Aqualog

Water Treatment Plant Analyzer

A better, faster and economical method for monitoring organics

Aqualog® is the only instrument to simultaneously measure both absorbance spectra and fluorescence Excitation-Emission Matrices. EEMs are acquired up to a 100 times faster than with other instruments. Dedicated software automates traceable Quinine Sulfate Unit calibration and correction of inner-filter effects and Rayleigh and Raman scattering lines, enabling rapid export to modeling algorithms like PARAFAC.

Aqualog now offers new deep UV absorbance and fluorescence excitation from 200 nm, in addition to a fully automated sample queue tool to facilitate continuous data acquisition, analysis and export for immediate multivariate analysis with the Solo™ package from our partner, Eigenvector Research, Inc.

To see real world uses of the Aqualog visit our Science in Action Series page.

Learn how spectroscopy affects in everyday life with Spectroscopy Matters.

A-TEEM™ Molecular Fingerprinting Video (1:43)

Aqualog Data Stream and Sipper Video (1:57)

Spectroscopy in :60 - FAST -01 Autosampler (1:12)

EEM Acquisition Speed Comparison Video (1:08)

Life in the Arctic with the Aqualog (5:09)

Life in the Arctic with the Aqualog was created entirely by our customer who wanted to record her experience transitioning from a scanning fluorometer to the Aqualog for water research. She is solely responsible for its content.

Segment: Scientific

Division: Fluorescence Spectroscopy

Manufacturing Company: HORIBA Scientific

Hardware

The only true simultaneous absorbance-fluorescence system available
TE-cooled CCD fluorescence emission detector for rapid data acquisition up to 100 times faster than any other benchtop fluorometer
Corrected UV-VIS absorbance detection path for stability and accuracy
Double grating excitation monochromator for superior stray light rejection
Matching bandpass for absorbance and fluorescence spectra
Automatic sample changer option (2 or 4 position)
Compatible with flow cells and titrator

Software

Optimized experiment set-up menus minimize user configuration time
Complete NIST-traceable corrected fluorescence spectra automatically generated
Spectral and kinetic analysis tools for both absorbance and fluorescence data
Methods and batch protocols for automating multiple sample measurement

NEW A-TEEM™ technology

A-TEEM technology uses absorbance, transmittance and EEM data to fingerprint molecules with high specificity and ultrahigh-sensitivity at a 6 million nm/min emission scan rate!

All colored molecules exhibit unique molecular absorbance and transmittance spectra; many colored molecules also exhibit unique fluorescence excitation and emission spectra that can be measured as an excitation-emission matrix (EEM). Simultaneously combining Absorbance–Transmission and EEM is a new technique (A-TEEM) that provides a distinct molecular fingerprint with numerous potential applications.

Aqualog combines an ultrafast CCD that’s up to 4,000 times faster than traditional PMT-based fluorometers, with our new A-TEEM technology so you can easily and effectively identify, quantify and understand individual organic compounds in complex mixtures in minutes. A-TEEM has already proven in many cases to be more effective in protein, vaccine, wine and water research, quality and process applications than HPLC and vibrational spectroscopy.

NEW Aqualog® Datastream Dashboard

Features

Seamless integration with Aqualog
Convenient HTML-based interface
Push-button method operation
Simple administrator level controls for calibration and method development

Benefits

Easy access through internet or intranet
Dashboard shows the latest readings, time series and tables for trends and analysis
WTP can upload their own independent data

^{Aqualog Datastream Dashboard is powered by Solo_Predictor software from Eigenvector Research, Incorporated}

NEW Automatic Sipper Accessory

New for our Aqualog® A-TEEM™ spectrometer, the Aqualog automatic sipper accessory handles sampling from a single source, in addition to dispensing rinsing solutions, detergents and controlling reverse-flow drainage The 4-sample changer unitconnects to the main sipper unit to enable sampling of up to 4 sources.

The sipper offers convenient installation and operation, with built-in automatic cleaning, leak detection and protection. It is fully integrated into the new Aqualog 4.0 software for batch analysis and has a variety of uses in water, pharmaceutical, beverage phenolics and many other applications.

When used at a water treatment plant, the sipper and four channel accessory enable the Aqualog to automatically extract and monitor raw, settled and finished water samples. Each sample changer unit is compatible with overflow and filtration devices, serving up to 4 independent water treatment plant sources.

