ViewSizer 3000

멀티 레이저 나노 입자 추적 분석기

엑소좀, 바이러스, 나노입자 등 다양한 샘플의 모든 입자에 대한 분석을 위한 멀티 레이저

엑소좀, 바이러스 및 나노입자는 모두 기존의 NTA(나노입자 추적 분석) 분석기를 능가하는 넓은 크기 분포를 가지고 있습니다.
ViewSizer 3000은 동일한 샘플 내에서 다양한 크기에 걸쳐 가장 정확한 분포 및 농도 정보를 수집하기 위해 3개의 레이저를 사용하여 동시 측정이 가능합니다.
입자의 신호가 너무 밝고 한 레이저의 검출기를 포화시키는 경우 소프트웨어는 가장 정확한 크기와 농도 정보를 보장하기 위해 저출력 레이저의 데이터를 자동으로 사용합니다.
반면에 한 레이저의 산란이 너무 약하여 감지할 수 없는 경우 소프트웨어는 더 높은 출력의 레이저 데이터를 사용하여 입자를 정확하게 추적합니다.
교차 오염이 일어나지 않도록 철저한 클리닝을 진행합니다. 제거하기 쉬운 샘플 셀을 분해하여 빠르고 철저한 세척이 가능하므로 더 나은 데이터를 얻을 수 있습니다.
기존 NTA의 한계에서 벗어나 교차 오염 없는 정확한 분석이 가능합니다.

Segment: Scientific

Division: Particle Characterization

Manufacturing Company: HORIBA Instruments Incorporated

The ViewSizer 3000 uses the latest advances in nanoparticle tracking analysis (NTA) to accurately determine particle properties!

Size Measurement Range: 10 nm to 15 microns depending on sample

The ViewSizer determines particle size and size distribution details by multi-laser nanoparticle tracking analysis (NTA). Multiple lasers enable analysis of a broad range of sizes in the same sample.

Concentration Measurement Range: 5 x 10⁶ to 2 x 10⁸ particles/mL

NTA can be used to count particles in the measurement volume. A patented method corrects for the effect of particle size on effective measurement volume.

No cross-contamination

The sample cell can be completely removed and dissassembled for complete cleaning. Disassembly, cleaning, and reassembly is faster than rinsing a flow cell. Furthermore, multiple cells can be used for faster throughput or assigned to various groups in a shared (core) facility.

Featured Publications for the ViewSizer 3000:

Biological characterization using protein crystal measurements	https://bioprocessintl.com/analytical/product-characterization/biological-characterization-using-protein-crystal-measurements/
A lipase-independent pathway of lipid release and immune modulation by adipocytes	https://science.sciencemag.org/content/363/6430/989
Application of a novel new multispectral nanoparticle tracking technique	https://iopscience.iop.org/article/10.1088/1361-6501/aab940/meta
Biophysical characterization of polydisperse liposomal adjuvant formulations	https://doi.org/10.1016/j.bbrc.2020.05.156
Characterisation of particles in solution – a perspective on light scattering and comparative technologies	https://doi.org/10.1080/14686996.2018.1517587
Cyclodextrin Reduces Intravenous Toxicity of a Model Compound	https://doi.org/10.1016/j.xphs.2019.01.004
Development and anti-Candida evaluation of the vaginal delivery system of ... nanosuspension-loaded thermogel	https://doi.org/10.1080/1061186X.2018.1434660
Electrochemical sensor based on F,N-doped carbon dots decorated laccase for detection of catechol	https://doi.org/10.1016/j.jelechem.2019.03.071
Light scattering by pure water and seawater: the depolarization ratio and its variation with salinity	https://doi.org/10.1364/AO.58.000991
Lipid Nanoparticle-Delivered Chemically Modified mRNA Restores Chloride Secretion in Cystic Fibrosis	https://doi.org/10.1016/j.ymthe.2018.05.014
Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells	https://doi.org/10.1038/s41598-020-67039-w
Multifunctional Nanocomposites Based on Liposomes and Layered Double Hydroxides Conjugated with Glycylsarcosine for Efficient Topical Drug Delivery to the Posterior Segment of the Eye	https://doi.org/10.1021/acs.molpharmaceut.8b01136
Particle size analysis of polydisperse liposome formulations with a novel multispectral advanced nanoparticle tracking technology	https://doi.org/10.1016/j.ijpharm.2019.06.013
Review of nanoparticles in ultrapure water: definitions and current metrologies for detection and control	https://www.ovivowater.com/review-of-nanoparticles-in-ultrapure-water-definitions-and-current-metrologies-for-detection-and-control/
Spark erosion as a high-throughput method for producing bimodal nanostructured 316L stainless steel powder	https://doi.org/10.1016/j.powtec.2018.01.012
Synthesis and Characterization of EGFR-Targeted Immunoporphysomes	http://hdl.handle.net/1807/89548
Synthesis of Ultrasmall Synthetic Melanin Nanoparticles by UV Irradiation in Acidic and Neutral Conditions	https://pubs.acs.org/doi/abs/10.1021/acsabm.9b00747
Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles \| Protocol (jove.com)	https://doi.org/10.3791/62447
Isolation and characterization of EV in Saliva of Children with Asthma	https://evcna.com/article/view/3962
Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential	https://doi.org/10.1016/j.bbi.2020.12.007
Skeletal muscle tissue secretes more extracellular vesicles than white adipose tissue and myofibers are a major source ex vivo but not in vivo	https://doi.org/10.1101/2020.09.27.313932
Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands	https://www.nature.com/articles/s41598-021-84809-2
Purification of Cas9 - RNA Complexes by Ultrafiltration	https://doi.org/10.1002/btpr.3104
Assessment of Surface Glycan Diversity on Extracellular Vesicles by Lectin Microarray and Glycoengineering Strategies for Drug Delivery Applications	https://doi.org/10.1002/smtd.202100785
Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration	https://doi.org/10.1172/jci.insight.155928
Circulating Extracellular Vesicles From Septic Mice Induce Brain Inflammation via Mirna Cargo	http://hdl.handle.net/10713/19055

