LabRAM HR Evolution

LabRAM HR Evolution

Confocal Raman Microscope

The LabRAM HR Evolution Raman microscopes are ideally suited for both micro and macro measurements, and offer advanced confocal imaging capabilities in 2D and 3D. The true confocal Raman microscope enables the most detailed images and analyses to be obtained with speed and confidence. With guaranteed high performance and intuitive simplicity, the LabRAM HR Evolution is the ultimate instrument for Raman spectroscopy.  They are widely used for standard Raman analysis, PhotoLuminescence (PL), Tip Enhanced Raman Spectroscopy (TERS) and other hybrid methods.

Move from microscopy to the nanoscopy world with an easy AFM upgrade

Segment: Scientific
Manufacturing Company: HORIBA France SAS

High spatial and spectral resolutions
HORIBA Scientific’s optimized design results in the most sensitive instruments on the market, with high throughput diffraction limited spatial resolution. Spectrally, the high resolution spectrometer allows subtle sample information such as crystallinity, polymorphism, strain and other band analysis to be characterized with ease.

Cover your sample from UV to NIR
Compatibility with a wide range of laser wavelengths and the possibility of mounting up to three detectors enables the measurement wavelength range to be extended from 200nm to 2200nm. The optimized UV configuration offers the best solution for UV Raman analysis with wavelengths below 400nm. Such performance opens up other spectroscopic techniques such as UV Raman, resonance Raman and photoluminescence, allowing detailed sample characterization from many varied materials.

Ease of use and ergonomy
The fully automated intelligent design of the LabRAM HR Evolution ensures ultimate performance coupled with ease of use; and the LabSpec 6 software allows full benefit from the unrivalled performances of the LabRAM HR Evolution at the touch of a button.

Ultra-fast confocal imaging
The LabRAM HR Evolution includes the unique SWIFT™ and DuoScan™ fast Raman imaging technologies.
DuoScan™ is a confocal imaging mode, with high precision, ultra-fast rastering mirrors creating variable sized laser macro-spots, and also allowing nano-step mapping from deep UV to NIR.
SWIFT™ offers confocal Raman mapping with CCD integration times down to 1 ms per point and below.

The unique combination of innovative optics, detectors and software combine to provide true confocal Raman imaging with an unmatched speed of data acquisition.

Raman-AFM and TERS compatible
The integral flexibility of the LabRAM HR Evolution means that it is the ideal platform for combined Raman-AFM analysis, and research to TERS (tip enhanced Raman scattering) nano-Raman

Ultra-Low Frequency Module
The LabRAM HR Evolution combines simple access to very low frequencies down to 5cm-1 with a high throughput single stage spectrometer, using the ULF Module. The new generation of notch and bandpass filters allows additional sample characterization to be made in a spectral region rarely available with other basic spectrometers.

Automated particle location and chemical ID
ParticleFinder offers a user friendly tool for automated location, characterization and Raman analysis of particles. Hundreds or thousands of particles can be quickly located and characterized using the LabRAM HR Evolution’s advanced Raman capabilities.

Find the best conditions for the analysis of your sample
The sampling flexibility of the LabRAM HR Evolution ensures that your research can be fully optimized. Standardly equipped with the upright microscope, an open-space microscope can be installed giving you free space under the objective to adapt numerous accessories like large cryostats, broad travel range stages…  For life science research, the inverted microscope offers ideal sampling for cells and tissues.

CaptuR laser trapping
A unique and integrated laser trapping solution ideal for fine particle control during microscopic analysis.

Furthermore, the Transmission Raman accessory can be easily installed on the LabRAM HR Evolution for bulk analysis of opaque/turbid materials; and the SuperHead probes enable in-situ monitoring of reactions or remote analysis.

 

