Microfluidic Devices for the Rapid Analysis of Individual Mammalian Cells

By: Christopher T. Culbertson

28 February 2011

We are developing techniques to culture and analyze single non-adherent cells on microfluidic devices. The microfluidic modules will be capable of culturing up to 20,000 cells over a period of several days. The cells are arranged in such a way that one of the daughter cells after each division will be released for downstream analysis. The released daughter cells are transported through a channel manifold where they can be subjected to external stimuli in order to examine how they respond. The cells can then be incubated and transported to an area of the chip where they will be lysed. The lysate is then separated and the components of interest detected and quantified.

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