SPRi-Arrayer

SPRi Arrayer

Direct contact spotting

SPRi-Arrayer is an automatic, compact and portable apparatus. It prints DNA, oligonucleotides, proteins, bacterial clones and other materials with low cross contamination using Xtend metal-ceramic capillary pins. SPRi-Arrayer includes microarray design software and is integrated with microarray image processing and data analysis tools.

 

 

Segment: Scientific
Division: Molecular and Microanalysis
Base product

Benchtop SPRi-Arrayer for printing microarrays of biological samples on glass, gold surfaces, or on membrane substrates.

  • Easy to start
  • Fast and versatile
  • Prints onto SPRi-Biochip™, SPRi-Slides or any other standard slides
  • Prints up to 400 (20x20) samples on SPRi-Slides or SPRi-Biochip™
  • Spot size : from 140 to 500 µm, depending on matrix size

Arrayer SPRi

  • Print Method: Contact printing
  • Number of printing needles: 1-16 in various patterns
  • Slide capacity: 14 slides
  • SPRi-Biochip™ capacity: 8 biochips
  • SPRi-Slide capacity: 8 slides
  • Slide locks: Independent unit
  • Needle cleaning technology: Fluid stream washing, vacuum drying
Antibody-Antigen Specific interaction
Antibody-Antigen Specific interaction
Detection of birch pollen allergen in the air
Detection of birch pollen allergen in the air
Interaction between immobilized peptides and protein from sera on Cystamine / Glutaraldehyde functionalized biochip
Interaction between immobilized peptides and protein from sera on Cystamine / Glutaraldehyde functionalized biochip
Monitoring of interactions between aptamers and human IgE by Surface Plasmon Resonance imaging
Monitoring of interactions between aptamers and human IgE by Surface Plasmon Resonance imaging
Monitoring of interactions between aptamers and human IgE by SPRi
Monitoring of interactions between aptamers and human IgE by SPRi
Protein-Peptide interactions studies with SPRi
Protein-Peptide interactions studies with SPRi
Description of the advantages of combining the use of peptide chips with direct label-free detection as achieved by SPR imaging (SPRi).
SPRi Imaging of oligosaccharides proteins interactions
SPRi Imaging of oligosaccharides proteins interactions
Real time detection of lymphocytes with SPRi
Real time detection of lymphocytes with SPRi
Validation of the activity of G-protein-coupled receptors (GPCRs) using SPRi
Validation of the activity of G-protein-coupled receptors (GPCRs) using SPRi
Streamlined SPRi-MS coupling to detect and identify a kinase in Cell lysate using the DARPins as binders
Streamlined SPRi-MS coupling to detect and identify a kinase in Cell lysate using the DARPins as binders
DARPins (Designed Ankyrin Repeat Proteins) are a class of non-immunoglobulin binders. Thanks to their specificity and robustness, they allow a multitude of novel, so far unfeasible applications. Surface Plasmon Resonance imaging (SPRi) is a powerful label-free technique that enables real-time target detection. Combining SPRi to massspectrometry (MS) allows biomolecules identification using their unique peptide mass fingerprint. In the past this combination was cumbersome and time-consuming. This application note shows how a protein kinase (RPS6KA2), a potential drug target is detected and identified using a hyphenated On-Chip SPR-MS coupling protocol, leading to saving time and money.

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