Label-free Detection / Surface Plasmon Resonance Imaging (SPRi)

The HORIBA Scientific SPRi instruments are ideal solutions for label-free and multiplexed biomolecular analysis. These compact and flexible devices capable of either automated or manual operation are designed for easy determination of real-time interaction and kinetic studies. Their open formats enable numerous types of experiments covering biomolecular interactions, biochemistry, chemistry and physical-chemistry, to be explored without restriction or compromise.

New publication: Antibodies exploration thanks to label-free SPRi

Surface plasmon resonance imaging allows molecules binding study in label-free and real-time conditions. The power of the technique comes from its singularities, which are multiplexing and imaging, leading to a considerable speed-up of the processes’ analyses. This will be illustrated for antibody molecule study by three applications.

This article has been published in the Spring issue of the International Biopharmaceutical Industry (IBI) journal.

 

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Streamlined SPRi-MS coupling to detect and identify a kinase in Cell lysate using the DARPins as binders

DARPins (Designed Ankyrin Repeat Proteins) are a class of non-immunoglobulin binders. Thanks to their specificity and robustness, they allow a multitude of novel, so far unfeasible applications. Surface Plasmon Resonance imaging (SPRi) is a powerful label-free technique that enables real-time target detection. Combining SPRi to massspectrometry (MS) allows biomolecules identification using their unique peptide mass fingerprint. In the past this combination was cumbersome and time-consuming. This application note shows how a protein kinase (RPS6KA2), a potential drug target is detected and identified using a hyphenated On-Chip SPR-MS coupling protocol, leading to saving time and money.

 

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How Making Screening is Easy Thanks to SPRimaging­: A Label-free Molecular Interaction Analysis Technology

Read a review on Surface Plasmon Resonance Imaging, a Label-Free Molecular Interaction technology.

Published on  www.biopharmaceuticalmedia.com

 

 

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