DeltaPro

HORIBA DeltaPro

TCSPC Lifetime Fluorometer

Compact, affordable and modular fluorescence lifetime spectrometer – time correlated single photon counting for any lab

The DeltaPro has taken the complexity of time correlated single photon counting (TCSPC) and made it simple and affordable. Any lab can exploit the power of fluorescence dynamics using TCSPC. This system enables the seamless measurement of luminescence lifetimes from picoseconds to 1 second, using our DeltaHub timing electronics.

The DeltaPro takes advantage of interchangeable pulsed laser-diode and LED light-sources. The NanoLED and DeltaDiode range of sources cover discrete emission wavelengths from 250 nm to the near-IR and enables measurement of lifetimes from ps to µs. By simply adding a SpectraLED source to the system, lifetimes of µs to 1 second can be measured. Lifetimes ranging over 11 orders of magnitude measured with one compact and affordable system.

The DeltaPro system is modular. It can easily be upgraded to incorporate motorized devices, such as automated focusing and motorized polarizers through the F-Link spectrometer bus.

Segment: Scientific
Manufacturing Company: HORIBA Scientific
  • Filter-based wavelength selection
  • Fast, reliable USB 2.0 interface ensures trouble-free data-transfer to laptop or desktop PC
  • Comprehensive analysis software
  • Detector range is 185 to 650 nm (Optional upgrade to 850 and 900nm)
  • Standard sample holder with magnetic stirrer and temperature sensor
  • Measure rotational correlation times using optional polarizers
  • Full range of DeltaDiode, NanoLED and SpectraLED diode light sources from UV to near-IR available as an option
  • Operation up to 100MHz with DeltaDiode light sources
  • F-Link spectrometer interface for plug-n-play upgrades
  • Measurement modes
    • Lifetime – measure 25ps to 1s
    • Kinetic TCSPC – 1 to 10 000 decays measured sequentially in 1ms to 1min per decay
    • Anisotropy – reconvolution analysis to resolve shorter rotational correlation times

 

Minimum Lifetime25ps with laser diode source*
Shortest measurement time1 millisecond*
Repetition rates10kHz - 1MHz with NanoLED, 10kHz - 100MHz with DeltaDiode, 0.1Hz - 10kHz with SpectraLED
Diode controllerDeltaPro-NL: NanoLED and SpectraLED. 
DeltaPro-DD: DeltaDiode and SpectraLED
Prompt FWHM<200ps FWHM with PPD/TBX and laser diode
Deadtime10ns
Time ranges10ns - 11s (DeltaPro-DD)
Wavelength selectionInterchangeable filters (not included), monochromators optional
Detector response250 - 650nm standard, 250 - 850nm and 300 - 900nm optional.
PC interfaceUSB 2.0.  PC not included.  Requires Windows XP or Windows 7, 32/64-bit English language ver.
System Footprint75cm x 45cm x 25cm nominal excluding PC