Measurement Range	10 nm to 15 μm
Typical Sample Volume	350 µL to 1.25 mL
Typical Sample Concentration (Sample dependent)	5 x 10⁶ to 2x10⁸ particles/mL
Sample Temperature Range (Controlled)	10° C to 50° C +/- 0.1° C
Dimensions	55 cm W x 66 cm D x 35 cm H
Weight	27 kg
Operational Environment	15° C to 30° C with < 85% RH

Particle Analysis in Vaccine Manufacturing and Development

Size matters in vaccine delivery systems. Nanoparticles smaller than 200 nm generally present a greater immunogenic response than micro-particles larger than 1 micron.

Achieving Infectious Titer with Multi-laser Nanoparticle Tracking Analysis (NTA)

In this note, the upstream process of analyzing viral preparations is addressed, as well as the use of multi-laser nanoparticle tracking analysis (NTA) as a cost and time efficient method to measure size, count virus particles, and correlate to infectious titer.

Optimization of an Emulsion Polymerization Process and Product Through Nanoparticle Concentration Analysis

This note covers the history, theory and processes used for emulsion polymerization. It also examines the importance of measuring the concentration of latex nanoparticles produced by emulsion polymerization for a biotech application developed by the Gianneschi lab at UCSD.

Predicting Whiskey Shelf Stability with Particle Size Distribution

Careful control of the particulates in whiskey is an important step to quality flavor and color. Inadequate monitoring of particle size impedes the final product stability, consistency, quality, and price. In this note, the particle science behind these determining factors will be thoroughly examined and explained.

Optimization of High-Performance Nanostructured Powder Metallurgy Materials

In this note, we will briefly explore the history of powder metallurgy and then examine the importance of powder quality to the production of nanomaterials.

Nanobubble Size and Concentration Analysis with Multi-laser NTA

This note analyzes carbon dioxide and oxygen gas nanobubbles used for pain management to demonstrate multi-laser Nanoparticle Tracking Analysis’s ability to characterize a mixture of nano and microbubbles without sample manipulation.

Protein aggregation is an important concern when developing and manufacturing biotherapeutics. It is important to quantify protein aggregation and particle formulation as this data is needed to guide formulation development, evaluate handling requirements, and monitor product quality.

Size Distribution and Concentration of Extracellular Vesicles

In this study, we demonstrate the ViewSizer 3000’s capabilities as a next generation NTA based analysis instrument in order to accurately and efficiently measure and characterize extracellular vesicles (EV) via particle size and concentration.

Characterization of Sub-Visible Particles in Protein Therapeutic Formulations

The ViewSizer™ 3000 enables scientists to visualize and quantify the kinetics of protein aggregation in real-time for biologic therapeutics under a variety of stress conditions.

Nanotechnology is extremely diverse, ranging from novel extensions of conventional device physics, to completely new approaches based upon molecular self-assembly and to developing new materials with dimensions on the nanoscale. Materials reduced to the nanoscale can show different properties compared to what they exhibit on a macroscale, enabling unique applications.

Virtually any metal can be made into a powder through processes including atomization, electrolysis, chemical reduction, and size comminution. The metal powder can then be used to fabricate parts from simple to complex shape. The technique and study of these processes is known as powder metallurgy, a field of study well acquainted with particle characterization. The size distribution and shape of the powder metal particles influences the physical properties of the parts created.

Particle Analysis in Polymers & Plastics

Polymers are large molecules made from repeating structural units connected by covalent chemical bonds. We often use the word ‘plastic’ to refer to polymeric materials, but polymers also include a wide range of natural materials such as proteins, DNA, cellulose, shellac and amber.

Quantifying Trace Amount of Nanoparticles in Drinking Water

The concern of water contamination from nanoplastics (≤ 1 µm) is a study receiving close scrutiny from FDA and EPA alike. Nanoparticles can gain access through inhalation, ingestion, or dermal exposure and have greater cellular uptake than those bigger in size. Nanoparticles subsequently pose a greater impact on health.

A virus particle consists of several parts including the genetic material (DNA or RNA), a protein coat that protects these genes, and in some cases an envelope of lipids that surrounds the protein coat when they are outside a cell.

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ViewSizer 3000 Brochure

Size: 2.5 MB

Particle Overview Brochure

Size: 2.32 MB

Particle Guidebook