Applications

Leaf Fluorescence: Macroscale FLIM and Microscopic FLIM of Mobile Chloroplasts

Photosynthesis is a key component of life on earth. Studying the steps involved in the photosynthetic process from the absorption of light through to its conversion to energy and gas is essential in many aspects. These range from agricultural to fundamental biology and the renewable energy area. The leaf can be thought of as a scaffold for supporting the chloroplasts containing the photosynthetic reaction centers. Fluorescence is particularly apt for the study of photosynthesis and fluorescence lifetime imaging (FLIM) is particularly suited as it provides spatial information. The FLIMera™ widefield TCSPC camera can be equipped with a lens and filter enabling the whole of the leaf to be rapidly imaged, providing macro FLIM capability. More typically, when attached to a microscope, its ability to collect over 24,000 fluorescence decays simultaneously at rates up to ~12.5 kfps, allows it to capture lifetime data from moving samples. This is demonstrated in this note observing whole leaf fluorescence.

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Leaf Fluorescence: Macroscale FLIM and Microscopic FLIM of Mobile Chloroplasts
Studying the steps involved in the photosynthetic process from the absorption of light through to its conversion to energy and gas is essential in many aspects. These range from agricultural to fundamental biology and the renewable energy area. Fluorescence is particularly apt for the study of photosynthesis and fluorescence lifetime imaging (FLIM) is particularly suited as it provides spatial information.
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