*Fluorescence Hardware*
Light source	Extended-UV: 150W vertically mounted xenon arc lamp
Excitation range	200 nm to upper limit of emission detector
Excitation bandpass	5 nm
Excitation monochromator	Subtractive double monochromator
Excitation gratings	1200 gr/mm, 250 nm blaze
Excitation wavelength accuracy	±1 nm
Choice of Detector	Red-extended
Emission range	250-800 nm
Emission grating	285 gr/mm; 350 nm blaze
Hardware pixel binning	0.58, 1.16, 2.32, 4.64 nm/pixel
Emission bandpass	5 nm
Emission spectrograph	Fixed, aberration-corrected 140 mm focal length
Emission detector	TE-cooled back-illuminated CCD
Emission integration time	5 ms minimum
CCD gain options	2.25 e-/cts in high gain, 4.5 e-/cts in medium gain, 9 e-/cts in low gain
Sensitivity	Water-Raman SNR > 20,000:1 (RMS method) (350 nm excitation, 30s integration)
Weight	32.72 kg (72 lbs)
Dimensions	LWH (618 x 435 x 336 mm); (24" x 17" x 13")

*Absorbance Hardware*
Scanning range	200-800 nm (UV lamp)
Bandpass	5 nm
Slew speed	Maximum 500 nm/s
Optical system	Corrected single-beam
Detector	Si photodiode
Wavelength accuracy	+/- 1 nm
Wavelength repeatability	+/- 0.5
Photometric accuracy	+/- 0.01 AU from 0 to 2 AU
Photometric stability	<0.002 AU per hour
Photometric repeatability	+/- 0.002 AU (0 to 1 AU)
Stray light	<1% measure with Kl standard

A-TEEM Fluorescence for Identification and Quantification of Fulvic Acid Adulteration in Commercial Humic Products

This app note demonstrates that the A-TEEM molecular fingerprinting technique can easily distinguish between different components in mixtures that contain FF and other ingredients considered to be adulterants, including lignosulphonates, organic acids, molasses, and provides a quantitative evaluation of mixtures.

Rapid Extra Virgin Olive Oil Classification and Blend Quantitation

The resurgence of interest in the Mediterranean Diet and its associated health benefits have directed focus on the role that Extra Virgin Olive Oil plays. The increasing awareness is leading to increasing product demand, but also opportunities to compromise quality. Hence the need for rapid analytical methods to perform Quality Analysis of various product samples.

Monitoring Cell Culture Media Variability using a Simple Optical Technique (A-TEEM Molecular Fingerprinting)

With the rise of protein production using mammalian cell culture, it has become increasingly important to control the quality of the cell culture media for use in production processes. Cell culture media are usually prepared as aqueous solutions and should provide everything a cell line needs for optimal cell growth as well as product yield and quality.

Classification and Phenolics Analysis of Red Wines with A-TEEM Molecular Fingerprinting

Aqualog®, an analytical instrument based on the simultaneous measurement of Absorbance, Transmission, Fluorescence Excitation and Emission Matrix (A-TEEM). A-TEEM fingerprints yield qualitative and quantitative composition of key flavor and color determinants in wine and spirits that are not discernible with simple Absorbance or Transmission data analysis.

Spectroscopic Analysis of Red Wines with Aqualog

HORIBA’s Aqualog is uniquely equipped with patented simultaneous Absorbance spectral and fluorescence excitation-emission matrix (EEM) technology which provides rapid access to a wide range of parameters important to commercial wine processing and quality characterization.

This study describes the application of simultaneous absorbance and fluorescence excitation-emission matrix (EEM) analysis for the purpose of identification and classification of freshwater planktonic algal species.

Holistic Analysis of Mammalian Cell Proliferation using Fluorescence Spectroscopy

EPA Stage 2 Disinfection Compliance with Aqualog

This application note describes the use of the Aqualog for monitoring regulated Dissolved Organic Matter (DOM) and disinfection by-product issues for drinking water treatment.

Fast & Non-invasive Determination of Skin State

Biological tissues contain chromophores that absorb light, as well as fluorophores that absorb and reemit light (fluorescence effect). Light absorption depends on the chromophores’ content and their distribution within the organic matter.

Insulin Structure and Stability Assessment

Stability and aggregation of insulin are studied using simultaneous fluorescence excitation emission matrices (EEMs) and UV-Vis absorbance spectroscopy [1]. Insulin is a protein-hormone, produced by the pancreas and is necessary for basic metabolic processes. The different types of commercial insulin therapeutics generally fall into two categories: short-acting and long-acting insulin. The difference between some short-acting and long-acting insulin is, in some cases, only one to three residues in the protein sequence.

Noninvasive In-Vivo Determination of Sunscreen-UVA Protection Factors

The development and evaluation of UVA (320–400 nm) sunscreens is important because UVA sunlight can penetrate deep into human skin and cause severe internal damage, as well as erythema and photoaging.

$Milk compounds characterization by optical spectroscopies and laser diffraction$

In the food industry, the compounds characterization is a critical step to ensure the quality of the products or to provide information to customers which can be sensitive to allergies. In this application note, we showed how optical spectroscopies and laser diffraction can help for food compounds characterization, especially on a specific product, i.e. milks.

Spectroscopic Methods for Sunscreens Characterization

This Application Note outlines three different kinds of spectroscopic tools being used for the characterization of sunscreens, and discusses the obtained results. These include Fluorescence spectroscopy for photoactivity, Particle Size analysis for composition and Raman microscopy for formulation investigation.

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Aqualog for Water Treatment Organics Monitoring Brochure