Fluorescence

Fluorescence Spectroscopy Solutions for Environmental Measurement

Environmental sustainability remains one of the most critical challenges of our time. By engineering advanced analytical instrumentation for the environmental sciences, HORIBA actively contributes to the preservation of natural resources for future generations.

Our comprehensive line of analytical instruments addresses a diverse range of vital environmental applications, including atmospheric pollution monitoring, potable and wastewater analysis, soil contamination assessment, and the detection and characterization of microplastics.

Fluorescence spectroscopy is central to these capabilities. It is a highly sensitive, non-destructive technique that enables researchers to track organic matter dynamics, identify trace pollutants, and monitor water quality with exceptional precision and speed.

By combining unprecedented levels of sensitivity, reliability, and reproducibility with advanced data science, HORIBA analytical instruments deliver the actionable insights needed to foster a cleaner, more sustainable world.


Geology | Environment Water | Petrochemicals

Geology

Why use fluorescence in GEOLOGY? 

  • MOST MINERALS EMIT FLUORESCENCE 

 

What techniques are used to measure GEOLOGY with fluorescence? 

  • Fluorescence Spectra 
  • Fluorescence Microscopy 
  • Fluorescence & Phosphorescence Lifetimes 
  • FLIM 

 

Why use a HORIBA fluorometer in GEOLOGY? 

  • Highest guaranteed sensitivity 
  • Wide range of wavelengths 
  • Positive experience with HORIBA Raman (common in such labs) 
  • Remote fiber optics customization 

Environment Water

Why characterize WATER using fluorescence? 

  • Speed of data acquisition 
  • Specificity 
  • Sensitivity 

 

What techniques are used to measure WATER with fluorescence?\ 

  • The A-TEEM or Absorbance-Transmission fluorescence Excitation-Emission Map is key for CDOM research and monitoring. 
  • EEMs provide a tremendous amount of qualitative and quantitative spectral information not available from any other optical technique. 
  • Other techniques like UV-VIS absorbance, resistance based TOC determinations, or oxygen monitors are void of spectral selectivity and therefore the ability to ID and or quantify key organic compounds and pollutants of interest.  
  • The absorbance and emission spectra of all dissolved fluorescent compounds are resolved simultaneously. 
  • Automated chemometrics 

 

Why use a HORIBA fluorometer to measure WATER CDOM?  

  • No other commercial fluorometer comes close 
  • The fastest instruments for EEMs with the only CCD detectors. 
    • This is critical because most CDOM labs need to run hundreds of samples regularly. 
    • While others take 20-60 min per EEM, we need only 30s – 1 min  
  • True Simultaneous Absorbance-Fluorescence (patented) needed for Fluorescence Reabsorbance Correction (A-TEEM) 
    • This eliminates the need for costly and tedious dilution and  pH balancing for the hundreds of samples. 
    • So preparation time and analysis times are both diminished 
  • Only instrument to offer NIST SRM ISO standard method for CDOM EEMs complete with quantitative standard samples and instrument conditions to enable valid spectral identification. 
  • UV Excitation down to 200 nm 

Petrochemicals

Why use fluorescence to characterize PETROCHEMICALS? 

  • Petrochemicals are made of Aromatics, Dienes, Conjugated polydienes, Polyaromatics, Parafins, Cycloalkanes  
  • Many of these molecules are UV fluorescent 

 

What techniques are used to measure PETROCHEMICAL fluorescence? 

  • Synchronous Scans 
    • Fingerprint of overlapping fluorophores 
  • Fluorescence EEMs for comprehensive 3D fingerprinting 
    • EEMS: Excitation-Emission Matrices 
    • Detect multiple fluorescing components in a sample 
    • Measure many samples or same sample over different conditions 
    • Multivariate analysis used to identify components and contributions of each component (in MatLab) 
  • Fluorescence Lifetime 
    • Multiple components have different lifetimes 
    • TRES (time-resolved emission spectrum) has ability to identify spectra at different time points  

 

Why use HORIBA fluorometers to characterize PETROCHEMICALS? 

  • Highest guaranteed sensitivity (trace analysis) 
  • True Simultaneous Absorbance-Fluorescence (patented) needed for Fluorescence Reabsorbance Correction. 
  • NIST SRM ISO standard method for CDOM A-TEEMs complete with quantitative standard samples and instrument conditions to enable valid spectral identification. 
  • Optimized for petrochemical fingerprint 
  • UV Excitation down to 200 nm 
  • Experts in TCSPC 

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