What is the interest of SPR/SPRi?

SPR is a label-free technology. SPR doesn’t require either labeling of the samples to inject or a revelation step with a secondary labeled molecule.

The interest of SPR, in comparison with more conventional label-based technologies like fluorescence, lies in the real time measurement of the interaction.

Real time measurement gives access to the evaluation of the kinetic parameters of the interaction (association rate, dissociation rate and affinity/avidity)

SPRi is adding an imaging capacity to SPR. This feature gives access to the follow-up from several dozen to several hundred interactions in parallel (multiplexing).

Up to date, SPRi is the only commercially available technology regrouping label-free, real time measurement and imaging of up to several hundred interactions in parallel with the possibility of affinity calculation for each of these interactions.