Training

  • Reference: SPRi2
  • Dates:

    • May 14-17, 2018
    • October 22-25, 2018

  • Who should attend: XelPLeX users or people wishing to acquire an XelPleX
  • Duration: 4 days (From 9:00 am to 5:30 pm)

  • Learn how to optimize the experimental conditions according to the interaction to be analyzed
  • Learn how to make a biochip spotting
  • Master the use of XelPleX
  • Learn how to analyze a kinetic data

Day 1

  • Introduction to kinetics of interaction: What is a «good» kinetic?
  • Which biochip to choose according to the molecules to immobilize?
  • How to optimize spotting (concentrations, buffers, reference, ...)?
  • The important experimental parameters to be tested in SPRi (pH, running buffer, temperature,...)
  • Advice to be applied during the realization of the experiment (duration of kinetics, flow rate, regeneration, ...)
  • Case studies

Day 2

  • Choose the right surface chemistry as a function of immobilized ligands
  • Optimize experimental immobilization conditions (spotting buffer, pH, ligand concentration, duration of needle rinses / dryings, etc.)
  • *Master the use of the spotter (master the different  steps to program a spotting matrix)
  • Performing spottings

Day 3

  • Optimize the experimental conditions according to the interaction to be analyzed
  • Master the use of XelPleX
  • Two models of interactions will be studied:

    • Study of a first interaction (protein / protein)
    • Study of a second interaction (DNA / DNA)

Day 4

  • Reminder on interaction kinetics
  • Use of EzFit software. Analysis of different interactions (Ac / Ac, DNA / DNA, Protein / small molecule, ...)
  • Carrying out analysis in «classical» mode (injection of several concentrations of the analyte) and in «single-injection» mode (injection of a single concentration of the analyte)

*The choice of the spotter used must be defined when registering