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Cell Culture Media Analysis: Qualitative and Quantitative Analysis using the Veloci™ BioPharma Analyzer

Cell culture media used in bioprocessing are inherently complex, making it difficult to quickly assess lot-to-lot variability, track key components such as amino acids and vitamins, and manage time-consuming sample preparation and separations for analysis. This high matrix complexity presents major challenges for monitoring and ensuring consistency, while optimizing performance.

Traditional measurements, such as pH and osmolality, offer only limited insight, whereas more comprehensive techniques, like HPLC or LC-MS, though powerful, are often slow, and require extensive sample preparation. To overcome these limitations, A-TEEM™ (Absorbance-Transmittance Excitation Emission Matrix) spectroscopy provides a powerful alternative—enabling rapid, detailed molecular fingerprinting of complex media without the need for separations. This makes it ideally suited for efficient, high-resolution analysis of media composition in both research and manufacturing environments.

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Cell Culture Media Analysis: Qualitative and Quantitative Analysis using the Veloci™ BioPharma Analyzer
This study demonstrates the potential of A-TEEM™ spectroscopy as a rapid and reliable tool for evaluating lot-to-lot variability in complex cell culture media. By leveraging the distinct spectral fingerprints of aromatic amino acids and vitamins, A-TEEM enables sensitive tracking of compositional differences without the need for extensive sample preparation or lengthy separations.
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A-TEEM Spectroscopy

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