Co-localized microscopy techniques for pyrite mineral spatial characterization
Co-localized microscopy techniques for pyrite mineral spatial characterization
In this study we have chosen to investigate pyrite and its surrounding minerals in order to identify the different mineral phases as well as the chemical variations from micro- to nano-scale. Using the different microscopes instruments and being co-localized allows a comprehensive characterization of the sample and a precise superimposition of all the images.
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Proton Exchange Membrane Fuel Cell Bipolar Plate Analyses by GD-OES and Raman
Proton Exchange Membrane Fuel Cell Bipolar Plate Analyses by GD-OES and Raman
Bipolar plates are key components of proton exchange membrane fuel cells – they notably distribute fuel gas and air and conduct electricity. Various materials and surface treatments have been developed to improve their properties. Here, we described a reverse engineering study on a bipolar plate from a commercial vehicle using GD-OES and Raman spectroscopy. The analyses revealed that the plate had an amorphous carbon coating on a titanium base plate.
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Three Dimensional Raman Imaging
Three Dimensional Raman Imaging
Segmented channel waveguides have been fabricated in single crystal KTiOPO4 through a topotactic process of partial cation exchange. The ion-exchanged waveguides maintain the high nonlinear susceptibility of KTiOPO4 to function as frequency doubling laser light sources. We apply three dimensional (3D) Raman imaging to understand and characterize the changes to the chemical bonding and crystalline structure as well as measure the volumetric structure of the waveguide segments.
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Raman Microscopy Applied to Polymer Characterization: An Overview
Raman Microscopy Applied to Polymer Characterization: An Overview
Raman microscopy is an excellent tool to address the polymer research. Raman microscopy can be used to characterize raw materials, to inline or outline monitor polymerization process, to investigate orientation and crystallization changes, and also to control the quality and traceability of genuine products, by understanding defects and compounds distribution. In this article we present how HORIBA Raman microscopy solutions can support the polymer chemical and structural understanding.
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Raman Spectroscopy Applied to the Lithium-ion Battery Analysis
Raman Spectroscopy Applied to the Lithium-ion Battery Analysis
The application note explains how the Raman Spectroscopy can be helpful in the analysis of cathodes and anodes in Li-ion batteries. Today’s state of art of technology requires more reliable, more efficient and powerful energy sources. Lithium-ion batteries are thus of high interest. Raman spectroscopy adapts to the different stages of life of these batteries, such as the characterization of new materials for more flexible systems, failure analysis; but also more standard analysis of used material during charge/discharge process, including structural and electronic properties, and even robust, automated QC tests.
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Insights into thrombosis mechanisms using high resolution SERS
Insights into thrombosis mechanisms using high resolution SERS
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Direct identification of clinically relevant microorganisms
Direct identification of clinically relevant microorganisms
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Raman Analysis of Single Bacteria Cells
Raman Analysis of Single Bacteria Cells
Traditionally, Raman has been a technique of the material scientist, physicist or chemist, but as instrumentation continues to evolve, the power of Raman in biological and medical applications is fast being realized, not least because of the high information content provided and an excellent tolerance for water.
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Raman Investigation of Micro-organisms on a single cell level
Raman Investigation of Micro-organisms on a single cell level
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Raman Imaging of monkey brain tissue
Raman Imaging of monkey brain tissue
Fast and non-invasive methods for clinical and non clinical investigations for biological tissue are more and more required. Raman imaging at micro scale can answer to crucial questions about the monkey brain tissue morphology and structural evolution.
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Raman Analysis of Sperm Nuclear DNA Integrity
Raman Analysis of Sperm Nuclear DNA Integrity
Raman Spectroscopy was evaluated as a non-invasive method of analysis of sperm DNA and the influence of UV irradiation on the sperm. The results show that Raman Spectroscopy, combined with multivariate analysis provide the reproducible and accurate information on DNA of sperm and the effect and location of damage.
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SERS Analysis of single living lymphocytes
SERS Analysis of single living lymphocytes
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Char of Compounds in a pharmaceutical drug product
Char of Compounds in a pharmaceutical drug product
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Characterization and mapping of active pharmaceutical ingredients and excipients in a tablet using Raman and IR spectroscopy
Characterization and mapping of active pharmaceutical ingredients and excipients in a tablet using Raman and IR spectroscopy
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Polymorphy in pharmaceuticals by Raman Spectroscopy
Polymorphy in pharmaceuticals by Raman Spectroscopy
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Raman Analysis and characterization of pharmaceuticals
Raman Analysis and characterization of pharmaceuticals
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Raman Microscopy in Pharmaceutical Salt Analysis
