Rapica

Rapid Microorganism Detection System

Detection at 1 microbiological cell level, on the same day

The time required for microbiological testing has been drastically reduced, as the filtration and concentration of a sample only takes 30 minutes (for 100 mL WFI), while the luminescent reaction measurement thereafter takes approximately 2.5 hours. Importantly, it is possible to achieve same day detection at the single microbe level. HORIBA also provides full support for evaluation testing and method development.

Division: Rapid Microbial Test
Manufacturing Company: HORIBA Advanced Techno Co., Ltd.

Measurement Flow of the High-Sensitivity ATP bioluminescense

The high-sensitivity ATP bioluminescense uses five different reagents for measurement. The reagent injection process is automated, saving labor and reducing the risk of contamination through human interaction.

Measurements Independent of the Type of Microbe

Since all organisms utilize ATP, it can be measured regardless of whether the sample contains bacteria, mold, or yeast. With the germination process included, bacteria that are in the spore state can also be measured.

Only ATP from Viable Microbes is Measured

As the ATP elimination process eliminates free ATP and ATP from non-viable microbes, only viable microbes are measured.

Rapica measurement completed in 2.5 hours.

With Rapica, testing time is greatly reduced when compared to conventional culture methods, with results available on the same day in approximately 2.5 hours.

Measurement Unit

Model NameML-100
Measuring PrincipleHigh-sensitivity ATP bioluminescense
Number of SamplesMaximum 24 samples
Minimum Sample Size100 µL
Measurement ParametersAmount of ATP in viable cells (unit: amol = 10-18 mol)
Measurement Range0ー1000 amol (Calibration with 0 and 1000 amol standard solutions for each measurement)
Measurement Processing TimeApprox. 2.5 hours (excluding filtration and other pretreatment time)
Guaranteed
Performance
RepeatabilityCV≦5.0% (1000 amol standard solution)
LinearityR2: 0.99 or more, slope: 0.90-1.10 (ATP standard solution automatically prepared for multiple concentrations within the measurement range)
Limit of detection1.0 amol
Air CleanlinessJIS B 9920 Class 5, ISO 5, US209E Class 100 recommended
External Dimensions500(W)×500(D)×500(H) mm (excluding protruding parts)
Maximum height 870 mm
Weight45 kg
Power SpecificationsAC 100-240V, 250VA, 50/60Hz

Filtration Unit

Model NameML-01-FU
Number of SamplesMaximum 12 samples
Sample Volume100 mL per port
Filtration TimeApprox. 30 minutes (WFI 100 mL)
External Dimensions420(W)× 340(D)×380(H) mm
Weight20 kg
Power SpecificationsJP: AC 100V, 175VA, 50/60Hz
EU, US: AC 220-240V, 175VA, 50/60 Hz

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