DeltaTime

TCSPC Lifetime Kit

Fast, flexible and affordable plug-in TCSPC lifetime system for HORIBA Steady State Instruments, outperforming any other multifunctional fluorescence system on the market, integrating accessories with the widest array of picosecond light sources

The DeltaTime time correlated single photon counting (TCSPC) lifetime plug-in offers acquisition speed, flexibility, and affordability unavailable in any other multifunctioanl fluorescence solution on the market. DeltaTime seamlessly integrates monochromators, polarizers, and other accessories with the widest array of sources (LEDs, laser diodes, supercontinuum lasers) and detectors (including NIR), providing lifetime coverage from 25ps to 1sec over wavelengths spanning the UV to NIR.

The culmination of over 40 years of lifetime experience, DeltaTime highlights include: the fastest sources (up to 100 MHz), the widest lifetime ranges (ps to sec), virtually unlimited configurability and advanced lifetime analysis software. DeltaTime is part of our Delta series - truly the next generation of fluorescence lifetime systems.

Segment: Scientific

Division: Fluorescence Spectroscopy

Manufacturing Company: HORIBA Scientific

Fast…lifetime acquisition times from one millisecond
Sensitive…uses single-photon counting detection
Accurate…crystal locked timing circuits never require recalibration
Wide range…resolves lifetimes from 25ps to 1 second
Modular…easily reconfigured as measurement requirements evolve
Compact…desktop dimensions
Convenient…single USB 2.0 connection to PC

	DeltaPro-DD	DeltaPro-NL
Minimum lifetime	25 ps with laser-diode source	30 ps with laser-diode source
Shortest acquisition time	1 millisecond*	100 milliseconds*
Diode controller	DeltaDiode and SpectraLED	NanoLED and SpectraLED
Repetition rates	10 kHz–100 MHz with DeltaDiode* 0.1 Hz–10 kHz with SpectraLED	10 kHz–1 MHz with NanoLED 0.1 Hz–10 kHz with SpectraLED
Prompt FWHM	<200 ps FWHM with PPD and laser diode
Dead time	10 ns
Time ranges	10 ns – 11 s	100 ns–11 s
Wavelength selection	Interchangeable filters (filters purchased separately from HORIBA or others)
Detector response	250–650 nm standard; 250–850 nm and 300–900 nm optional
PC interface	USB 2.0. PC not included. Requires Windows^® XP or Windows^® 7, 32/64-bit English language ver.
System footprint	75 cm * 45 cm nominal excluding PC (depending on options)

Polarized light striking a fluorescent molecule results in polarized fluorescence. This polarized emission gradually returns to unpolarized fluorescence depending on rotational diffusion and other factors. Anisotropy is directly related to the polarization, and is the ratio of the polarized light component to the total light intensity.

Dye‐protein binding monitored in a microliter volume using timeresolved fluorescence

The potential health benefits stemming from the antioxidant activity of curcumin, commonly found in turmeric (Curcuma longa L), has attracted the interest of several research groups.

Stopped flow time‐resolved fluorescence study of serum albumin – curcuminoid binding

Rapid mixing accessories to perform stopped flow measurements have found application in characterizing interactions and reactions occurring in solution. Reactants are expelled from syringes, mixed and injected into a flowcell.

Measuring PL Upconversion Spectra and Lifetimes of Lanthanide-Doped Nanoparticles

Upconverting lanthanide-based nanomaterials exhibit a unique fluorescence anti-Stokes shift, which enables them to convert NIR wavelength excitation into visible shorter wavelength emissions (NIR to UV-Vis).

Characterizing Lanthanides in Glasses for Optical Applications

Glasses are essential materials with a multitude of uses and many forms. In the area of optoelectronics there is an interest to modify the glass composition to favor the incorporation of lanthanide elements.

Upconversion of Lanthanide-containing glasses using DD‐980L excitation

The phenomenon of upconversion is an optical process that takes in lower energy (longer wavelength) photons and emits higher energy (shorter wavelength) photons.

Measurement of carrier lifetime in perovskite for solar cell applications

Hybrid perovskite photovoltaics (PV) show promise because of their good efficiencies, which can be around 20%. Along with their PV characteristics, perovskite materials exhibit a high degree of radiative recombination.

Time‐resolved luminescence of security inks from the UV to NIR

The use of security features, such as luminescent inks, has increased significantly in an attempt to prevent fraud and counterfeiting of materials and goods.

Time‐resolved Fluorescence for Monitoring Food Composition

The use of time‐resolved fluorescence has expanded as the relative cost of instrumentation has decreased in recent years. One area where this is especially true is in the food industry, where time‐resolved fluorescence has been applied in the characterization of food stuffs as well as aspects related to food safety and degradation.

Monitoring Whole Leaf Fluorescence Using Time‐resolved Techniques

Light incident on a leaf can be absorbed by chlorophyll to commence the photosynthetic cycle. Excess energy can be liberated as heat or by emission of fluorescence and this can be used to assess the efficiency of the photosynthetic process.

The Measurement of Singlet Oxygen Lifetime Sensitized using Rose Bengal

The study of singlet oxygen (1O2) is of interest, principally, as it is a highly reactive species. It can be produced by photosensitisation, usually of a molecule such as a dye or porphyrin. Thus, by the appropriate selection of sensitiser, the presence of oxygen and light, 1O2 can be selectively generated. From a biological aspect it has the ability to damage and destroy cells, which has lead to interest in its use as an anticancer agent in photodynamic therapy (PDT).

Effect of temperature on HSA structure inferred using timeresolved room-temperature phosphorescence

To access intrinsic amino acids, such as tryptophan, as probes, the UV excitation wavelengths for pulsed phosphorescence measurements have long been the preserve of low-repetition-rate gas-filled lamps or larger laser systems. Recent developments have enabled the use of interchangeable semiconductor diodes...

Investigating photocleavage using time‐resolved emission spectra

The choice of protecting group is of crucial importance in the success of many steps in organic synthesis and the manipulation of polyfunctional molecules, since they can prevent the formation of undesired side products and reactions.

Elucidating Local Viscosity Using Fluorescence Lifetime Measurements

Certain fluorescent molecules, known as molecular rotors, can be employed to estimate the local (nanoscale) viscosity in microheterogeneous systems by measurement of their fluorescence lifetime. This can be advantageous over the usual fluorescence anisotropy method, as the measurement is simpler and faster to perform. This is demonstrated using the HORIBA Scientific TemPro fluorescence lifetime system to monitor the gelation of silica produced using the sol‐gel technique.

Tryptophan phosphorescence within protein molecules is gaining attention as a probe of protein dynamics and structure. The tryptophan phosphorescence lifetime, τ, varies with the protein molecule’s local environment and conformation.

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