Raman Microscopy in Pharmaceutical Salt Analysis
Pharmaceutical and crystallographic samples typically require detailed characterization and analysis to optimize a samples stability, physical properties and indeed general efficacy where an active drug substance is involved.
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Investigating the atherosclerosis process by monitoring lipid deposits including cholesterol and free fatty acids
Investigating the atherosclerosis process by monitoring lipid deposits including cholesterol and free fatty acids
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Pharmaceuticals under humidity controlled atmosphere
Pharmaceuticals under humidity controlled atmosphere
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Soap compound investigation by Raman mapping
Soap compound investigation by Raman mapping
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In Vivo Raman measurements of Human Skin
In Vivo Raman measurements of Human Skin
Confocal Raman spectroscopy is beginning to be recognized as a high potential technique for the non invasive study of biological tissues and human skin under in vivo conditions. Raman spectroscopy can be applied to obtain information regarding the molecular composition of the skin down to several hundred micrometers below the skin surface.
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Graphene Studies using Raman Spectroscopy
Graphene Studies using Raman Spectroscopy
Graphene is a new nanomaterial which may partially replace silicon in microcircuits and computer chips in the future. In order to better understand its quality characteristics, fast reliable techniques that deliver the right property measures are needed. Raman spectroscopy has emerged as a key technique for studying this exceptional material.
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SWNT Quality Control by Raman Spectroscopy
SWNT Quality Control by Raman Spectroscopy
Raman has shown a high potential in characterising the SWCNTs' structure. The correlation between knowledge about structure with physical and chemical properties about the tubes make the technique extremely powerful to control the quality of the SWCNTs for specific applications. Raman spectrometer capabilities like spatial resolution, spectral resolution and excitation wavelength versatility have been examined. Beside Raman, preliminary fluorescence studies are describing the potential of the technique.
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Coloured Diamond Defect Idenditication by Raman Diffusion and Photoluminescence
Coloured Diamond Defect Idenditication by Raman Diffusion and Photoluminescence
The colour enhancement treatment on native brown and yellow diamonds can be highlighted by Photo Luminescence analyses performed with the Raman spectrometer LabRAM HR. The PL signature of green and violet diamonds has also been recorded. The defect centres responsible of the colour of the diamonds have all been detected and assigned. This proves the Raman spectrometer to be a very good tool to investigate the fine defects in the Diamond structure by Photoluminescence analysis.
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Derivation of Physical Parameters from Raman Spectra of Hard Carbon Films
Derivation of Physical Parameters from Raman Spectra of Hard Carbon Films
The Raman spectra of elemental carbon materials are known to be sensitive to polymorphy. For hard carbon films, the spectra of amorphous and diamond-like carbons can be band-fit to separate the contributions of the "graphitic carbon" (G band) from the "disordered carbon" (D band). The spectral behaviour of carbon films has been empirically correlated with thin film physical properties such as hardness, durability, optical transparency, electrical conductivity, thermal conductivity and corrosion resistance, and can be of use for prediction of these properties without extensive alternative testing. The DiskRam has been designed to automate the collection of Raman spectra from hard carbon coatings on computer hard disk media and the extraction of parameters that are well correlated with the properties of the films. The extracted information is output in spreadsheet format for SPC at a manufacturing facility.
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Impact of Raman Spectroscopy on Technologically Important Forms of Elemental Carbon
Impact of Raman Spectroscopy on Technologically Important Forms of Elemental Carbon
The Raman spectra of the various forms of elemental carbon are very sensitive to the type of nearest neighbour bonding, and to intermediate and long range order. In many cases Raman spectroscopy is the technique of choice for characterization of carbon materials. Correlation of Raman spectral features with tribological properties can facilitate the deposition of carbon films.
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Number of Layers of MoS2 Determined Using Raman Spectroscopy
Number of Layers of MoS2 Determined Using Raman Spectroscopy
The two methods - Analysis of fingerprint modes (intralayer) and Analysis of low-frequency modes (interlayer) - give complementary results and allow the determination of the number of MoS2 layers. Method 2 (using low frequency modes) gives excellent contrast; however it does not show single layer regions (which is related to the nature of the modes, rising from interaction between at least two layers). Method 1 (using fingerprint modes) shows all the layers, but the contrast is poorer, particularly for higher numbers of layers. The best result can be obtained combining the two methods. All the measurements (low-frequency and fingerprint) were done using ultra-low frequency ULFTM filters which allow a high throughput measurement in a full Raman range, down to <10 cm-1.
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Characterization of MoS2 Flakes using TEOS
Characterization of MoS2 Flakes using TEOS
