PLQY Integrating Sphere

QuantaPhi-2 features a large, 121 mm internal diameter Spectralon® integrating sphere with excellent reflectivity from 250 to 2500 nm. This is an internal slide-in, tray-mounted integrating sphere with two excitation, and two emission ports.

Each port has a Spectralon plug with sphere-matching curvature for when openings are not in use. The QuantaPhi-2 features a unique bottom loading sample tray for solids or powders, ensuring that any sample spills are limited to the small, replaceable Spectralon cup. The sample cup is 1 cm in diameter and 3 mm in depth, with a quartz coverslip for powder containment. This bottom loading tray can save a tremendous amount of time and money, as the prevention of sphere contamination is the number one priority when using an integrating sphere.

The QuantaPhi-2 also includes a center-mounted 10 mm cuvette sample holder for PLQY studies of samples in solution.

Upconversion PLQY and Laser-excited PLQY

This PLQY sample tray accessory also allows for direct mounting of DPSS lasers on the front of the sample tray for upconversion PLQY or laser-excited PLQY. Contact HORIBA for a list of available DPSS lasers.

Segmento: Scientific
Fabricante: HORIBA Scientific

Direct Quantum Yield Measurements of Unknown Luminescent Samples

Quantum yield is one of the most important parameters that characterize photoluminescence of materials. Photoluminescence Quantum Yield (PLQY) measurements are critical for a broad range of applications, including new material development, photovoltaics and the development of new fluorescence probes. With thousands of PLQY citations  around the world, HORIBA has long been recognized for its superior quality of PLQY performance for the most demanding applications.

The QuantaPhi-2 is a new internal photoluminescence quantum yield (PLQY) and CIE measurement accessory for compatible HORIBA fluorescence spectrometers. Compatible HORIBA fluorescence systems include the following:


  • Fluorolog-QM modular research spectrofluorometer
  • Nanolog modular research spectrofluorometer
  • FluoroMax Plus bench top research spectrofluorometer


This sample tray accessory includes an all-reflective sphere into which a sample is placed. The measurement of the sample, and of a non-fluorescent blank, allows for the direct measurement of the quantum yield of a solid, powder or solution sample.

Combined with our highest sensitivity and flexible spectrofluorometers, and their simple-to-use, dedicated QY and colorimetry software, the QuantaPhi-2 provides a high quality, simple and absolute PLQY solution. The quantum yield of a molecule or material is defined as the number of photons emitted as a fraction of the number of photons absorbed. This characteristic property of a fluorophore or fluorescent molecule, is very important for understanding molecular behavior and optimization for many key materials.

Materials and Applications for which PLQY are Important Parameters

  • Photovoltaics (PV) and solar cells
  • Novel nanomaterials
  • Nanoparticles
  • Quantum dots
  • Graphene/single walled carbon nanotubes
  • New fluorescent probes
  • Biomarkers and biosensors
  • Lighting and display materials (OLED, LED and phosphors)
  • Thin films and coatings
  • Light emitting devices
  • Cured/doped polymers, gels, hydrogels
  • Paints, coatings,
  • Rare earth materials


Characterize Light-emitting Devices

Electroluminescent quantum yield (ELQY) measurements of light-emitting devices, such as LEDs, OLEDs, and other luminescent sources, are fully accommodated by the QuantaPhi-2. Electrical connections to the device are facilitated by a dedicated wiring port in the bottom loading drawer, allowing a customer to feed wires to their custom modified sample cup holder. Shown below are the CIE values and total luminous intensity plots of a simple LED measured using the QuantaPhi-2.

HORIBA FluorEssence software interface for the Fluoromax and Nanolog spectrofluorometers.

Software That Makes PLQY Simple and Accurate

The QuantaPhi-2 integrating sphere accessory is compatible with a number of HORIBA fluorescence spectrometers, and all of these systems have a PLQY App, that simplifies the process of acquiring an accurate QY value for an unknown sample.

FelixFL software on the Fluorolog-QM modular research spectrofluorometer incorporates a quantum yield calculator which, when coupled with an integrating sphere, allows you to calculate the quantum yield with ease.

QuantaPhi-2 Hardware Specifications


Sphere material*Spectralon®
Reflective wavelength range> 95%, 250 to 2,500 nm
Sphere inner diameter121 mm
Port fraction (liquid mode)


Port fraction (solid mode)


Powder sample cup13 mm diameter x 3 mm depth with 15 mm quartz coverslip
Liquid sample holder1 cm path quartz cuvettes (2 to 4 mL)
Gas purge connectors1/4" ID tubing
PLQY sample tray size152 x 183 x 190 mm (W x D x H)
PLQY sample tray weight4.7 kg


QuantaPhi-2 Performance Specifications

PLQY accuracy (Quinine Sulfate, Accepted value QY = 55 ± 10%)55.1 ± 0.8
PLQY accuracy (Rhodamine, Accepted value QY = 93 ± 10%)93.2 ± 0.5
Precision, Relative Standard Deviation (RSD)2%
PLQY wavelength range with Fluorolog-QM250 to 2,200 nm (detector dependent)
PLQY wavelength range with FluoroMax Plus250 to 1900 nm (detector dependent)
PLQY wavelength range with Nanolog250 to 2,200 nm (detector dependent)


*Spectralon is a registered trademark of Labsphere, Inc.

Request for Information

Do you have any questions or requests? Use this form to contact our specialists. * These fields are mandatory.

Related products

Aqualog - Water Treatment Plant Analyzer
More Aqualog - Water Treatment Plant Analyzer

Automated Organic Analysis & Early Warning Sentinel

Aqualog Datastream
More Aqualog Datastream

For Instant Water Quality Reports

More CFD-2G


More DAS6

Decay Analysis Software

More DeltaDiode

Pulsed Laser and LED Light Sources

More DeltaFlex

TCSPC Lifetime Fluorometer

More DeltaPro

TCSPC Lifetime Fluorometer

More DeltaTime

TCSPC Lifetime Kit

More Duetta

Fluorescence and Absorbance Spectrometer

More FLIMera

Imaging camera for dynamic FLIM studies at real time video rates

FluoroHub A+
More FluoroHub A+

TCSPC Lifetime Controller

More Fluorolog-QM

Modular Research Fluorometer for Lifetime and Steady State Measurements

More FluoroMax

Steady State and Lifetime Benchtop Spectrofluorometer

Hybrid Picosecond Photon Detector (HPPD) Series
More Hybrid Picosecond Photon Detector (HPPD) Series

HORIBA’s latest development in TCSPC detector technology

Microsense microliter fluorescence measurement solution
More Microsense microliter fluorescence measurement solution

Easy to deposit, easy to recover, easy to clean.

More NanoLED

Pulsed Laser and LED Light Sources

More Nanolog

Steady State and Lifetime Nanotechnology EEM Spectrofluorometer

More Nanosizer

For Single‐Walled Carbon Nanotube Excitation‐Emission Map Simulation and Analysis

PPD Photon Counting Detector
More PPD Photon Counting Detector

Single photons detection with picosecond accuracy

More PTI FelixGX

Steady State, Lifetime and Phosphorescence Software

More SpectraLED

LED Phosphorescence Light Sources

More Ultima

Ultra Fast TCSPC Lifetime Fluorometer

Universal Fiber Coupling Solution
More Universal Fiber Coupling Solution

Connect any of our steady state and hybrid fluorometers to virtually any upright or inverted microscope!