Reagents for label-free interaction analysis

SPRi Blocking solutions

Our SPRi reagents have been especially designed for our SPRi systems. They allow you to work with confidence and save time for faster results.

Segment: Scientific
Division: Molecular and Microanalysis
Base product
Manufacturing Company: HORIBA France SAS

SPRi Student kits

The SPRi starting kits are used to get acquainted with multiplex SPR imaging. Choose from two biological models and learn how SPRi can save you time and money.

Product name

Description

Protein biochip kit

Ready-to-use SPRi-Biochips and necessary reagents to perform a antibody-protein interaction

DNA biochip kit

Ready-to-use SPRi-Biochips and necessary reagents to perform a DNA-DNA interaction

 

SPRi Running buffers

Depending on the ligand/analyte interaction study, the composition of the running buffer is expected to improve specific binding. Salted buffers like PBS or HEPES are recommended. Buffer composition, concentration or pH must be adjusted according to the biomolecules studied.

Product nameDescription
PBS0.01 M PBS pH 7.4, 137 mM NaCl,  2.7 mM KCl
PBS-T0.01 M PBS pH 7.4,  137 mM NaCl,  2.7 mM KCl, 0.005% (v/v) Tween 20
PBS 10X0.1 M PBS pH 7.4, 1.37 M NaCl, 0.027 M KCl 
PBS-T 10X0.1 M PBS pH 7.4 , 1.37 M NaCl , 0.027 M KCl,  0.05% (v/v) Tween 20
TBS 5X0.05 M TBS pH 7.4 , 150 mM NaCl
TBS-T 5X0.05 M TBS pH 7.4 , 150 mM NaCl, 0.005% (v/v) Tween 20
HBS0.01 M HEPES pH 7.4,  150 mM NaCl
HBS-T0.01 M HEPES pH 7.4 , 150 mM NaCl, 0.005% (v/v) Tween 20
HBS-E0.01 M HEPES pH 7.4,  150 mM NaCl, 3 mM EDTA
HBS-ET0.01 M HEPES pH 7.4 , 150 mM NaCl , 3 mM EDTA , 0.005% (v/v) Tween 20
SPRi buffer kit 1Contains 4 buffers without Tween
SPRi buffer kit 2Contains 4 buffers with Tween

 

SPRi Calibration solution

The calibration solution helps correct for sensitivity dispersions on the sensor chip surface.

Product nameDescription
N-SUC3 mg sucrose to reconstitute in 1 mL of running buffer

 

SPRi activation kits

The SPRi activation kits should be used for primary amine coupling chesmitries with COOH groups ready to be activated (SAM CH-HD and CH-LD and 3D chemistries)

 

Product nameDescription
Amine coupling kit 1Necessary reagents for NHS activation of COOH groups for amine coupling. To be reconstituted in 1 mL of filtered and deionised water.
Chemistry activation using NHS is more stable but less reactive.
Amine coupling kit 2Necessary reagents for Sulfo-NHS activation of COOH groups for amine coupling. To be reconstituted in 1 mL of filtered and deionised water.
Chemistry activation using Sulfo-NHS is more reactive but less stable

 

SPRi Blocking solutions

The SPRi blocking solutions are used for deactivating the remaining reactive groups of the surface chemistry for covalent coupling. They can also help reduce non-specific binding.

Product nameDescription
B-Glycine10 mM Glycine in PBS
B-Ethanolamine1M Ethanolamine-HCl pH 9
B-BSA1% BSA in PBS-T

SPRi Regeneration solutions

The SPRi regeneration solutions are used to remove the bound analyte and any other non-covalently bound material while keeping the ligand activated. Regeneration conditions (concentration, contact time, pH…) must be optimized for each new ligand-analyte interaction study.

Product nameDescription
Glycine-HCl100 mM Glycine - HCl pH 2
SDS10% SDS
NaOH50 mM NaOH
HCl100 mM HCl
NaCl1 M NaCl
SPRi Regeneration scouting kitContains 5 regeneration solutions,
for exploring regeneration conditions.

 

SPRi Cleaning solutions

The SPRi cleaning solutions are used for the daily cleaning and monthly maintenance of the SPRi systems.

Product nameDescription
C-NaOH100 mM NaOH (for the monthly maintenance)
C-HCl100 mM HCl (for the monthly maintenance)
SDS10% SDS (for the daily cleaning)
SPRi Cleaning kitContains 4 cleaning solutions

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