Both TEPL and TERS images are well correlated with AFM morphological images obtained simultaneously, and all are consistent in revealing the nature (number of layers) of MoS2 flakes. Upon deconvolution, the TEPL signal is even capable of revealing local inhomogeneities within a MoS2 flake of 100 nm size. Kelvin probe measurement supports TEPL and TERS measurements and adds to the power of such tip-enhanced combinative tools. TEOS characterization of 2D materials is likely to contribute to further deployment of these materials into commercial products through a better understanding of their electrical and chemical properties at the nanoscale.
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Combined Raman and Photoluminescence Imaging of 2D WS2
Combined Raman and Photoluminescence Imaging of 2D WS2
Raman and photoluminescence spectroscopy reveal different aspects of the solid state structure of 2D materials. Raman and photoluminescence imaging performed simultaneously with one instrument reveals the spatial variation of the solid state structure and electronic properties of 2D crystals that is not revealed in reflected white light imaging. That ability should allow materials scientists to better design and fabricate electronic and optoelectronic devices based upon 2D crystals.
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Microscopic Measurement of Diffusion
Microscopic Measurement of Diffusion
In order to engineer materials with controlled diffusive properties, we need to be able to measure the diffusive process in situ. Ideally, we would like a technique that provides molecular information with resolution on a microscopic scale, that is non destructive of the samples, and can be set up and used on a laboratory bench top with a minimum of time and effort involved in sample preparation. Raman microprobe spectroscopy is an ideal candidate for this type of studies. Use of visible light combined with a confocal microscope provides a probe with spatial resolution of a micron or better, and coupling of such a microscope to a modern Raman spectrometer equipped with holographic notch filters and CCD multi channel detectors allows rapid acquisition of Raman spectra which can be correlated with the chemical state of the species and its physical environment.
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Observing Oxidating Kinetics on an aluminium alloy surface with Fluorescence mapping
Observing Oxidating Kinetics on an aluminium alloy surface with Fluorescence mapping
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Archaeometric analysis of ancient pottery in a church
Archaeometric analysis of ancient pottery in a church
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Archaeometric analysis of ancient pottery
Archaeometric analysis of ancient pottery
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The non destructive and in-situ analysis of pigments
The non destructive and in-situ analysis of pigments
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Strain Measurements of a Si Cap Layer Deposited on a SiGe Substrate, Determination of Ge Content
Strain Measurements of a Si Cap Layer Deposited on a SiGe Substrate, Determination of Ge Content
Raman spectroscopy is a very well suited technique to determine both Ge fraction and strain in SiGe layers and Si cap layers. Moreover the possibility of using both UV and visible excitation lines on the same instrument is essential to study structures made up of a Silicon cap layer on top of a SiGe layer. The relative Ge content in the constant Si1-xGex layer is calculated from the visible Raman spectrum and the strain of the cap Si layer is derived from the UV Raman spectrum.
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Concentration Profile Measurements in Polymeric Coatings During Drying by Means of Inverse-Micro-Raman-Spectroscopy
Concentration Profile Measurements in Polymeric Coatings During Drying by Means of Inverse-Micro-Raman-Spectroscopy
The coupling of the power of confocal Raman microscopy to the inverted sampling geometry has enabled detailed investigations to be made of solvent and water based coating systems, providing important information on the processes and chemistry that occurs at the coating interface and within.
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Localisation of Polymeric Phases by Raman Microscopy Mapping Components of a Blend in a Plane and Depth Profiles of Laminated Film
Localisation of Polymeric Phases by Raman Microscopy Mapping Components of a Blend in a Plane and Depth Profiles of Laminated Film
Blending, an alternative method for engineering products that combines the properties of polymer types is a physical mixing. It has the advantage of being not only simple and inexpensive, but also allows for re-cycling used material. Incompatibility or non-miscibility of the differing chemical components is often an issue in the final performance of the polymer product. The first part of this note concerns the dispersion of the two components in a polyethylene-polybutylene terephthalate blend. The chemical imaging capabilities of the LabRAM are used to get this information. The second part deals with the depth analysis of laminated films made of different polymer layers.
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Transmission Raman Spectroscopy: Review of Applications
Transmission Raman Spectroscopy: Review of Applications
The transmission design has demonstrated to be the technique of choice whenever Raman spectral information of a bulk material is required. It has already proven its utility for pharmaceutical applications, as tablets or even powder mixtures are good candidates for this measurement mode. However, transmission Raman might be applied successfully to other sample types, such as polymers, bio-tissues or any translucent material, and can be envisaged for evaluating the content of product inside a package. In addition, as TRS provides a global spectral information of the measured sample, it will be a technique of choice when quantitative evaluation of mixtures is needed.
