A fluorometer measures the fluorescence or light emitted by different fluorescing objects. Fluorescence occurs when light of specific wavelength hits and excites electrons in a sample, and the electrons in that sample instantly emit or fluoresce light of a different wavelength. All fluorescent objects have its own fingerprint, meaning that it excites and emits predictable types of light.

Fluorescent samples produce an emission spectrum that is measured through a monochromator connected to a photomultiplier detector similar to spectrophotometer.

After exciting a sample, a Fluorometer allow us to obtain and measure various attributes of that sample’s fluorescence, including its intensity, spectral wavelengths and lifetime. The fluorescence of a sample can be an indirect probe of the sample's environment, describing the solvent-solute interactions, inter- and intramolecular interactions, and so on. Fluorescence can be used for both qualitative and quantitative analysis of samples.

Analytical measurements provided by Fluorescence Spectroscopy Instrumentation are used in multiple applications including chemistry, biochemistry, medical research, pharmaceuticals, food science, environmental studies and nanotechnology. Fluorescence spectroscopy instruments have been used to examine counterfeit banknotes, materials for optoelectronics, and proteins.

Our Fluorometers can accommodate any size work-area, and are available as compact or benchtop models, or as large modular systems, to suit your requirements. In addition, our Fluorometers can be coupled to become modular Fluorometer systems, or supercontinuum white-light laser-powered lifetime Fluorometers!

HORIBA’s Fluorometers offer incredibly fast lifetime determinations down to picoseconds, deep UV absorbance and fluorescence excitation from as short as 200 nm in the UV, all the way to near-IR wavelengths of 1000 nm.

See below for our range of Fluorometers, Components and Accessories:


EzSpec Software Intro

EzSpec Software is fast, efficient, and user friendly. The high quality graphics and intuitive design will delight students and professionals. See why EzSpec and Duetta are setting the standard for analytical instrumentation. For more info go to Duetta webpage.

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Why is Duetta different?

Listen to our interview on AZO Networks for insight into the current market of fluorometers and what makes Duetta different. 

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Duetta Wins Gold

Revolutionary Two-In-One Fluorescence and Absorbance Spectrometer Honored for Ingenuity and Innovation

HORIBA Scientific, global leader in fluorescence instrumentation and solutions, is proud to announce that its new Duetta two-in-one fluorescence and absorbance spectrometer won the Gold Pittcon Today Excellence Award in the ‘over $100 million sales’ category.

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Duetta Fluorescence and Absorbance Spectrometer

From the global leaders in fluorescence comes a new bench-top instrument and a new concept for analytical molecular spectroscopy.

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Steady-State Fluorescence Spectrofluorometers

Our steady-state fluorescence spectroscopy looks at the long-term average Fluorescence of a sample when irradiated with UV, Visible or near-IR Light.

These research-grade fluorometers are compact, benchtop or modular, and customizable for any sort of Fluorometry, be it Fluorescence detection or Phosphorescence studies your research requires.

Multifunction Steady-State and Lifetime Spectrofluorometers

HORIBA Scientific's multifunction fluorometers combine the best of both worlds: our multifunction instruments perform both steady-state and TCSPC lifetime fluorescence spectroscopy with the highest sensitivity and fast time resolution.

Lifetime Fluorescence Spectrofluorometers

Lifetime fluorescence spectroscopy or time-resolved fluorescence spectroscopy investigates the change in fluorescence over time of a sample when irradiated with UV, visible, or near-IR light. This decay in fluorescence can be measured over a wide time range: from picoseconds to milliseconds and beyond.

Fluorescence Software

HORIBA Scientific offers Fluorescence Spectroscopy software tailored precisely for the types of experiments you perform. Our software runs through the Windows® environment, and controls all aspects of your system, from control of the slits at the entrance and exit ports of your spectrograph, to detector gain, system correction files, blank-subtraction, and automated batch-processing.

In addition, our software performs post-experimental modeling using the latest in mathematical techniques, and adds the power of an advanced graphical display and flexibility to export via many popular file-formats.

Browse Products

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Fluorescence and Absorbance Spectrometer

Aqualog - Water Treatment Plant Analyzer
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Automated Organic Analysis & Early Warning Sentinel

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Modular Research Fluorometer for Lifetime and Steady State Measurements

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Steady State and Lifetime Bench-top Spectrofluorometer

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TCSPC Lifetime Fluorometer

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TCSPC Lifetime Fluorometer

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Steady State and Lifetime Nanotechnology Spectrofluorometer

Aqualog - A-TEEM Industrial QC/QA Analyzer
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A Simple, Fast, “Column Free” Molecular Fingerprinting Technology

Aqualog - Environmental Water Research Analyzer
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The Gold Standard for Water CDOM Research

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TCSPC Lifetime Kit

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Ca++ Imaging System

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Imaging camera for dynamic FLIM studies at real time video rates

Hybrid Picosecond Photon Detector (HPPD) Series
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HORIBA’s latest development in TCSPC detector technology

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Pulsed Laser and LED Light Sources

PPD Photon Counting Detector
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Single photons detection with picosecond accuracy

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Calcium Imaging Microscope

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LED Phosphorescence Light Sources

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Ultra Fast TCSPC Lifetime Fluorometer


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