ViewSizer 1000

Multi-Variable Pulse Nanoparticle Tracking Analysis (NTA)

The ViewSizer™ 1000 combines the advantages of multispectral NTA with the simplicity of a single laser system by utilizing multi-variable pulse nanoparticle tracking analysis (MVP NTA).

Achieving efficient, repeatable, reproducible, and accurate measurements of nanoparticles size distribution and concentration remains a challenge for complex samples, such as extracellular vesicles (EVs), ultra-fine bubbles, liposomes, viruses, proteins, and colloids. Light scattering techniques, such as laser diffraction and dynamic light scattering (DLS) yield fast and accurate average size, but details are lost due to their ensemble nature.

Nanoparticle tracking analysis (NTA) overcomes this limitation by analyzing individual particles. The ViewSizer 1000, driven by MVP NTA, offers a new patented approach that overcomes these issues without the complexity of multispectral NTA.

Segment: Scientific
Výrobní společnost: HORIBA Instruments Incorporated

Key Features

  • Collect accurate data for all particles in a sample, even for wide-size distributions, using patented multi-variable pulse technology (MVP NTA).
  • Excellent repeatability and reproducibility due to stable optics, temperature control, and effective, unbiased mixing.
  • Access larger particle sizes with high spatial resolution NTA for slowly moving particles, including sedimentation analysis.
  • Prevent cross-contamination and increase throughput by using easily washed and replaced cuvettes.
  • Flexible analysis with user access to laser intensity, camera gain, pulse duration, and frame rate settings.
  • Recommended and robust default method settings for many applications are included with the system.
  • Solvent resistant (wetted materials: glass/quartz, fluoropolymers, and anodized aluminum, glass/fluoropolymer cells also available) 
  • Reduced sample volume due to no feed tubing or pump required.

Introducing Multi-Variable Pulse NTA

Using next generation hardware and software, a series of varying pulse widths are used in place of varying laser powers. Each pulse width has its own independent sequence of video frames. Long pulse frames have good data on small particles, while short pulse frames have good data on large particles. Position as a function of time, that is particle movement, is then analyzed by the Stokes-Einstein relationship to extract particle size for each particle in the sample.

Since sample volume is well known, along with the number of particles images, particle number concentration is determined without any further information required. Random mixing with a stir bar (not slow flow through a tube) ensures random selection of particles for each video without the biases of a flow-based system. The instrument automatically sums the particles identified in each video and divides by the measurement volume to calculate the sample concentration.

Thus, detailed particle size distribution and concentration is rapidly measured in a single system.

Advanced Software

The ViewSizer software is designed for intuitive workflow. Data collection can be initiated with just a few clicks, with results generated in multiple exportable formats for downstream analysis. The user interface includes real-time visualization of all particles – providing valuable insights into sample behavior and data quality.

    Measurement Range

    10 nm to 15 μm

    Sample Volume

    100 microliters to 2.5 mL

    Sample Concentration
    (Sample dependent)

    1E5 to 1E9 p/mL

    Dimensions

    55 cm (W) x 66 cm (D) x 35 cm (H)

