Training course

Training

SPRi - XelPleX

Overview

  • Reference: SPRi2
  • Dates:
    • May 14-17, 2018
    • October 22-25, 2018
  • Who should attend: XelPLeX users or people wishing to acquire an XelPleX
  • Duration: 3 days (From 9 am to 5 pm)

Objectives

  • Learn how to optimize the experimental conditions according to the interaction to be analyzed
  • Learn how to make a biochip spotting
  • Master the use of XelPleX
  • Learn how to analyze a kinetic data

Program

Day 1

  • Introduction to kinetics of interaction: What is a «good» kinetic?
  • Which biochip to choose according to the
    molecules to immobilize?
  • How to optimize spotting (concentrations, buffers,
    reference, ...)?
  • The important experimental parameters to be
    tested in SPRi (pH, running buffer, temperature,...)
  • Advice to be applied during the realization of
    the experiment (duration of kinetics, flow rate,
    regeneration, ...)
  • Case studies

 

Day 2

  • Choose the right surface chemistry as a function
    of immobilized ligands
  • Optimize experimental immobilization conditions
    (spotting buffer, pH, ligand concentration, duration
    of needle rinses / dryings, etc.)
  • *Master the use of the spotter (master the different
    steps to program a spotting matrix)
  • Performing spottings

 

Day 3

  • Optimize the SPRi experimental conditionsaccording to the interaction to be analyzed
  • Master the use of OpenPlex
  • Two models of interactions will be studied:
    - Study of a first interaction (protein / protein)
    - Study of a second interaction (DNA / DNA)
  • Analysis of the data, and description of the
    different analysis software.

 

Day 4

  • Reminder on interaction kinetics
  • Use of EzFit software. Analysis of different
    interactions (Ac / Ac, DNA / DNA, Protein / small
    molecule, ...)
  • Carrying out analysis in «classical» mode (injection
    of several concentrations of the analyte) and
    in «single-injection» mode (injection of a single
    concentration of the analyte)

                                                                             

     *The choice of the spotter used must be defined when registering