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Sedimentation Analysis by Stokes’ Law with the ViewSizer™ 1000
In this application note, a 12 μm PSL standard was measured with the ViewSizer 1000 Nanoparticle Tracking Analyzer to investigate the upper limit of the instrument.
For a normal nanoparticle tracking analysis measurement, the particles are generally smaller than 1 micron and show random Brownian motion rather than settling motion. However, when particles start to get larger than 1 micron, they tend to show more settling motion than Brownian motion, requiring a different equation to size the particles. The exact size at which the particle starts to show more settling motion than Brownian motion depends on the particle’s density, but when settling is observed, the particle size can be calculated using the Stokes’ Law, which relates the speed of settling to the particle size.
The ViewSizer 1000, equipped with a high-resolution camera aimed at the side of the cuvette, normally used to size sub-micron particles through their Brownian motion, can also be used to measure the size of micron-sized particles by tracking their settling motion.
In this application note, a 12 μm PSL standard was measured with the ViewSizer 1000 Nanoparticle Tracking Analyzer to investigate the upper limit of the instrument.
Multi-Variable Pulse Nanoparticle Tracking Analysis (NTA)
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