This application reports on TERS characterization of binary phospholipid bilayer systems deposited onto a gold coated coverslip. Such samples are used as model samples for directly and chemo-selectively visualizing the distribution of a lipid constituent at the nanometer scale. In addition, nanomaterials have been introduced to mimic the interactions between nanoparticles and cellular membranes and better understand their toxicity on human health. The sensitivity of TERS for the detection and identification of NPs in phospholipid bilayers is demonstrated down to femtomolar concentration with a spatial resolution down to 7 nm. A specific liquid cell has also been developed to permit TERS measurements in solution and results of graphene oxide containing samples are presented.
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