Training course


SPRi - OpenPlex

These trainings are held in France or at your location and are conducted in French or English.


  • Reference: SPR1
  • Dates:
    • February 10-12, 2020
    • June 15-17, 2020
  • Who should attend: OpenPlex users or people wishing to acquire an OpenPlex
  • Duration: 3 days (From 9 am to 5 pm)


  • Learn how to optimize the experimental conditions according to the interaction to be analyzed
  • Learn how to make biochip spotting
  • Master the use of OpenPlex
  • Learn how to analyze a kinetic data


Day 1

  • Introduction to kinetics of interaction: What is a «good» kinetic?
  • Which biochip to choose according to the molecules to immobilize?
  • How to optimize spotting (concentrations, buffers, reference, ...)?
  • The important experimental parameters to be tested for SPRi (pH, stroke buffer, temperature, ...)
  • Advice to be applied during the realization of the experiment (duration of kinetics, flow rate, regeneration, ...)
  • Case studies


Day 2

  • Choose the right surface chemistry as a function of immobilized ligands
  • Optimize experimental immobilization conditions (spotting buffer, pH, ligand concentration, duration of needle rinses / dryings, etc.)
  • Master the different steps to program a spotting matrix*
  • Perform spottings


Day 3

  • Optimize the SPRi experimental conditions according to the interaction to be analyzed
  • Master the use of OpenPlex
  • Two models of interactions will be studied:
    - Study of a first interaction (protein / protein)
    - Study of a second interaction (DNA / DNA)
  • Analysis of the data, and description of the different analysis software.


 *The choice of the spotter used must be defined when registering

Our trainers are experts in each technique. They will provide trainings advice and guidance to make the most of your HORIBA Scientific instrument. You will gain confidence and experience in the analysis of your samples.

Certificates are given to every attendee for every course.


Do you have any questions or requests? Use this form to contact our specialists.