Sugar content of beverages final products like juices, sodas or ice teas is particularly controlled as there is a globally important consumption of sugary drinks that is not without any consequence on the health of world population. Overconsumption of sugary drinks is related to abdominal obesity and to a risk increase to develop type 2 diabetes. Content determination of sugar, as well as of its substitutes, so called sweeteners such as the “chemical” aspartame or the “natural” stevia, is of interest in final products as well as during different stages of beverages and food products production chains. In this application note, we show how Raman spectroscopy is used to identify and determine sugar/sweetener concentrations of solutions using MVAPlus multivariate analysis application. Moreover, we demonstrate how this is performed in an automated routine analysis combined to the MultiWell application.