One of the most frequently used rapid kinetics techniques.
Used to study fast kinetics reactions (in the millisecond timescale) between two mixed reactants.
Many applications in the chemistry and biochemistry fields.
How does it work?
Under pneumatic drive activation, the two small volumes of solutions are driven from high performance syringes through a high efficiency mixer.
The resultant mixture passes through a measurement flow cell and into a stopping syringe.
Just prior to stopping, a steady state flow is achieved.
As the solution fills the stopping syringe, the plunger hits a block, causing the flow to be stopped instantaneously.
Using appropriate techniques, the kinetics of the reaction can be measured in the cell.
Dead time < 8 ms
Observation cell optimized for best performance with SPEX® instruments
Integrates into any current SPEX® fluorescence system
Flexible thermostat format
Sample’s path constructed of chemically inert Kel-F™, silica, Teflon®, and glass
Crush-resistant flexible umbilical
Teflon® is a registered trademark of E.I. DuPont de Nemours, Inc.
700 µL (SFA-20 and SFA-20mx); 350 µL (SFA-20M and SFA-20mxM)
3 mm wide
Flow cell\'s beam height:
15 mm from base of cell to center of window
Min. vol. per determination:
100 µL per reactant
3 mm for excitation and 3 mm for emission
3 mm wide x 10 mm high for excitation and emission, specifically designed for FluoroMax® and FluoroLog® instruments
Adjustable stop volume:
Up to 20:1
5 - 80°C
Manual drive is standard; Pneumatic control optional. We recommend, however, the SFA-20mx/SPEX and SFA-20mxM/SPEX Umbilical length = 600 mm (SFA-20/SPEX and SFA-20mx/SPEX); 300 mm (SFA-20M/SPEX and SFA-20mxM/SPEX); other lengths on request
2.5 mL for SFA-20/SPEX; 1 mL for other models High-efficiency T-format mixer: Available for SFA-20/SPEX and SFA-20M/SPEX; 10 µL delay available for SFA-20mx/SPEX and SFA-20mxM/SPEX; longer delays upon request