Light incident on a leaf can be absorbed by chlorophyll to commence the photosynthetic cycle. Excess energy can be liberated as heat or by emission of fluorescence and this can be used to assess the efficiency of the photosynthetic process. Because of the Kautsky effect, the amount of fluorescence emanating from photosystem II (PSII) is not constant. There is an initial rise, upon illumination followed by a decrease caused by PSII closing down, as it is unable to transfer electrons. There can also be a contribution from PSI to the overall fluorescence. Here we employ different time‐resolved fluorescence techniques to demonstrate the types of measurement that can be performed on a whole leaf.
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