The HORIBA Scientific SPRi instruments are ideal solutions for label-free and multiplexed biomolecular analysis. These compact and flexible devices capable of either automated or manual operation are designed for easy determination of real-time interaction and kinetic studies. Their open formats enable numerous types of experiments covering biomolecular interactions, biochemistry, chemistry and physical-chemistry, to be explored without restriction or compromise.
Surface plasmon resonance imaging allows molecules binding study in label-free and real-time conditions. The power of the technique comes from its singularities, which are multiplexing and imaging, leading to a considerable speed-up of the processes’ analyses. This will be illustrated for antibody molecule study by three applications.
This article has been published in the Spring issue of the International Biopharmaceutical Industry (IBI) journal.
DARPins (Designed Ankyrin Repeat Proteins) are a class of non-immunoglobulin binders. Thanks to their specificity and robustness, they allow a multitude of novel, so far unfeasible applications. Surface Plasmon Resonance imaging (SPRi) is a powerful label-free technique that enables real-time target detection. Combining SPRi to massspectrometry (MS) allows biomolecules identification using their unique peptide mass fingerprint. In the past this combination was cumbersome and time-consuming. This application note shows how a protein kinase (RPS6KA2), a potential drug target is detected and identified using a hyphenated On-Chip SPR-MS coupling protocol, leading to saving time and money.