Training course

Training

SPRi - XelPleX

These trainings are held in France or at your location and are conducted in French or English.

Overview

  • Reference: SPRi2
  • Dates:
    • May 11-14, 2020
    • October 19-22, 2020
  • Who should attend: XelPleX users
  • Duration: 4 days (From 9 am to 5 pm)

Objectives

  • Learn how to optimize the experimental conditions according to the interaction to be analyzed
  • Learn how to make a biochip spotting
  • Master the use of XelPleX
  • Learn how to analyze a kinetic data

Program

Day 1

  • Introduction to kinetics of interaction: What is a «good» kinetic?
  • Which biochip to choose according to the molecules to immobilize?
  • How to optimize spotting (concentrations, buffers, reference, ...)?
  • The important experimental parameters to be tested in SPRi (pH, running buffer, temperature,...)
  • Advice to be applied during the realization of the experiment (duration of kinetics, flow rate, regeneration, ...)
  • Case studies

 

Day 2

  • Choose the right surface chemistry as a function of immobilized ligands
  • Optimize experimental immobilization conditions (spotting buffer, pH, ligand concentration, duration of needle rinses / dryings, etc.)
  • *Master the use of the spotter (master the different steps to program a spotting matrix)
  • Performing spottings

 

Day 3

  • Optimize the SPRi experimental conditions according to the interaction to be analyzed
  • Master the use of XelPleX
  • Two models of interactions will be studied:
    - Study of a first interaction (protein / protein)
    - Study of a second interaction (DNA / DNA)
  • Analysis of the data, and description of the different analysis software.

 

Day 4

  • Reminder on interaction kinetics
  • Use of EzFit software. Analysis of different interactions (Ac / Ac, DNA / DNA, Protein / small molecule, ...)
  • Carrying out analysis in «classical» mode (injection of several concentrations of the analyte) and in «single-injection» mode (injection of a single concentration of the analyte)

                                                                             

     *The choice of the spotter used must be defined when registering

Our trainers are experts in each technique. They will provide trainings advice and guidance to make the most of your HORIBA Scientific instrument. You will gain confidence and experience in the analysis of your samples.

Certificates are given to every attendee for every course.

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