Fluorescence

螢光計

Q: What is the difference between a fluorometer and a spectrofluorometer?

A fluorometer measures fluorescence intensity, while a spectrofluorometer measures fluorescence as a function of wavelength, providing complete excitation and emission spectra for more detailed analysis.

Q: Can one instrument perform both fluorescence and absorbance measurements?

Yes. Certain HORIBA systems, such as Duetta and Veloci , support both fluorescence and absorbance measurements within a single platform.

螢光計（和螢光計）測量不同螢光物體發出的螢光或光。當特定波長的光照射並激發樣品中的電子時，就會產生螢光，並且該樣品中的電子立即發射或發出不同波長的螢光。所有螢光物體都有自己的指紋，這意味著它會激發並發出可預測類型的光。

螢光樣品產生發射光譜，透過連接到類似於分光光度計的光電倍增檢測器的單色儀進行測量。

激發樣品後，螢光計使我們能夠獲得並測量樣品螢光的各種屬性，包括其強度、光譜波長和壽命。樣品的螢光可以間接探測樣品環境，描述溶劑-溶質相互作用、分子間和分子內相互作用等。螢光可用於樣本的定性和定量分析。

螢光光譜儀器提供的分析測量可用於多種應用，包括化學、生物化學、醫學研究、製藥、食品科學、環境研究和奈米技術。螢光光譜儀器已用於檢查假鈔、光電材料和蛋白質。

我們的螢光計可適應任何尺寸的工作區域，並可提供攜帶型或桌上型型號，或大型模組化系統，以滿足您的要求。此外，我們的螢光計可以連接成為模組化螢光計系統，或超連續光譜白光雷射驅動的螢光計！

HORIBA 的螢光計可提供低至皮秒的令人難以置信的快速壽命測定、深紫外吸光度以及從短至 200 nm 的紫外光一直到 1000 nm 的近紅外波長的螢光激發。

請參閱下文以了解我們的螢光計、組件和配件系列：

Frequently Asked Questions (FAQ)

A fluorometer measures fluorescence intensity, while a spectrofluorometer measures fluorescence as a function of wavelength, providing complete excitation and emission spectra for more detailed analysis.

Industries utilizing fluorescence spectroscopy include pharmaceutical development, biotechnology, environmental monitoring, academic research, semiconductor manufacturing, food science, nanotechnology, and materials science.

Fluorescence spectroscopy can detect naturally fluorescent compounds, fluorescent labels, proteins, nucleic acids, nanoparticles, dissolved organic matter, pharmaceuticals, and many other materials at very low concentrations.

Yes. Certain HORIBA systems, such as Duetta and Veloci, support both fluorescence and absorbance measurements within a single platform.