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Universal Coupling Solution Microscopy Brochure
Universal Fiber Coupling Solution
Connect any of our steady state and hybrid fluorometers to virtually any upright or inverted microscope!
HORIBA Scientific’s confocal, universal fiber coupling solution allows any of our steady state and hybrid fluorometers to be easily connected to virtually any upright or inverted microscope. Spectrofluorometric capabilities include the unparalleled precision and sensitivity of Single-Photon Counting and the speed and sensitivity of CCD detection. Similarly, fluorescence spectral mapping capabilities utilize an automated fast scanning X-Y stage to generate rapid spectral maps with micron level spatial resolution. Of course our microscope solutions preserve the microscope's native optical performance and capabilities. For example, they add no optics to the entire emission optical path. Use the high performance cooled fluorescence camera to image your field of interest. Once you identify a region to spectrally analyze, simply switch your filter cube to a beam splitter or an appropriate dichroic, divert the light to the fluorometer, and you are collecting high resolution spectra. Custom solutions are also available to measure in the UV and NIR regions, beyond the normal range of your microscope.
Couple a microscope and:
- Measure complete spectra of samples as small as 1 μm.
- Get spectra from as little as a few molecules of sample.
- Perform localized FRET measurements.
Add an automated stage and camera, and:
- Create complete spatial/spectral maps.
- Perform repetitive QC characterization of structured samples like photovoltaics.
| Specification | Complete Microscope Solution | Interface to Existing Microscope |
|---|---|---|
| Objective | Plan achromat x10 and x60, other magnifications available. 6 position turret | User supplied |
| Confocal pinholes | Kinematic, 6 diameters, 0.1, 0.2, 0.4, 0.6, 0.8, 1 mm | Kinematic, 6 diameters, 0.1, 0.2, 0.4, 0.6, 0.8, 1 mm |
| Dimensions, Weight | 68 cm x 46 cm x 60 cm, 40 kg (BX53) 90 cm x 50 cm x 75 cm, 65 kg (IX73) | |
| Excitation source: Scopelite 200 (Optional) | ||
| Wavelength range | From 350 to 700 nm | From 350 to 700 nm |
| Illumination Adjustment | 5 preset % apertures: 0, 25, 50, 75, 100 | 5 preset % Apertures: 0, 25, 50, 75, 100 |
| Output Power | 7 Watts | 7 Watts |
| Lamp Life | 2000 hrs | 2000 hrs |
| Warm Up | 2 min | 2 min |
| Long Term Drift | <0.1% RMS | <0.1% RMS |
| Cooling | Air cooled by fan | Air cooled by fan |
| Dimensions | 20 x 14.5 x 25.4 cm (WxDxH) | 20 x 14.5 x 25.4 cm (WxDxH) |
| Weight | 3.4 kg | 3.4 kg |
| Filters | ||
| ND filter sliders | 6 positions: 0, 0.3, 0.6, 1, 2 and 3 OD | User supplied |
| Fibers | ||
| Multimode slit to round | UV-VIS : 1.5 m length, 250-850 nm wavelength range NIR : 1.5 m length, 350-2400 nm wavelength range | UV-VIS : 1.5 m length, 250-850 nm wavelength range NIR : 1.5 m length, 350-2400 nm wavelength range |
| Fiber Coupling | ||
| Excitation | Collimating lens adapters for Olympus | Collimating Lens Adapters for Olympus, Nikon, Leica and Zeiss |
| Emission | Universal C-mount adapter with pinhole holder | Universal C-mount adapter with pinhole holder |
| Camera | Required for mapping option | Required for mapping option |
| Fluorescence camera | 12 bits, 1.4 Mpix, cooled, low noise | 12 bits, 1.4 Mpix, cooled, low noise |
| Motorized stage | Required for mapping option | Required for mapping option |
| Repeatibility Travel range Manual control Automatic control | <1.0 µm 75 x 50 mm With joystick Through FluorEssence software | Suitable stage Through FluorEssence software |
| Software/Computer | ||
| FluorEssence PC requirements | Windows® 7, 32- or 64-bit At least three available USB ports 2 GB RAM 2 GB hard-disk space DVD-ROM drive Video resolution of at least 1280x800 | Windows® 7, 32- or 64-bit At least three available USB ports 2 GB RAM 2 GB hard-disk space DVD-ROM drive Video resolution of at least 1280x800 |
