
Training
SPRi - XelPleX
- Reference: SPRi2
- Dates:
- May 14-17, 2018
- October 22-25, 2018
- Who should attend: XelPLeX users or people wishing to acquire an XelPleX
- Duration: 4 days (From 9:00 am to 5:30 pm)
- Learn how to optimize the experimental conditions according to the interaction to be analyzed
- Learn how to make a biochip spotting
- Master the use of XelPleX
- Learn how to analyze a kinetic data
Day 1
- Introduction to kinetics of interaction: What is a «good» kinetic?
- Which biochip to choose according to the molecules to immobilize?
- How to optimize spotting (concentrations, buffers, reference, ...)?
- The important experimental parameters to be tested in SPRi (pH, running buffer, temperature,...)
- Advice to be applied during the realization of the experiment (duration of kinetics, flow rate, regeneration, ...)
- Case studies
Day 2
- Choose the right surface chemistry as a function of immobilized ligands
- Optimize experimental immobilization conditions (spotting buffer, pH, ligand concentration, duration of needle rinses / dryings, etc.)
- *Master the use of the spotter (master the different steps to program a spotting matrix)
- Performing spottings
Day 3
- Optimize the experimental conditions according to the interaction to be analyzed
- Master the use of XelPleX
- Two models of interactions will be studied:
- Study of a first interaction (protein / protein)
- Study of a second interaction (DNA / DNA)
Day 4
- Reminder on interaction kinetics
- Use of EzFit software. Analysis of different interactions (Ac / Ac, DNA / DNA, Protein / small molecule, ...)
- Carrying out analysis in «classical» mode (injection of several concentrations of the analyte) and in «single-injection» mode (injection of a single concentration of the analyte)
*The choice of the spotter used must be defined when registering
- Telephone: + 33 (0) 1 69 74 72 00
- Fax: + 33 (0) 1 69 09 07 21
- Email: training.hfr@horiba.com