    Operational Environment

    		15°C to 30°C with <85% RH 
    (Dry gas purge feature allows operation below dew point)
    Electrical100 to 240 VAC, 47-63 Hz inclusive, <450 Watts
    Class 1 Laser21 CFR Ch. I part 1040
    Investigating Polydispersity Limits with Multi-Variable Pulse Nanoparticle Tracking Analysis
    Investigating Polydispersity Limits with Multi-Variable Pulse Nanoparticle Tracking Analysis
    The ability to measure a sample across a broad range of sizes can be challenging for NTA instruments in general. Small size particles need high energy laser scattering due to the exponential decay in Mie scattering below 100 nm, while large size particles need low energy laser scattering to avoid excessive laser diffraction patterns and to better visualize and track the particles. The ViewSizer 1000 helps address this.
    Zobrazit více
    Investigating Multi-Modal Peak Resolution Capabilities with Nanoparticle Tracking Analysis
    Investigating Multi-Modal Peak Resolution Capabilities with Nanoparticle Tracking Analysis
    One advantage that the nanoparticle tracking analysis technique has over other particle sizing techniques is the ability to resolve multiple peaks in a sample, allowing for multi-modal size and concentration analysis of each population in a sample. Other techniques, like laser diffraction or dynamic light scattering, will often struggle when the sizes of different populations in a sample start to get too close, and are instead measured as a single peak.
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    Repeatability Analysis for Size and Concentration using Nanoparticle Tracking Analysis
    Repeatability Analysis for Size and Concentration using Nanoparticle Tracking Analysis
    Repeatability is an important factor that is often overlooked with NTA measurements, but it is good practice to always check repeatability when performing measurements as it not only checks that the instrument is giving accurate and repeatable results, it also checks that the sample is stable at that dilution or measurement condition. In this application note, Fuso PL-71 colloidal silica was measured fifteen times on the same sampling to evaluate instrument repeatability and sample stability.
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    Sedimentation Analysis of 12 Micron Particles by PTA with the ViewSizer™ 1000
    Sedimentation Analysis of 12 Micron Particles by PTA with the ViewSizer™ 1000
    For a normal nanoparticle tracking analysis measurement, the particles are generally smaller than 1 micron and show random Brownian motion rather than settling motion. However, when particles start to get larger than 1 micron, they tend to show more settling motion than Brownian motion, requiring a different equation to size the particles. The exact size at which the particle starts to show more settling motion than Brownian motion depends on the particle’s density, but when settling is observed, the particle size can be calculated using the Stokes’ Law, which relates the speed of settling to the particle size.
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    Silicone Emulsion Size and Concentration Measurement with Nanoparticle Tracking Analysis
    Silicone Emulsion Size and Concentration Measurement with Nanoparticle Tracking Analysis
    Silicone emulsions are used in a variety of applications like polishing, lubricating, and sealing. Measuring the particle size of silicone emulsions is important, as smaller size emulsions are generally clearer, more stable, and penetrate deeper into substrates. Using a technique like nanoparticle tracking analysis (NTA) can be useful in determining the efficiency of the emulsification process by having a higher resolution to accurately characterize the width and tails of the distribution, especially on the larger side.
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    OLED Polymer Fluorescent Measurement with Nanoparticle Tracking Analysis
    OLED Polymer Fluorescent Measurement with Nanoparticle Tracking Analysis
    LEDs are light-emitting diodes with an organic layer designed to emit certain wavelengths of light in response to current, and are used in a variety of applications like high-end TV screens, computer monitors, and smartphones. Of the possible compounds to make up the organic layer, fluorene copolymers are an important organic compound with high photoluminescence and blue light emission. In addition to being used in OLEDs, fluorene copolymers can also be used in solar cells and in bioimaging applications.
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    Colloidal Silica Size and Concentration with Nanoparticle Tracking Analysis
    Colloidal Silica Size and Concentration with Nanoparticle Tracking Analysis
    In this application note, Fuso PL1 colloidal silicas were measured using the ViewSizer 1000 Nanoparticle Tracking Analyzer to investigate its ability to visualize small particles with lower scattering properties, and to check the resolution capabilities in identifying trace oversize particles.
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    Nanobubble Size and Concentration Measurement with Nanoparticle Tracking Analysis
    Nanobubble Size and Concentration Measurement with Nanoparticle Tracking Analysis
    In this application note, a nanobubble sample was measured with the ViewSizer 1000 Nanoparticle Tracking Analyzer.
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    mRNA-LNP Vaccine Size and Concentration Measurement with Nanoparticle Tracking Analysis
    mRNA-LNP Vaccine Size and Concentration Measurement with Nanoparticle Tracking Analysis
    Vaccines are essential in the eradication of many of the world’s diseases and continue to innovate with new mRNA vaccines being designed. In the case of mRNA vaccines, lipid nanoparticles (LNPs) are being used as the vector to transport the mRNA safely throughout the body. Knowing the particle size and concentration of these LNPs is essential in tracking the biodistribution and cellular uptake of the vaccine in the body. In this application note, an mRNA-LNP vaccine sample was measured with the ViewSizer 1000.
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    Evaluating Particle Concentration Linearity Using the ViewSizer™ Multi-energy Nanoparticle Tracking Analysis (NTA)
    Evaluating Particle Concentration Linearity Using the ViewSizer™ Multi-energy Nanoparticle Tracking Analysis (NTA)
    Robust concentration measurement is an important performance metric for nanoparticle tracking analysis. Commercially available concentration standards validated by orthogonal techniques, such as TEM or sp-ICP-MS, can be used to assess concentration accuracy. However, not every laboratory has access to reference material, or requires this level of accuracy for routine verification. In this study, NIST-traceable polystyrene latex (PSL) standards were used to evaluate dilution linearity...
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    Size: 1.73 MB
    ViewSizer 1000 Brochure

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