Bioengineering

Bioengineering, the study of molecular biology and engineering concepts, using biology with physics, chemistry and mathematics to solve real-world problems for life science researchers. Fields include biomedical engineering, biomechanics, biomaterials, and clinical engineering. Horiba Scientific is a leading supplier of instruments to these research fields.

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Production of a single domain antibody Q17c directed against recombinant HER2 protein and its binding study by Surface Plasmon Resonance imaging technology
Production of a single domain antibody Q17c directed against recombinant HER2 protein and its binding study by Surface Plasmon Resonance imaging technology
This application note shows how binding kinetics of single-domain antibodies can be studied with SPRi technology. QVQ produces single-domain antibodies from camelids (sdAbs or VHH). Like conventional Abs, sdAbs are able to bind specific epitopes with high binding affinity. The small size of sdAbs allows for enhanced tumor penetration and fast blood clearance, features that are favorable for in vivo imaging applications. Here, the binding of the anti-HER2 sdAb Q17c to recombinant HER2 protein was assessed using Surface Plasmon Resonance imaging (SPRi). An optimization of immobilization conditions was performed for Q17c and its specific antigen HER2 using a single SPRi-Biochip.
Visualizing local viscosity using fluorescence lifetime microscopy
DynaMyc with FLIM (fluorescence lifetime imaging)
The use of fluorescent molecules, known as molecular rotors, is advantageous in estimating the local (nanoscale) viscosity in microheterogeneous systems, since it just requires the measurement of their fluorescence lifetime.

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