volume	70 microliters
Sample Handling, Internal	Cuvette and solid sample holders
Sample Handling, External	Autosampler (96 sample maximum)
Light Source	Xenon arc lamp
Validation	Complies with US Pharmacopia
Dimensions (W x D x H)	25.3 in x 18 in x 14.5 in; 642.6 mm x 457.2 mm x 368.3 mm
Weight	32.72 kg (72 lbs.)

Characterizing Monoclonal Antibody Purification with 2D Fluorescence Spectroscopy

Fluorescence spectroscopy is widely used in various industries, including life sciences. Two-dimensional (2D) fluorescence excitation-emission matrix (EEM) spectroscopy has been utilized for over 30 years to characterize or “fingerprint” biological samples.

Monitoring Cell Culture Media Variability Using the Veloci and A-TEEM Molecular Fingerprinting

Cell culture media for bioreactors are commonly prepared as aqueous solutions that provide the essential nutrients and conditions required for optimal cell growth, product yield, and quality. Even subtle variations in media composition can significantly impact cell growth rates and yield. Consequently, the identification and analysis of cell culture media are critical for maintaining consistency and optimizing performance.

Monitoring Culture Medium Conditions During Cell Proliferation Using the Veloci BioPharma Analyzer

Monitoring culture media conditions is critical for optimizing cell growth in the Biopharma industry. The health of cells has drastic impacts on the yield and quality of APIs in regenerative medicine, monoclonal antibody production (mAB), protein synthesis, and many more applications.

Simultaneous A-TEEM and UV-Vis Analysis for Insulin Structure and Stability Assessment

Stability and aggregation of insulin are studied using simultaneous fluorescence excitation-emission matrices (EEMs) and UV-Vis absorbance spectroscopy. Insulin is a protein-hormone produced by the pancreas and is necessary for basic metabolic processes. Commercial insulin therapeutics generally fall into two categories: short-acting and long-acting insulin. The difference between some short-acting and long-acting insulin lies, in some cases, in only one to three residues in the protein sequence.

Utilizing A-TEEM Technology for In-Process Monitoring of Monoclonal Antibody Production

In this paper, HORIBA presents the Centre for Process Innovation (CPI) evaluation work of A-TEEM as a suitable technique to analyse six in-process purification samples of monoclonal antibodies—an initial step toward implementing this technique as a PAT solution in biologics manufacturing.

Bioprocess Monitoring - Cell Growth Assessment using the Veloci™ BioPharma Analyzer

Accurate monitoring of bioprocesses is essential, as small variations in culture conditions can impact productivity, product quality, and process consistency. One key challenge lies in analyzing the complex, dynamic environment of bioreactors, where media composition, metabolic byproducts, and matrix effects introduce variability. As the industry shifts toward intensified and continuous processing, there's growing demand for rapid, real-time analytical tools that operate under sterile, GMP-compatible conditions. A-TEEM™ technology meets these requirements with its high sensitivity, molecular selectivity, and real-time data output, providing actionable insights for optimizing cell growth and overall bioprocess performance.

Cell Culture Media Analysis: Qualitative and Quantitative Analysis using the Veloci™ BioPharma Analyzer

This study demonstrates the potential of A-TEEM™ spectroscopy as a rapid and reliable tool for evaluating lot-to-lot variability in complex cell culture media. By leveraging the distinct spectral fingerprints of aromatic amino acids and vitamins, A-TEEM enables sensitive tracking of compositional differences without the need for extensive sample preparation or lengthy separations.

Non-Invasive Analysis of Historical Chinese Textile Dyes using EEM Fluorescence Spectroscopy

This application note describes a breakthrough non-invasive analytical approach using Excitation-Emission Matrix (EEM) fluorescence spectroscopy for identifying natural dyes in Chinese royal textiles from the Qing dynasty (1616-1912 CE).

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Veloci Biopharma Brochure

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A-TEEM Direktor Brochure