Particle Analysis in Vaccine Manufacturing and Development
Size matters in vaccine delivery systems. Nanoparticles smaller than 200 nm generally present a greater immunogenic response than micro-particles larger than 1 micron.
ViewSizer 3000
Multi-Laser Nanoparticle Tracking Analysis (NTA)
Exosomes? Virus? Nanoparticle? Use multiple lasers for complete, detailed analysis of all the particles in your sample.
Exosomes, viruses, and nanoparticles all have wide size distributions which defeat traditional Nanoparticle Tracking Analysis (NTA) analyzers. The ViewSizer 3000 features simultaneous measurement with three lasers to collect the most accurate distribution and concentration information over a wide range of sizes within the same sample. Where the signal from a particle is too bright and saturates the detector from one laser, the software automatically uses data from a lower power laser to ensure the most accurate size and concentration information. On the other hand, when scattering from one laser is too weak for detection, the software uses data from a higher power laser to accurately track the particle.
Cross contamination is a concern in all analyses. Simplified cleaning means thorough cleaning. The easy-to-remove sample cells can be dissassembled for rapid, thorough cleaning, which leads to better data.
Escape the limits of traditional NTA
Accurate and sensitive analysis without cross contamination
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Segment: Scientific
Division: Particle Characterization
Manufacturing Company: HORIBA Instruments Incorporated
The ViewSizer 3000 uses the latest advances in nanoparticle tracking analysis (NTA) to accurately determine particle properties!
Size Measurement Range: 10 nm to 15 microns depending on sample
The ViewSizer determines particle size and size distribution details by multi-laser nanoparticle tracking analysis (NTA). Multiple lasers enable analysis of a broad range of sizes in the same sample.
Concentration Measurement Range: 5 x 106 to 2 x 108 particles/mL
NTA can be used to count particles in the measurement volume. A patented method corrects for the effect of particle size on effective measurement volume.
No cross-contamination
The sample cell can be completely removed and dissassembled for complete cleaning. Disassembly, cleaning, and reassembly is faster than rinsing a flow cell. Furthermore, multiple cells can be used for faster throughput or assigned to various groups in a shared (core) facility.
Featured Publications for the ViewSizer 3000:
| Biological characterization using protein crystal measurements | https://bioprocessintl.com/analytical/product-characterization/biological-characterization-using-protein-crystal-measurements/ |
| A lipase-independent pathway of lipid release and immune modulation by adipocytes | https://science.sciencemag.org/content/363/6430/989 |
| Application of a novel new multispectral nanoparticle tracking technique | https://iopscience.iop.org/article/10.1088/1361-6501/aab940/meta |
| Biophysical characterization of polydisperse liposomal adjuvant formulations | https://doi.org/10.1016/j.bbrc.2020.05.156 |
| Characterisation of particles in solution – a perspective on light scattering and comparative technologies | https://doi.org/10.1080/14686996.2018.1517587 |
| Cyclodextrin Reduces Intravenous Toxicity of a Model Compound | https://doi.org/10.1016/j.xphs.2019.01.004 |
| Development and anti-Candida evaluation of the vaginal delivery system of amphotericin B nanosuspension-loaded thermogel | https://doi.org/10.1080/1061186X.2018.1434660 |
| Electrochemical sensor based on F,N-doped carbon dots decorated laccase for detection of catechol | https://doi.org/10.1016/j.jelechem.2019.03.071 |
| Light scattering by pure water and seawater: the depolarization ratio and its variation with salinity | https://doi.org/10.1364/AO.58.000991 |
| Lipid Nanoparticle-Delivered Chemically Modified mRNA Restores Chloride Secretion in Cystic Fibrosis | https://doi.org/10.1016/j.ymthe.2018.05.014 |
| Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells | https://doi.org/10.1038/s41598-020-67039-w |
| Multifunctional Nanocomposites Based on Liposomes and Layered Double Hydroxides Conjugated with Glycylsarcosine for Efficient Topical Drug Delivery to the Posterior Segment of the Eye | https://doi.org/10.1021/acs.molpharmaceut.8b01136 |
| Particle size analysis of polydisperse liposome formulations with a novel multispectral advanced nanoparticle tracking technology | https://doi.org/10.1016/j.ijpharm.2019.06.013 |
| Review of nanoparticles in ultrapure water: definitions and current metrologies for detection and control | https://www.ovivowater.com/review-of-nanoparticles-in-ultrapure-water-definitions-and-current-metrologies-for-detection-and-control/ |
