Training course

Training

SPRi - OpenPlex

Overview

  • Reference: SPR1
  • Dates:
    • February 11-13, 2019
    • June 17-19, 2019
  • Who should attend: OpenPlex users or people wishing to acquire an OpenPlex
  • Duration: 3 days (From 9 am to 5 pm)

Objectives

  • Learn how to optimize the experimental conditions according to the interaction to be analyzed
  • Learn how to make biochip spotting
  • Master the use of OpenPlex
  • Learn how to analyze a kinetic data

Program

Day 1

  • Introduction to kinetics of interaction: What is a «good» kinetic?
  • Which biochip to choose according to the molecules to immobilize?
  • How to optimize spotting (concentrations, buffers, reference, ...)?
  • The important experimental parameters to be tested for SPRi (pH, stroke buffer, temperature, ...)
  • Advice to be applied during the realization of the experiment (duration of kinetics, flow rate, regeneration, ...)
  • Case studies

 

Day 2

  • Choose the right surface chemistry as a function of immobilized ligands
  • Optimize experimental immobilization conditions (spotting buffer, pH, ligand concentration, duration of needle rinses / dryings, etc.)
  • Master the different steps to program a spotting matrix*
  • Perform spottings

 

Day 3

  • Optimize the SPRi experimental conditionsaccording to the interaction to be analyzed
  • Master the use of OpenPlex
  • Two models of interactions will be studied:
    - Study of a first interaction (protein / protein)
    - Study of a second interaction (DNA / DNA)
  • Analysis of the data, and description of the different analysis software.

                                                                           

 *The choice of the spotter used must be defined when registering

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