- Reference: SPRi3
- Dates:
- March 12-15, 2018
- September 10-13, 2018
- Who should attend: XelPleX or OpenPlex users People wishing to acquire an XelPleX or an OpenPlex
For this training the attendees should be from the same team - Duration: 4 days (From 9 am to 5:30pm)
- Learn how to optimize experimental conditions in function of interactions to analyze
- Learn how to spot the biochip with the spotter defined by the user
- Master the use of the SPRi system, defined beforehand by the user
- Learn how to analyze data
Day 1
- Which biochip to choose according to the molecules to immobilize?
- How to optimize spotting (concentrations, buffers, reference, ...)?
- The important experimental parameters to be tested for SPRi (pH, running buffer, temperature,...)
- Advice to be applied during the experiment (duration of kinetics, flow rate, regeneration, ...)
- Establishment of an experimental protocol
Day 2
- Choose suitable surface chemistry for immobilized ligands
- Perform spotting based on the previously defined protocol
- Advice about the maintenance of the instrument
Day 3
- Carry out the SPRi experiment as described in the protocol
- Master the use of the chosen SPRi device (XelPleX or OpenPleX)
Day 4
- Reminder on interaction kinetics
- Software use
- Data analysis
