A-TEEM (Absorbance, Transmittance, and Excitation-Emission-Matrix) spectroscopy, combined with multivariate analysis, is a rapid, non-invasive, and cost-effective technique for comprehensively characterizing cell culture media, providing molecular fingerprints to monitor lot-to-lot variability and detect subtle compositional changes that directly impact cell growth and final product quality.
BioPharm International recently published an article on this topic, titled 'A-TEEM: A Novel, Powerful Tool for Evaluating and Monitoring Cell Culture Media'. The publication highlights how A-TEEM, by enabling the simultaneous optical characterization of media, offers molecular-level insights into composition, thereby enhancing quality control and ensuring media consistency in biopharmaceutical manufacturing. Specifically, the article demonstrates the effectiveness of A-TEEM in assessing lot-to-lot variability and providing a robust, cost-effective approach for ensuring media quality before its use in bioreactors.
Read the full story on BioPharm International to explore the data and case studies.
A: A-TEEM (Absorbance, Transmittance, and Excitation-Emission-Matrix) provides a unique molecular fingerprint of the media, allowing for sensitive identification of a wide range of fluorophores and chromophores. This is critical for raw material QC screening and allows companies to effectively assess the quality of basal and feed media before the addition of cells. This early characterization detects subtle compositional changes that could otherwise impact cell growth and product yield.
A: A-TEEM is applied to monitor process consistency by detecting and tracking lot-to-lot variability in media composition, even in commercially available GMP-grade cell culture media. Combined with multivariate analysis (like PARAFAC or PCA), A-TEEM allows biopharma teams to monitor compositional changes over time. This provides predictive capabilities for the early detection of potential media degradation and helps ensure media consistency throughout the manufacturing process, reducing unexpected cell culture performance issues.
A: A-TEEM offers significant efficiency and cost advantages due to its rapid, non-invasive nature. Data acquisition takes mere seconds, not minutes. It has a low per-measurement cost and is considered a sustainable/green method because it eliminates the need for solvents, columns, standards, or waste disposal required by chromatographic techniques. This makes it suitable for real-time Process Analytical Technology (PAT) deployment in the manufacturing setting.
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