*dependent on sample and system configuration

Fluorescence Anisotropy Studies
Fluorescence Anisotropy Studies
Polarized light striking a fluorescent molecule results in polarized fluorescence. This polarized emission gradually returns to unpolarized fluorescence depending on rotational diffusion and other factors. Anisotropy is directly related to the polarization, and is the ratio of the polarized light component to the total light intensity.
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Dye‐protein binding monitored in a microliter volume using timeresolved fluorescence
Dye‐protein binding monitored in a microliter volume using timeresolved fluorescence
The potential health benefits stemming from the antioxidant activity of curcumin, commonly found in turmeric (Curcuma longa L), has attracted the interest of several research groups.
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Stopped flow time‐resolved fluorescence study of serum albumin – curcuminoid binding
Stopped flow time‐resolved fluorescence study of serum albumin – curcuminoid binding
Rapid mixing accessories to perform stopped flow measurements have found application in characterizing interactions and reactions occurring in solution. Reactants are expelled from syringes, mixed and injected into a flowcell.
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Measuring PL Upconversion Spectra and Lifetimes of Lanthanide-Doped Nanoparticles
Measuring PL Upconversion Spectra and Lifetimes of Lanthanide-Doped Nanoparticles
Upconverting lanthanide-based nanomaterials exhibit a unique fluorescence anti-Stokes shift, which enables them to convert NIR wavelength excitation into visible shorter wavelength emissions (NIR to UV-Vis).
Read more
Characterizing Lanthanides in Glasses for Optical Applications
Characterizing Lanthanides in Glasses for Optical Applications
Glasses are essential materials with a multitude of uses and many forms. In the area of optoelectronics there is an interest to modify the glass composition to favor the incorporation of lanthanide elements.
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Upconversion of Lanthanide-containing glasses using DD‐980L excitation
Upconversion of Lanthanide-containing glasses using DD‐980L excitation
The phenomenon of upconversion is an optical process that takes in lower energy (longer wavelength) photons and emits higher energy (shorter wavelength) photons.
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Measurement of carrier lifetime in perovskite for solar cell applications
Measurement of carrier lifetime in perovskite for solar cell applications
Hybrid perovskite photovoltaics (PV) show promise because of their good efficiencies, which can be around 20%. Along with their PV characteristics, perovskite materials exhibit a high degree of radiative recombination.
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Time‐resolved luminescence of security inks from the UV to NIR
Time‐resolved luminescence of security inks from the UV to NIR
The use of security features, such as luminescent inks, has increased significantly in an attempt to prevent fraud and counterfeiting of materials and goods.
Read more
Time‐resolved Fluorescence for Monitoring Food Composition
Time‐resolved Fluorescence for Monitoring Food Composition
The use of time‐resolved fluorescence has expanded as the relative cost of instrumentation has decreased in recent years. One area where this is especially true is in the food industry, where time‐resolved fluorescence has been applied in the characterization of food stuffs as well as aspects related to food safety and degradation.
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Monitoring Whole Leaf Fluorescence Using Time‐resolved Techniques
Monitoring Whole Leaf Fluorescence Using Time‐resolved Techniques
Light incident on a leaf can be absorbed by chlorophyll to commence the photosynthetic cycle. Excess energy can be liberated as heat or by emission of fluorescence and this can be used to assess the efficiency of the photosynthetic process.
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Visualizing dental caries using fluorescence lifetime microscopy
Visualizing dental caries using fluorescence lifetime microscopy
Teeth are naturally fluorescent and changes in their composition, caused by decay for example, affect their fluorescence behavior.
Read more
The Measurement of Singlet Oxygen Lifetime Sensitized using Rose Bengal
The Measurement of Singlet Oxygen Lifetime Sensitized using Rose Bengal
The study of singlet oxygen (1O2) is of interest, principally, as it is a highly reactive species. It can be produced by photosensitisation, usually of a molecule such as a dye or porphyrin. Thus, by the appropriate selection of sensitiser, the presence of oxygen and light, 1O2 can be selectively generated. From a biological aspect it has the ability to damage and destroy cells, which has lead to interest in its use as an anticancer agent in photodynamic therapy (PDT).
Read more
Visualizing local viscosity using fluorescence lifetime microscopy
Visualizing local viscosity using fluorescence lifetime microscopy
The use of fluorescent molecules, known as molecular rotors, is advantageous in estimating the local (nanoscale) viscosity in microheterogeneous systems, since it just requires the measurement of their fluorescence lifetime.
Read more
Effect of temperature on HSA structure inferred using timeresolved room-temperature phosphorescence
Effect of temperature on HSA structure inferred using timeresolved room-temperature phosphorescence
To access intrinsic amino acids, such as tryptophan, as probes, the UV excitation wavelengths for pulsed phosphorescence measurements have long been the preserve of low-repetition-rate gas-filled lamps or larger laser systems. Recent developments have enabled the use of interchangeable semiconductor diodes...
Read more
Plasmon enhancement of protein fluorescence by silver nanostructures
Plasmon enhancement of protein fluorescence by silver nanostructures
The use of metal surfaces in conjunction with fluorescence molecules employing a plasmon effect, sometimes referred to as metal enhanced fluorescence, can be advantageous because of the possible enhancement of photophysical properties. For example, the emission intensity of the fluorophore, can be improved.
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Investigating photocleavage using time‐resolved emission spectra
Investigating photocleavage using time‐resolved emission spectra
The choice of protecting group is of crucial importance in the success of many steps in organic synthesis and the manipulation of polyfunctional molecules, since they can prevent the formation of undesired side products and reactions.
Read more
Elucidating Local Viscosity Using Fluorescence Lifetime Measurements
Elucidating Local Viscosity Using Fluorescence Lifetime Measurements
Certain fluorescent molecules, known as molecular rotors, can be employed to estimate the local (nanoscale) viscosity in microheterogeneous systems by measurement of their fluorescence lifetime. This can be advantageous over the usual fluorescence anisotropy method, as the measurement is simpler and faster to perform. This is demonstrated using the HORIBA Scientific TemPro fluorescence lifetime system to monitor the gelation of silica produced using the sol‐gel technique.
Read more
MCS and Protein Phosphorescence
MCS and Protein Phosphorescence
Tryptophan phosphorescence within protein molecules is gaining attention as a probe of protein dynamics and structure. The tryptophan phosphorescence lifetime, τ, varies with the protein molecule’s local environment and conformation.
Read more
5.28 MB
Delta Series Brochure
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0.99 MB
DeltaPro Specification Sheet
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0.88 MB
Hybrid Picosecond Photon Detector (HPPD) TechNote PDF
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0.23 MB
Time‐resolved fluorescence lifetime measurements Technical Note PDF
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