With the increase of allergic disorders over the past years, fast and reliable techniques for characterization and identification of pollen types are required. The presented examples demonstrate that Raman microspectroscopy can be successfully applied to the analysis and the identification of airborne pollen. As it is highly chemically selective, rapid, and not requiring sample preparation, it offers an alternative to the optical microscopic methods relying only on morphological parameters. Additionnally, other spectroscopic methods (such as SPRi) provide complementary analysis for the detection of allergens. The request for such identification methods is increasing since pollinosis has become a major health issue, requiring innovative analytical characterization tools.
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