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Raman Characterization of Polymers in Industrial Applications
Raman Characterization of Polymers in Industrial Applications
Recent developments in Raman instrumentation have made the technique easier to use, more compact, and more affordable. Consequently, all of the demonstrated potential of the spectroscopy for industrial uses can now be exploited, including its use in combination with statistical methods for concentration calibrations.
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Real-time Monitoring of Polymerisations in Emulsions by Raman Spectroscopy - Modelling and Chemometrics
Real-time Monitoring of Polymerisations in Emulsions by Raman Spectroscopy - Modelling and Chemometrics
Raman spectra, in conjunction with Multivariate (Chemometric) Analysis, have been demonstrated to provide real-time information on the progress of a polymerisation reaction. As shown by this example, these results can provide unexpected information on the details of the reaction. in this case, the inequivalent reaction rates of the two monomers. Such information ultimately enables the process engineer to optimise his process.
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Using Chemometrics and Raman Spectra for Quantitative Predictions of Physical and Chemical Properties of Polymers
Using Chemometrics and Raman Spectra for Quantitative Predictions of Physical and Chemical Properties of Polymers
As far as polymeric fibres are concerned, slight modifications of Raman features are directly related to differences in the molecular orientation and the degree of crystallinity of the fibres. To utilize these subtle spectral changes and correlate them with physical properties of the polymer, one is obliged to use Chemometrics on the Raman spectra. The resulting synergism between Raman spectroscopy and Chemometrics will provide a powerful tool for monitoring and control of manufacturing of polymeric materials.
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Raman Imaging of Holographic Gratings Inscribed on Polymer Thin Films
Raman Imaging of Holographic Gratings Inscribed on Polymer Thin Films
Using holographic techniques we have structured the surface in a one step procedure (no wet nor photocuring processing ) along the X and Y directions. A grating is first inscribed with grooves along the X direction, the sample is rotated by 90° and a second grating is inscribed with grooves along the Y direction. The intensity of the 1st diffracted orders is monitored to have equal intensities in both X and Y directions.
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Valuable analysis tool for cosmetics and skin characterization
Valuable analysis tool for cosmetics and skin characterization
Confocal Raman Spectroscopy is an important analytical tool in various fields and it has shown high efficiency for specific applications in the cosmetic and/or pharmaceutical fields. Its non-invasive behavior, and its high molecular sensitivity make it one of the preferred label-free analytical techniques to characterize materials such as skin and cosmetic products.
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Milk compounds characterization by optical spectroscopies and laser diffraction
Milk compounds characterization by optical spectroscopies and laser diffraction
In the food industry, the compounds characterization is a critical step to ensure the quality of the products or to provide information to customers which can be sensitive to allergies. In this application note, we showed how optical spectroscopies and laser diffraction can help for food compounds characterization, especially on a specific product, i.e. milks.
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Pigment identification of an old Japanese flag “Hinomaru” using micro-XRF and Raman microscopy
Pigment identification of an old Japanese flag “Hinomaru” using micro-XRF and Raman microscopy
This application note introduces pigment identification using micro-XRF and Raman microscopy on a Japanese flag “Hinomaru”, which is presumed to be the oldest and designed by an ancient Japanese Emperor Go-Daigo. The two spectroscopic analyses identified that the red pigment used on the flag matched the cinnabar ore (Mercury(II) sulfide) mined from the Mine near the place where the Emperor lived.
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Morphological and chemical characterizations of microplastic particles using ParticleFinderTM and Raman techniques
Morphological and chemical characterizations of microplastic particles using ParticleFinderTM and Raman techniques
The assessment of microplastics in a marine environment is a multi-step process (sampling, extraction, detection and quantification of microplastics), in which each step is time consuming. Analyzing the chemical composition and morphology of microplastics represents a real challenge for answering crucial questions about the sources and fate of microplastics in aquatic environments. In this application note, we present a reproducible and time-effective method for fast and thorough morphological and chemical characterization of microplastics using a semi-automated scanning of particles coupled to micro-Raman spectroscopy. The rapid analysis of large number of collected particles allows for an exhaustive assessment of both large sample sizes and small subsamples.
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LabRAM HR Evolution Brochure
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1.25 MB
Technical Note: Spectral Resolution, Important Factors to Consider when Acquiring a Raman Spectrum
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2.81 MB
Technical Note: Improve your Raman System with Microscopy Options
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2.76 MB
Technical Note: The Importance of Confocality in Optical Microscopy and Raman Microscopy
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