| Spark erosion as a high-throughput method for producing bimodal nanostructured 316L stainless steel powder | https://doi.org/10.1016/j.powtec.2018.01.012 |
| Synthesis and Characterization of EGFR-Targeted Immunoporphysomes | http://hdl.handle.net/1807/89548 |
| Synthesis of Ultrasmall Synthetic Melanin Nanoparticles by UV Irradiation in Acidic and Neutral Conditions | https://pubs.acs.org/doi/abs/10.1021/acsabm.9b00747 |
| Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles | Protocol (jove.com) | https://doi.org/10.3791/62447 |
| Isolation and characterization of EV in Saliva of Children with Asthma | https://evcna.com/article/view/3962 |
| Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential | https://doi.org/10.1016/j.bbi.2020.12.007 |
| Skeletal muscle tissue secretes more extracellular vesicles than white adipose tissue and myofibers are a major source ex vivo but not in vivo | https://doi.org/10.1101/2020.09.27.313932 |
| Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands | https://www.nature.com/articles/s41598-021-84809-2 |
| Purification of Cas9 - RNA Complexes by Ultrafiltration | https://doi.org/10.1002/btpr.3104 |
| Assessment of Surface Glycan Diversity on Extracellular Vesicles by Lectin Microarray and Glycoengineering Strategies for Drug Delivery Applications | https://doi.org/10.1002/smtd.202100785 |
| Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration | https://doi.org/10.1172/jci.insight.155928 |
| Circulating Extracellular Vesicles From Septic Mice Induce Brain Inflammation via Mirna Cargo | http://hdl.handle.net/10713/19055 |
Measurement Range | 10 nm to 15 μm |
|---|---|
Typical Sample Volume | 350 µL to 1.25 mL |
Typical Sample Concentration | 5 x 106 to 2x108 particles/mL |
Sample Temperature Range | 10° C to 50° C +/- 0.1° C |
Dimensions | 55 cm W x 66 cm D x 35 cm H |
Weight | 27 kg |
Operational Environment | 15° C to 30° C with < 85% RH |
Achieving Infectious Titer with Multi-laser Nanoparticle Tracking Analysis (NTA)
In this note, the upstream process of analyzing viral preparations is addressed, as well as the use of multi-laser nanoparticle tracking analysis (NTA) as a cost and time efficient method to measure size, count virus particles, and correlate to infectious titer.
Optimization of an Emulsion Polymerization Process and Product Through Nanoparticle Concentration Analysis
This note covers the history, theory and processes used for emulsion polymerization. It also examines the importance of measuring the concentration of latex nanoparticles produced by emulsion polymerization for a biotech application developed by the Gianneschi lab at UCSD.
Predicting Whiskey Shelf Stability with Particle Size Distribution
Careful control of the particulates in whiskey is an important step to quality flavor and color. Inadequate monitoring of particle size impedes the final product stability, consistency, quality, and price. In this note, the particle science behind these determining factors will be thoroughly examined and explained.
Optimization of High-Performance Nanostructured Powder Metallurgy Materials
In this note, we will briefly explore the history of powder metallurgy and then examine the importance of powder quality to the production of nanomaterials.
Nanobubble Size and Concentration Analysis with Multi-laser NTA
This note analyzes carbon dioxide and oxygen gas nanobubbles used for pain management to demonstrate multi-laser Nanoparticle Tracking Analysis’s ability to characterize a mixture of nano and microbubbles without sample manipulation.
Accurate Protein Aggregation Analysis
Protein aggregation is an important concern when developing and manufacturing biotherapeutics. It is important to quantify protein aggregation and particle formulation as this data is needed to guide formulation development, evaluate handling requirements, and monitor product quality.
Distribution and Concentration of Exosomes
In this study, we demonstrate the ViewSizer 3000’s capabilities as a next generation NTA based analysis instrument in order to accurately and efficiently measure and characterize exosomes via particle size and concentration.
Characterization of Sub-Visible Particles in Protein Therapeutic Formulations
The ViewSizer™ 3000 enables scientists to visualize and quantify the kinetics of protein aggregation in real-time for biologic therapeutics under a variety of stress conditions.
Nanoparticle Analysis
Nanotechnology is extremely diverse, ranging from novel extensions of conventional device physics, to completely new approaches based upon molecular self-assembly and to developing new materials with dimensions on the nanoscale. Materials reduced to the nanoscale can show different properties compared to what they exhibit on a macroscale, enabling unique applications.
